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Biomedical subjects

M Miranda

Publications and source records attributed to M Miranda.

At least 181 records · Page 10Linked to original sources
PubMed1978

Nutritional inhibition of genetically determined renal disease and autoimmunity with prolongation of life in kdkd mice.

Striking inhibition of development of renal disease and prolongation of lifespan have been achieved in kdkd mice by restricting their daily food intake. Restricting protein intake alone did not prolong life nor did it inhibit development of kidney disease. The kdkd nephronophthisis, although very different histologically from the renal disease of B/W mice, may also have immunological components. Like the immunologically based renal disease of B/W mice, renal disease in kdkd mice is decreased or eliminated histologically by dietary restriction, which inhibits development of autoimmunity directed toward the erythrocytes of these mice. Further analysis will be needed to elucidate the cause of progressive renal disease in both the kdkd and B/W models and to permit understanding of the profound influence of restriction of food intake on development and progression of these very different renal diseases.

Animals↗
PubMed1978

Double ventricular response to an extrastimulus in a patient with triple atrioventricular pathways.

A patient with the Wolff-Parkinson-White Syndrome was studied through recordings of the intracardiac potentials and programmed atrial stimulation. During programmed atrial stimulation at progressively shorter coupling intervals (A1-A2 intervals), the His deflection was always recorded after the ventricular complex. Thus, at coupling intervals between 295 and 250 msec, there was a double ventricular response, one through the accessory pathway (QRS complex of the Wolff-Parkinson-White morphologic pattern) and the other by the normal atrioventricular pathway (normal QRS complex or with pattern of left bundle-branch block). At a coupling interval of 295 msec, the atrio-His (A-H) interval increased from 200 to 350 msec. This fact and the presence of two distinct A2-H2 intervals are suggestive of the existence of dual atrioventricular pathways, coexisting functionally with a lateral accessory bypass (Kent's bundle).

Bundle of His↗
PubMed1977

Late hexokinase activity expression during Bufo bufo development.

Hexokinase activity was detected in cytosols and homogenates from different developmental stages of Bufo bufo embryos starting from stage 17. Free glucose was measured in the embryo cytosol and was detected at each stage tested. At stage 15, a large increase of glucose content of the embryo cytosol occurs. Hexokinase expression in the embryo thus occurs after the increase of cytosol glucose content occurring at stage 15. The findings rule out that glucose by itself is the hexokinase inducer in vivo. The very low glucose utilization found by many authors during early amphibian development may be related to the late hexokinase expression during Bufo bufo development.

Animals↗
PubMed1976

Specific activities of 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase and glucose-6-phosphate isomerase during Bufo bufo development.

It has been suggested by some authors that during amphibian development, due to the higher glucose-6-phosphate dehydrogenase (EC 1.1.1.49) activity compared to that of 6-phosphogluconate dehydrogenase (EC 1.1.1.43), 6-phosphogluconate could accumulate in the embryo tissues and regulate the channelling of glucose-6-phosphate into glycolysis. Here, on the base of the specific activities of glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and glucose-6-phosphate isomerase (EC 5.3.1.9) found in the embryos of Bufo bufo during development, it is discussed whether 6-phosphogluconate can accumulate and play a regulative role on glucose-6-phosphate metabolism in the anuran embryo.

Animals↗
PubMed1976

A bacteriological study of the intestinal mucosa and luminal fluid of adults with acute diarrhoea.

Bacteriological studies of jejunal mucosal biopsy specimens and contents were performed on 22 hospitalized adult patients with acute diarrhoea and 24 control normal subjects. None of the washed homogenates of the mucosal specimens were sterile and only one fluid specimen obtained from a control subject was sterile. A definite enteric pathogen was found in only five of the 22 diarrhoea patients. There was no qualitative difference in the bacterial profile of the jejunal mucosa and contents of the diarrhoea patients from that of the control subjects, but there were significant quantitative differences for some bacterial categories. In the control as well as diarrhoea subjects, there was no qualitative difference in the bacterial profile of the jejunal mucosa from that of the fluid, but there were significant quantitative differences for some bacterial categories. The significance of the findings is discussed.

Acute Disease↗
PubMed1976

Dehiscence and active spore release in pathogenic strains of the yeast Metschnikowia bicuspidata var. australis: possible predatory implication.

Strains of Metschnikowia bicuspidata var. australis, pathogenic to brine shrimp (Artemia salina), were observed to form asci which, upon reaching maturity, forcibly expelled their needle-shaped spores. The mechanical force responsible apparently originates from the formation of an ectoplasmic mucilage capable of exerting pressure over all of the ascus contents; when the apex of the peduncle ruptures, the ascospores are violently released. Cytochemical analyses indicated that the gel is a substance highly resistant to chemical and enzymatic hydrolysis. Its chemical nature is not known as yet. The morphogenetic events of this process are described, and its ecological implication, the possibility of active mechanical predation in yeast, is discussed.

Aldehyde-Lyases↗
PubMed1973

Electron Micrography of Bud Formation in Metschnikowia krissii.

The fine structure of bud formation of Metschnikowia krissii was studied by means of ultramicrotomy and transmission electron microscopy. Bud protrusion and development were observed by scanning electron microscopy. Bud formation in this yeast takes place by an extension of a small localized area of the existing parent wall. The parent cell and its bud are initially separated by the plasmalemma, creating an intercellular site within which the generation of new cell wall (bud and birth scar areas) occurs centripetally. When the dividing wall is complete and new cell wall material is formed, a narrow cleavage plane becomes increasingly defined. This cleavage plane apparently proceeds laterally toward the direction of the existing outer walls which rupture, resulting in the separation of the bud from the parent cell. The bud scar is prominently convex in shape; the birth scar is less conspicuous and initially concave in shape. Comparison of bud formation in M. krissii is made with that observed in Saccharomyces cerevisiae and Rhodotorula glutinis.

Journal Article↗
PubMed1973

Electron micrography study of the asci and ascospores of Metschnikowia Kamienski.

Internal and surface structures of asci and ascospores were studied by transmission electron microscopy (TEM) and by scanning electron microscopy (SEM) to establish the character and number of ascospores within the ascus of Metschnikowia krissii. Enzyme digestion with snail gut enzymes and SEM examination suggested the presence of a single ascospore enclosed in a thick sheath of epiplasmic materials. Two closely associated ascospores without an epiplasmic sheath were clearly distinguishable from asci of M. bicuspidata var. chathamia when similarly treated. Ultramicrotomy and TEM established conclusively that M. krissii produced a single ascospore per ascus. Neither SEM nor TEM revealed any morphological detail of the ascospores of taxonomic significance.

Ascomycota↗