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Biomedical subjects

M Monden

Publications and source records attributed to M Monden.

At least 433 records · Page 24Linked to original sources
PubMed1988

Extracellular calcium protects cultured rat hepatocytes from injury caused by hypothermic preservation.

Effects of various preservation solutions were compared in an experimental hypothermic preservation model using cultured rat hepatocytes. Hepatocytes prepared by the collagenase perfusion method were cultured for 48 hr, then the medium in each culture dish was exchanged for various preservation solutions, and the dishes were hypothermically (0-2 degrees C) stored in a refrigerator for 12-72 hr. After the preservation period, the hepatocytes were cultured again at 37 degrees C for 2 hr. Hepatocytes' viability after 18-hr preservation and reculture was greater when they were preserved in "intracellular" rather than "extracellular" solutions. Even with Euro-Collins solution (intracellular solution), hepatocyte viability decreased to approximately 20% after 24-hr preservation, and an increase in the cellular lipid peroxide content was observed. However, when this solution contained a submillimolar concentration of calcium, lipid peroxidation was significantly suppressed and hepatocyte viability was dramatically improved. Vitamin E was almost equally effective and a marked synergistic effect was observed with calcium. Calcium was found to be capable of maintaining the cellular glutathione level during cold storage, which seems to suppress lipid peroxidation and consequently improve hepatocyte survival.

Animals↗
PubMed1988

Necrosis of portal tumor embolus of hepatocellular carcinoma by lipiodol transcatheter chemo-embolization. A case report.

We describe a case of hepatocellular carcinoma in which a tumor embolus in the portal vein and 3 of 4 intrahepatic metastases were necrosed completely by Lipiodol transcatheter chemo-embolization (Lipiodol-TCE). Tumor emboli in the portal vein and intrahepatic metastases usually cannot be necrosed by conventional transcatheter chemo-embolization alone, because small nodules such as intrahepatic metastases and tumor emboli in the portal vein are supplied blood from the portal vein. However, in this case, Lipiodol-TCE was effective against tumor emboli in the portal vein and intrahepatic metastases.

Aged↗
PubMed1988

[Transarterial segmental hepatic chemo-embolization in patients with hepatocellular carcinoma].

We can administer a Lipiodol-adriamycin emulsion transarterially into the portal vein, because more than a certain amount of Lipiodol injected into the hepatic artery enters the portal vein through arterioportal communications without arterioportal shunting. When Gelfoam embolization is followed by portal venous administration of this emulsion, hepatic chemoembolization is performed from both artery and portal vein simultaneously. But this chemoembolization must be in a limited area of the liver, since such treatment of the whole liver is lethal. We encountered a segmental hepatic chemoembolization in four patients with hepatocellular carcinoma by the catheterization of a hepatic segmental artery. In two cases operated after segmental chemoembolization, complete necrosis was certified in the main tumor and daughter nodules. Hepatic segmental atrophy and infarctions were also noted in the treated segment. In two non-operated cases, hepatic segmental atrophy and excellent antitumor effects were demonstrated by CT and radioisotope liver scan. Thus, we call this treatment "transarterial partial hepatectomy".

Aged↗
PubMed1987

Effector cells in allelic H-2 class I-incompatible skin graft rejection.

The cellular mechanisms of skin graft rejection with allelic H-2 class I differences were studied by examining the effect on graft survival of in vivo administration of anti-Lyt-2.2 mAb, anti-L3T4 mAb, or both to recipient mice. The injections of anti-Lyt-2.2 mAb and anti-L3T4 mAb caused selective depletions of Lyt-2+ cells and L3T4+ cells, respectively. Injection of anti-Lyt-2.2 mAb significantly prolonged graft survival in 7 of 12 combinations of H-2D-end difference, but did not prolong graft survival in 5 other combinations of H-2D-end difference, or in 2 combinations of H-2K-end difference. Injection of anti-L3T4 mAb did not prolong graft survival in any combinations with class I difference tested. Injection of anti-L3T4 mAb plus anti-Lyt-2.2 mAb markedly prolonged graft survival in the combinations with class I difference in which anti-Lyt-2.2 mAb had no effect and overcame the effect of anti-Lyt-2.2 mAb in those in which anti-Lyt-2.2 mAb had an effect in prolonging graft survival. These results indicated that in combinations in which anti-Lyt-2.2 mAb did not prolong graft survival, class I antigen stimulated L3T4+ effector cells when Lyt-2+ cells were blocked and Lyt-2+ effector cells when L3T4+ cells were blocked. On the other hand, in the combinations in which anti-Lyt-2.2 mAb prolong graft survival, these antigens initially caused preferential stimulation of Lyt-2+ but not L3T4+ effector cells, although delayed activation of L3T4+ effector cells occurred when Lyt-2+ cells were blocked. Furthermore, a significant correlation was found between the effect of anti-Lyt-2.2 mAb in prolonging graft survival and the failure of recipient mice to produce H-2 antibody. These results can be taken as evidence that L3T4+ effector cells are not involved in the initial phase of graft rejection in these combinations.

Alleles↗
PubMed1987

Prolonged survival of hamster-to-rat liver xenografts using splenectomy and cyclosporine administration.

The immunosuppressive effect of splenectomy, alone or in combination with cyclosporine (CsA), was examined in hamster-to-rat orthotopic liver xenografts. The mean survival time was 7.3 +/- 0.5 days in untreated controls, 7.6 +/- 0.8 days with 40 mg/kg/day CsA, 7.2 +/- 0.4 days with splenectomy alone, and 17.6 +/- 5.6 days with splenectomy combined with 30 mg/kg/day CsA (P less than 0.01). The longest survival time was 27 days in this group. Marked enlargement of the spleen and high lymphocytotoxic antibody titer were characteristic of the unmodified recipients and those treated with CsA alone. Splenectomy by itself decreased the antibody formation without improvement of graft survival. In animals treated with the combined regimen, the lymphocytotoxic antibody titer was significantly suppressed, and the PMN and round cell infiltration were greatly reduced. Therefore, a synergistic effect was postulated between cyclosporine and splenectomy in this liver xenograft system.

Animals↗
PubMed1987

Correlation of regional insulin and glucagon content in canine pancreas and a possible interaction of A and B cells.

Canine pancreases divided into 8 anatomical subportions were examined for their regional insulin and glucagon content. Insulin content in the pancreas was gradually increased in the left lobe to about twice as much as that in the uncinatus, while glucagon content was increased steeply in the left lobe to 16 times as much as that of the uncinatus. Although hormonal content differs according to the anatomical area, a statistically significant positive correlation of mean regional insulin and glucagon content was observed (r = 0.994, p less than 0.001). A constant ratio of these two hormones suggests the functional coupling of A and B cells within the islets or in a given pancreatic subportion.

Animals↗
PubMed1986

[Effect of propranolol on intractable ascites following liver resection].

Hepatic and respiratory failure, common complications following liver resection for hepatocellular carcinoma (HCC), especially when it is combined with liver cirrhosis, can be overcome by careful management of the circulatory and respiratory systems. Another common complication is intractable ascites which resists conventional therapy, such as, diuretics and protein replacement. Here we report a case in which intractable ascites was successfully treated with propranolol. The patient, a 48-year-old man who underwent liver resection for HCC combined with cirrhosis, started to suffer from ascites about 1 week after surgery. Upon administration of propranolol (1 mg/kg/day) with furosemide, his body weight decreased 500 g/day, returning to the preoperative value in 2 weeks in parallel with the normalization of the PRA. No side effects were observed during the medication period. Propranolol, a beta-adrenergic antagonist, is thought to suppress renin secretion from the juxtaglomerular apparatus in the kidney by blocking its beta-adrenergic receptor, thus suppressing the entire renin-angiotensin-aldosterone system. We concluded that propranolol is a promising drug for intractable ascites encountered with liver cirrhosis.

Adrenergic beta-Antagonists↗
PubMed1986

[Pathogenesis and selection of treatment modalities for hepatocellular carcinoma].

Factors determining the prognosis of patients with hepatocellular carcinoma (HCC) were analyzed. Small HCC was compared to large HCC. Small HCC is less encapsulated, but has less frequent vascular invasion with favorable prognosis if resectable. Liver transplantation would be indicated in small HCC, unresectable because of far-advanced liver cirrhosis.

Carcinoma, Hepatocellular↗
PubMed1986

[A case of multiple liver metastases from sigmoid carcinoma treated successfully with arterial infusion chemotherapy].

A 63-year-old female, who had undergone sigmoidectomy for sigmoid carcinoma one year before, was admitted for multiple liver metastases. A urokinase-immobilized catheter was introduced into the proper hepatic artery via the gastroepiploic artery operatively. A daily arterial infusion of 5-FU (250 mg) was combined with a weekly arterial infusion of adriamycin (30 mg) or MMC (10 mg). After discharge, 5-FU (200 mg/day) was given orally and MMC (10 mg) was infused intraarterially every other week at an outpatient clinic. ADR or MMC was infused with angiotensin II, known to increase arterial blood supply to a malignant lesion. Ultrasonography demonstrated 35 to 50% reduction in tumor diameter. The density of metastases seen in computerized tomography became low indicating tumor necrosis. Plasma CEA level, which had initially been as high as 864 ng/ml, decreased rapidly and has remained within normal limits up to the present time. Arterial infusion chemotherapy using 5-FU, ADR or MMC in combination with angiotensin II seems to be effective in the treatment of multiple hepatic metastases from colorectal carcinoma.

Adenocarcinoma↗
PubMed1985

[Preoperative chemo-embolization for treatment of hepatocellular carcinoma].

A multidisciplinary treatment for hepatocellular carcinoma including preoperative chemo-embolization with resection treatment was introduced. Recent attempts at combining lipiodol with chemo-embolization have been quite promising. An ideal multidisciplinary treatment should include an effective device using the transportal approach.

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