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Biomedical subjects

M Mizugaki

Publications and source records attributed to M Mizugaki.

At least 181 records · Page 10Linked to original sources
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Extra-weak chemiluminescence of drugs. VIII. Extra-weak chemiluminescence arising from the amino-carbonyl reaction.

Extra-weak chemiluminescence (CL) from amino-carbonyl reactions of L-lysine with various sugars and aldehydes in aqueous solution was examined. Amongst the aldehydes and sugars tested, glycoaldehyde and D-arabinose produced the highest CL intensity. The CL of the amino-carbonyl reaction reached a maximum after about 60 minutes. The CL was pH dependent, and a linear relation between CL intensity and hydrogen-ion concentration was demonstrated. Low oxygen levels inhibited CL and no CL was produced in nitrogen purged solutions. Addition of cupric or ferrous ion, decreased the CL. The involvement of free radical intermediates was demonstrated by ESR. Our findings suggest that the CL of the amino-carbonyl reaction arises from free radicals derived from melanoidines or their intermediates. CL should prove useful for evaluating the stability of crude drugs extracted from natural resources that contain various amino acid derivatives protein and sugar components.

Aldehydes↗
PubMedDate not supplied

Extra-weak chemiluminescence of drugs. XI. Quenching effects of purine and pyrimidine derivatives on the extra-weak chemiluminescence derived from the Maillard reaction.

Quenching effects of purine and pyrimidine derivatives on the extra-weak chemiluminescence (CL) derived from the Maillard reaction of L-lysine with D-arabinose were investigated. The pyrimidine derivatives 2'-deoxy cytidine, uridine, and uracil quenched the CL. Cytidine did not quench the CL. Purine derivatives, e.g. uric acid and 1-methyl adenosine were particularly effective in quenching the CL. 5-Methyl adenine and xanthine also quenched the CL, but adenosine had no effect. A comparison of the CL-quenching abilities of compounds that have common basic structure was made; those with ribose at the 5-position were the strongest quenchers. A linear relationship between CL-quenching activity and the HOMO energy of the pi orbital for the various compounds was shown.

Arabinose↗