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M Montenarh

Publications and source records attributed to M Montenarh.

76 records · Page 5Linked to original sources
PubMedDate not supplied

Serum anti-p53 antibodies in patients with lung cancer.

Antibodies against the p53 protein are produced by some cancer patients. In some tumour entities, the presence of p53 autoantibodies have been linked to poorer survival. This study was designed to assess the prevalence and prognostic implications of p53 autoantibodies in patients with lung cancer. Serum samples of 180 patients were tested for antibodies against p53 protein using an ELISA. We studied 134 patients with primary lung cancer [histology: small cell lung carcinoma (SCLC) n=35; non-small cell lung carcinoma (NSCLC) n=99]. The control group consisted of 46 patients without lung cancer. In 17/134 (12.6%) of the cancer patients, p53 autoantibodies were detected (4/35 SCLC, 13/99 NSCLC). Most of the positive results were found in advanced stages of NSCLC (stage I-IIIA: 1/34; stage IIIB/IV: 12/65). One of the 46 control patients tested positive. Statistical analysis of survival shows no correlation with p53 antibody status in SCLC, but a significant correlation with shorter survival in NSCLC (p=0.01). After correction for stage of disease this correlation remains significant (stage IIIB/IV: p=0.02). In our series, the presence of anti-p53 autoantibodies is almost exclusively linked to the presence of malignant disease. Prognosis for patients with NSCLC, but not SCLC seems to be linked to the p53 autoantibody status.

Autoantibodies↗
PubMedDate not supplied

Analysis of the immunoreactivity of three anti-p53 antibodies and estimation of the relations between p53 status and MDM2 protein expression in ovarian carcinomas.

BACKGROUND: The p53 growth suppressor is inactivated in human tumors by several distinct mechanisms, such as point mutations, binding to viral proteins and association with the MDM2 protein. Little is known about the expression of different immunologically distinct forms of p53 and MDM2 protein in human tumors and especially in ovarian carcinomas. MATERIALS AND METHODS: The overexpression of p53 and MDM2 oncoproteins was examined in a series of 46 ovarian carcinomas, taking into account the conventional pathological variables. The comparison of p53 and MDM2 expression in tissue sections and respective cyst and-or ascitic fluid cells was also performed. For the determination of the p53 expression the reactivity of three commonly used anti-p53 antibodies (DO7, PAb240, PAb1620), which detect immunologically distinct subclasses of p53, were analyzed in relation to the MDM2 status in individual patients. RESULTS: The detection of the p53 expression was clearly related to the antibody applied. DO7 antibody appears to be superior to both PAb240 and PAb1620 in immunohistochemical tests. Nuclear MDM2 protein overexpression was found in 17.4% of cases and usually it was associated with p53 accumulation. There was no significant correlation between p53 as well as MDM2 expression and histological subtypes, staging and grading parameters of carcinomas however p53 accumulation was detected more often in III/IV than in I/II FIGO stages. CONCLUSION: In ovarian carcinomas significant inter- and intratumoral heterogeneity in p53 and MDM2 expression was identified and different MDM2/p53 phenotypes were revealed. The restriction of MDM2 overexpression to a small subset of neoplasms indicates that this oncoprotein plays a minor role in ovarian carcinogenesis.

Adenocarcinoma, Mucinous↗