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Biomedical subjects

M Miyairi

Publications and source records attributed to M Miyairi.

31 records · Page 2Linked to original sources

Study on the fixation method of microcirculation system for electron microscopy.

1) Although glutaraldehyde solution was injected into the superior mesenteric artery, vessels and blood cells were not fixed immediately. 2) Glutaraldehyde was considered not to be suitable for the fixation of vessels and blood cells. 3) To observe the physiological and topographical correlation of vessels and blood contents, drop osmium fixation method was better to fix the microcirculatory units immediately. 4) The perfusion of glutaraldehyde was supposed to be necessary for the fixation of the solid tissue in which microcirculatory systems exist in deeper places like liver. However, the findings observed by the perfusion method might reflex microcirculatory disturbances occured by the perfusion of glutaraldehyde itself.

Animals↗

Budd-Chiari syndrome associated with protein-losing enteropathy.

Budd-Chiari syndrome associated with protein-losing enteropathy is reported. A 26-year-old male suffered from ascites, edema in the lower extremities, and engorgement of ascending veins on the abdominal wall. The diagnosis of Budd-Chiari syndrome was made by inferior vena cavography. The 131I-polyvinylpyrrolidone test showed the association of protein-losing enteropathy. The surgical operation was performed successfully, resulting in a marked improvement of signs and symptoms. To date, no similar case has yet been reported. There are, however, a number of reported cases of hypoproteinemia in Budd-Chiari syndrome, which might have been associated with a protein-losing enteropathy.

Adult↗

A study of bile canalicular contractions in isolated hepatocytes.

Living hepatocytes observed under standard methods of cell isolation and culture show frequent active contractions of bile canaliculi when viewed by time-lapse cinephotomicrography. This report gives details of the methods used to show the contractions. In addition, the characteristics of the contraction pattern are defined and analyzed. The contractions are regular, canalicular contraction time (systole) lasts 60 sec, and the most probably interval between contractions is 5 1/2 min. The finding of a regular pattern of contraction suggests an ordered, programmed, and integrated mechanism which entails a high degree of communication and functional coordination between neighboring hepatocytes.

Animals↗