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Biomedical subjects

M Montard

Publications and source records attributed to M Montard.

At least 55 records · Page 3Linked to original sources
PubMed1987

[The Octopus G1 program. Description and use].

The Octopus global analysis Program G1 was developed as a glaucoma program. It is the combination in a single program of: examination procedures, visual field assessment methods. The whole examination is carried out in three phases: the first phase measures 59 test locations in the central 30 degrees field. All locations are measured with the normal strategy. The local threshold is precisely determined to +/- 1 dB by the proven bracketing method. At the end of the first phase, the system automatically calculates the key values (visual field indices) for mean defect and loss variance. the second phase, which retests the same central field, starts from the threshold values measured during phase one. The additional indices corrected loss variance and short term fluctuation, are calculated. in the last phase 14 selected peripheral points between 30 and 60 degrees excentricity with particular emphasis on the superior and inferior nasal step are determined in a qualitative test. Visual field changes can be detected and categorized in three major groups: (Flammer and coll., 1985). 1. local defects or scotomas in varying depths and sizes. 2. diffused depression of the whole field. 3. Increased short-term. The original index called Corrected Loss Variance could determine an early defect before the existence of a light scotoma. Particularly, an increased Corrected Loss Variance indicates that the deviations are due to real scotomas and not just to scattering effects. The therapeutic decision could be more rational.

Diagnosis, Computer-Assisted↗
PubMed1987

[Preservation of human corneas in an enriched culture medium at +37 degrees Centigrade: histological and biochemical analyses].

Using the methods of Doughman, Sperling and Pels 66 human corneas were preserved up to five weeks in a modified tissue culture medium at +37 degrees C. The organ culture medium consisted of the following ingredients: Dulbecco Iscov's medium, 2% fetal calf serum, penicillin 100 IU/ml, Streptomycin 0.5 mg/ml, and fungizone 5 mg/ml. After culture the corneal endothelium was examined by light microscopy after staining with trypan blue 0.3% and alizarin red 1%. The number of dead cells was counted and the morphological alterations were described at 7, 14, 21 and 35 days. Biochemical analysis of the medium (pH, lactate, glucose) during storage has allowed the comparison of three kinds of storage: 50 ml, 20 ml, and 20 ml with 10 ml substitution weekly. After culture the number of dead cells did not exceed 1% at each period indicating that no dead cell was present at that time. Alteration of the cell shape and formation of rosette and joint meetings of 5 to 8 cells were the dominating morphological changes of the endothelial cells. The endothelial layer was intact and coherent on to 35 days culture. Endothelial cells loses during culture were not determined. The biochemical studies have shown the better quality (pH stability, anaerobic ratio) of storage in 50 ml medium or 20 ml 50% weekly substitution medium. This last kind of storage has given the best metabolic conditions for the preserved corneas. However for long period of storage up to 3-4 weeks, renewing 50% of the medium is found appropriate. At the end of this study a clinical trial is proposed.

Cornea↗
PubMed1986

[Intracameral penetration of pefloxacine in man].

Authors have studied the intraocular penetration of a new antibiotic, pefloxacin in 35 patients (35 eyes). This study emphasizes the three main qualities of this recent quinolone in ophthalmology: wide spectrum adequate for the most pathogens commonly found in endophthalmitis. Very large penetration in the aqueous humour flow with an average level of 0.95 mu/ml, which is over the minimal inhibitory concentration for many bacterial infections and is equal to 25% of average serum level.

Aged↗
PubMed1986

[In vitro semi-automated morphometry of preserved human corneal endothelium].

Cellular morphometry allows us to quantify the structural alterations of the corneal endothelium. Our study was a semi-automatized in vitro analysis of fresh and preserved corneal endothelium (MK medium cryopreservation). The equipment used was the Quantimeter 720 connected to a Digital PDP1123 computer. Nineteen corneas were studied and the following cellular parameters were established: perimeter, surface area, form factor and polymorphism coefficient. The computer program used was the SPSS/PC (Statistical Package for the Social Sciences) and the principal statistical tests were the Bartlett test and variance analysis. The statistical computerized study allowed us to compare the four cellular parameters and to establish the incidence rate of the conservation process on endothelial morphometry. The statistical and morphometry methods have proved to be reproducible and reliable and carry a supplementary quantitative element to analyse the influence of the conservation process.

Adult↗