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Biomedical subjects

M Mitsuhashi

Publications and source records attributed to M Mitsuhashi.

105 records · Page 6Linked to original sources
PubMed1985

Nonimmunologic activity of Dermatophagoides farinae extract.

Nine healthy and seven mite-sensitive allergic subjects were examined by skin sensitivity and IgE antibody to fractions from Dermatophagoides farinae, including high and low allergenic fraction to human. In the intracutaneous test, all healthy subjects reacted to 200 ng and in particular, five of them to 0.2 ng of Fr void on Sephadex G-100 filtration which was a low allergenic fraction. Furthermore, end points of intracutaneous test and specific IgE antibody titers did not correlate on Fr void. Conclusively, Dermatophagoides farinae extracts contain a nonimmunologically highly skin-reactive fraction.

Adult↗
PubMed1983

A unique substance from the granary weevil: nonspecific immunoglobulin binding substance.

We have studied the precipitation test as a diagnostic procedure for hypersensitivity pneumonitis or associated allergic states using Sitophilus granarius (SG) antigen. The incidence of positive reaction in the precipitation test against SG is extremely high in children and adults, not only asthmatics, but also in normal, healthy subjects. In experiments with radioimmunoassay using SG paper discs and 125I-labelled IgG fragments, SG was more strongly bound to the Fab than to the Fc fragment. However, the binding was not mediated by a true antigen-antibody reaction because the reaction was inhibited by both kappa- and lambda-type Bence-Jones proteins. Nonspecific immunoglobulin binding substance (IBS) was separated from crude SG extract by affinity chromatography using IgG immunosorbent. IBS was monocomponent on polyacrylamide gel electrophoresis in sodium dodecyl sulfate, and the molecular weight was about 12,000-14,000. We believe that IBS might be a valuable tool for immunological experiments, comparable with staphylococcal protein A.

Alveolitis, Extrinsic Allergic↗
PubMedDate not supplied

The elevated mRNA content as a potential indicator of precancerous states of the gallbladder mucosa in patients with pancreaticobiliary maljunction.

Pancreaticobiliary maljunction (PBM) patients have a high incidence of cancer in the gallbladder. In the present study, we showed that precancerous lesions in the gallbladder of PBM such as hyperplasia or metaplasia, indicated high concentration of the amount of total mRNA. The mRNA content could be easily measured by our new technique utilizing a GenePlate, which is a poly (dT) oligonucleotide immobilized plastic plate, and Yoyo-1, a fluorescent DNA intercalator. To standardize the mRNA contents, we utilized which was defined as the ratio of total mRNA contents to total nucleic acids in a same sample. The mRNA index of the proliferating cells was significantly higher than that of the resting cells, and the mRNA index of cancer cells, such as gastric cancer, colon cancer, bile duct cancer, and gallbladder cancer also showed significantly higher concentration than that of normal mucosa. In the study of precancerous lesion, PBM, the mRNA index was significantly higher than that of mild cholecystitis but less than that of gallbladder cancer, suggesting that in the gallbladder mucosa of PBM, a precancerous state was able to be diagnosed by the mRNA index.

Animals↗