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Biomedical subjects

M Minami

Publications and source records attributed to M Minami.

At least 667 records · Page 37Linked to original sources
PubMed1978

Inhibition by potassium ion of the pregerminative response to L-alanine of unactivated spores of Bacillus cereus T.

The effect of potassium ion on L-alanine-inosine-induced germination of unactivated spores of Bacillus cereus T was studied. Unactivated spores germinated in 0.1 M sodium phosphate buffer (NaPB), but not 0.1 M potassium phosphate buffer (KPB), at pH 8.0 and at 30 C. Inhibition of germination was also observed on incubation of unactivated spores in NaPB containing potassium chloride. Previously it was demonstrated that germination of unactivated spores involves at least two steps, one induced by L-alanine, and the other by inosine. Potassium ion seems to inhibit the response of the spores to inosine, because: (1) Spores that had been preincubated with L-alanine in NaPB or KPB, germinated in NaPB but not KPB in the presence of inosine. (2) During germination in NaPB, incorporation of L-[14C]alanine showed bimodal kinetics with a rapid first phase and a second continuous phase, but in KPB the second phase of incorporation did not occur. The events occurring before germination of unactivated spores are discussed with reference to the initiation of germination.

Alanine↗
PubMed1978

[Effect of furosemide on ouabain toxicity in guinea pigs (author's transl)].

An attempt was made to study the effect of furosemide on the toxic and lethal dose (LD) of ouabain. Ouabain was infused continuously in guinea pigs anesthetized with urethane. The toxic dose of ouabain was determined by the minimal dose which produced ventricular arrhythmias. The LD of ouabain was 300.8 +/- 13.8 microgram/kg (mean +/- SE) in control guinea pigs. When furosemide was administered in a dose of 2 mg/kg i.v. prior to ouabain, this was significantly decreased to 225.2 +/- 14.1 microgram/kg (P less than 0.01). Furosemide-treated guinea pigs also showed a marked decrease in toxic dose of ouabain. In another series of experiment, the ouabain uptake by the heart and its subcellular fraction were determined by the infusion of 3H-ouabain at a constant rate under the same experimental conditions aforementioned. Approximately 30% decrease in the LD of 3H-ouabain was also observed in the furosemide pretreated guinea pigs. 3H-ouabain concentration in blood, cardiac muscles and microsomal fraction were lower than control group. These findings suggest that potentiation of ouabain toxicity produced by furosemide is not associated with increased cardiac ouabain uptake, but with increased sensitivity at the site of action.

Animals↗
PubMed1977

Hemoglobin Pyrgos (beta83 Gly replaced by Asp) in a Japanese family.

A screening survey for abnormal hemoglobins at a hospital in Mizunami city, Gifu prefecture, Japan detected a fast-moving variant of hemoglobin in a family of Japanese origin. The abnormal hemoglobin constitutes about 45 percent of the total hemoglobin from the propositus and another carrier in the family, but neither of these persons had anemia, jaundice, cyanosis or splenomegaly. Structural analysis of this hemoglobin revealed that the amino acid substitution was at residue 83 in the beta chain, where a glycine was replaced by an aspartic acid. This hemoglobin variant has been previously reported in a Greek child (hemoglobin Pyrgos) (1). Oxygen affinity of hemoglobin Pyrgos was found to be normal.

Aged↗