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Biomedical subjects

M Minami

Publications and source records attributed to M Minami.

At least 523 records · Page 29Linked to original sources
PubMed1989

Effect of benzene and its metabolites on natural killer activity of mouse spleen cells in vitro.

The effects of benzene and its metabolites, phenol and hydroquinone, on natural killer (NK) activity in mouse spleen cells in vitro were studied. NK activity was evaluated by the specific release percentage of 51Cr from labeled YAC-1 cells after YAC-1 cells (target cells) were incubated with spleen cells (effector cells) of mice at ratios of effector cells to target cells (E/T) of 100/1, 50/1, and 25/1. Benzene was shown to inhibit NK activity at concentrations of 1 x 10(-5) M-5 x 10(-5) M, phenol at concentrations of 1 x 10(-7)-5 x 10(-5) M and hydroquinone at concentrations of 1 x 10(-6) M-1 x 10(5) M. Phenol and hydroquinone depressed NK activity in a dose-dependent manner. This study demonstrated that NK cell function of mouse spleen was depressed by exposure to benzene and its metabolites in vitro. Hydroquinone and phenol have very potent toxicity for suppression of immunosurveillance.

Animals↗
PubMed1989

Effect of bunazosin on cardiac sympathetic nerve activity in anesthetized rats.

The present study was undertaken to elucidate the effect of selective alpha-1 adrenoceptor antagonist, bunazosin, on sympathetic nerve activity in anesthetized rats. Intravenous administration of bunazosin (10 micrograms/kg) produced a reduction of mean arterial pressure with a significant decrease in heart rate. Accompanied with its hypotension and bradycardia, bunazosin caused a decrease in inferior cardiac nerve activity. On the other hand, renal nerve activity, which reflects vasoconstriction, was not significantly decreased by bunazosin. These findings suggest that bunazosin may possess an anti-tachycardic action resulting from the reduction of cardiac sympathetic nerve activity and that the sympathoinhibitory action of bunazosin may not contribute to its hypotensive action.

Anesthesia↗
PubMed1989

[Serum and tissue concentration of tegafur and 5-FU after administration of tegafur suppository in patients with lung cancer--correcting the influence of residual blood in the tissue].

Tissue concentration of tegafur and 5-FU was studied in 25 patients with of primary lung cancer, given 2 x 750 mg of tegafur suppository daily preoperatively (total doses 3.75-9.75 g, mean 5.16 g). Furthermore, the influence of blood remaining in the tissue was corrected in the determination of tegafur and 5-FU concentration. The tegafur level in tumor tissue (9.614 +/- 5.739 micrograms/g) was significantly (p less than 0.01) low compared with those in serum and normal lung tissue (13.185 +/- 8.198 micrograms/ml, 12.954 +/- 10.048 micrograms/g). On the other hand, the 5-FU level in tumor tissue (0.124 +/- 0.208) was significantly (p less than 0.01, p less than 0.05) high compared with those in serum and normal lung tissue (0.019 +/- 0.013 micrograms/ml, 0.035 +/- 0.031 micrograms/g) and showed approximately 2.5 times more minimum effective concentration against tumor cells (0.05 micrograms/g). The results show that preoperative administration of 2 x 750 mg of tegafur suppository daily is effective for the transfer of tegafur and 5-FU to lung cancer tissue.

Adult↗
PubMed1989

Molecular cloning and expression of human leukotriene A4 hydrolase cDNA.

We have isolated a near full-length cDNA encoding human leukotriene A4 (LTA4) hydrolase from a human spleen cDNA library. The mature form of the enzyme consists of 610 amino acid residues and its molecular weight is calculated to be 69,153. The cDNA was inserted into an expression vector (pUC 9) and expressed in Escherichia coli. The fusion protein (EX85) possessed the LTA4 hydrolase activity with kinetic properties similar to the native enzyme.

Amino Acid Sequence↗
PubMed1989

[Complication related to operative procedure in lung cancer and mediastinal malignancy--report of 6 cases].

Of the patients who underwent surgical treatment for the respiratory system at our hospital over the past 9 years, 6 were postoperatively complicated with chylothorax, 1 with liquorrhea and the other one with paraplegia. Chylothorax occurred after mediastinal lymph node dissection which was carried out for the treatment of malignant tumors. In five cases, it occurred on the left side, and in the sixth case, it occurred on the right side. In 2 patients who received conservative treatment, there was no reduction in chyle outflow, and they died of cerebral infarction and sepsis. The other 4 cases were surgically treated. In 3 of them, the impaired site of the thoracic duct was confirmed by administration of Sudan III before surgery. We confirmed that early reoperation for the chylothorax after lung resection should be performed. Liquorrhea occurred from the 5th costvertebral joint which had been directly infiltrated by lung carcinoma. Fortunately, the postoperative course was uneventful, though the patient complained of dizziness and headache until 14 postoperative days. The case of paraplegia was caused by oxydized cellulose cotton that entered the epidural space via the intervertebral foramen. It was used for hemostasis in the 5th costvertebral joint. This case indicates that oxydized cellulose cotton, which swells when it absorbs water, should be carefully used for hemostasis around the nerves.

Adolescent↗
PubMed1989

[Palmar luxation of the metacarpophalangeal joint. Report of a case and review of the literature].

A palmar dislocation of the metacarpophalangeal (MCP) joint of a little finger occurred in a 25-year-old man and was successfully treated by an open reduction. Only seven other cases were reported in the literature. The mechanism of the palmar MCP joint dislocation was assumed to occur by hyperflexion injury. The dorsal capsule of the MCP joint was thought the essential pathologic feature that prevented successful closed reduction. The mechanism and pathologic anatomy of the palmar dislocation of the MCP joint is discussed.

Adult↗
PubMed1988

Modality-specific antinociception produced by intrathecal injection of anti-somatostatin antiserum in rats.

An intrathecal injection of antiserum against somatostatin significantly inhibited the nociceptive response to thermal but not mechanical stimulation in non-arthritic and adjuvant arthritic rats. The thermal antinociception induced by intrathecally injected anti-somatostatin antiserum was more remarkable in adjuvant arthritic rats than in non-arthritic ones. These results suggest that endogenous somatostatin participates in transmission of thermal nociception in the rat spinal dorsal horn in non-arthritic and arthritic conditions.

Animals↗
PubMed1988

A role of HLA-DQ molecules of stimulator-adherent cells in the regulation of human autologous mixed lymphocyte reaction.

In this study, we have found that treatment of stimulator autologous adherent cells with anti-HLA-DQ mAb resulted in markedly enhanced proliferative response of T cells in human autologous mixed lymphocyte reaction system wherein T cells were cultured with autologous adherent cells at near ratio of adherent cells to T cells in peripheral blood, in which T cells minimally proliferate. However, treatment of stimulator-adherent cells with anti-HLA class I, anti-DR and anti-DP mAb had no effect on the proliferative response of T cells under the condition. It was further observed that CD4-enriched cells could significantly proliferate in the presence of autologous adherent cells either untreated or treated with anti-DQ mAb, although treatment of adherent cells with anti-DR mAb blocked proliferative response of CD4-enriched cells. Moreover, the proliferative response of CD4-enriched cells was suppressed by addition of CD8-enriched cells in the presence of untreated adherent cells, whereas the proliferative response of CD4-enriched cells could not be suppressed by CD8-enriched cells when the adherent cells in the culture were treated with anti-DQ mAb. These observations suggest that CD8 T cells are required for suppression of proliferative response of CD4 T cells to HLA-DR molecules on autologous stimulator-adherent cells, and that HLA-DQ molecules on the surface of adherent cells play an important role in the induction of suppression by CD8 T cells.

Antibodies, Monoclonal↗
PubMed1988

Antinociception induced in rats by intrathecal administration of antiserum against calcitonin gene-related peptide.

To determine whether or not endogenous calcitonin gene-related peptide (CGRP) participates in pain transmission in the spinal dorsal horn, effects of an intrathecal injection of anti-CGRP antiserum on nociceptive threshold in the paw-pressure test was examined in non-arthritic and adjuvant arthritic rats. An intrathecal injection of the antiserum increased the nociceptive threshold in non-arthritic animals, while 0.9% saline, pre-immune serum and antiserum, previously absorbed by synthetic CGRP, were without effect. Adjuvant arthritic rats showed a hyperalgesia, and improvement occurred with intrathecal injection of the antiserum. Saline and absorbed antiserum were without effect on the hyperalgesia. These results suggest that the endogenous CGRP present in primary afferents probably has a facilitating function in nociceptive transmission in the spinal dorsal horn.

Animals↗
PubMed1988

Functional analysis of cloned macrophage hybridomas. VI. Differential ability to induce immunity or suppression.

We previously screened a series of macrophage hybridomas derived from fusion of P388D1 (H-2d) tumor cells with CKB (H-2k) splenic adherent cells for their ability to induce I-J restricted Ts cell responses. One Ia+ macrophage clone (63) consistently induced Ag-specific, I-J-restricted Ts. To evaluate whether macrophage hybridoma 63 also induced delayed-type hypersensitivity (DTH) immunity, mice were immunized with hapten-coupled macrophage hybridoma cells. Hapten-coupled splenic adherent cells and control macrophage hybridomas induced significant primary DTH responses, whereas hapten-coupled macrophage 63 induced little or no immunity when injected into H-2 compatible hosts. However, macrophage hybridoma 63 specifically activated I-Ak, I-Ad, or I-Ed restricted T cell hybridomas/clones, in vitro in the presence of appropriate Ag. Three different strategies designed to eliminate suppressor cell activity were successfully used to demonstrate that hapten-coupled macrophage 63 could also induce in vivo immunity. First, after immunization with hapten-coupled macrophages, mice were treated with cyclophosphamide. Second, macrophage 63 was treated with anti-IJ idiotype antibody before 4-hydroxy-3-nitrophenyl acetyl hapten (NP) coupling. Finally, haptenated macrophages were injected into I-A compatible but I-J incompatible recipients. These protocols are known to inhibit the induction of Ts activity, thus these results indirectly suggest that there is stimultaneous generation of Ts activity in vivo. The latter hypothesis was tested in adoptive transfer experiments. Transfer of lymph node cells from NP-63 primed B10.BR (H-2k) mice induced immunity in naive 4R animals, whereas the same number of immune cells suppressed NP-induced DTH responses in 5R mice. The combined results indicate that a cloned macrophage line can activate both Th and Ts cells. Macrophages which induce Ts activity may be responsible for maintaining the balance of immunity vs suppression. The data support the hypothesis that IJ interacting molecules (IJ-IM) expressed on macrophages are critical for induction of suppressor cell activity.

Animals↗
PubMed1988

An HLA-D region restriction fragment associated with refractory Behçet's disease.

We examined the association between HLA-DQ and refractory Behçet's disease at the genomic DNA level. Using a restriction endonuclease Taq I and a DQ beta cDNA probe, we identified the 1.9 kb DQ beta restriction endonuclease fragment tightly associated with refractory Behçet's disease. This observation indicates that genomic HLA-DQ variations affect the development of refractory Behçet's disease.

Behcet Syndrome↗
PubMed1988

Expression of human leukotriene A4 hydrolase cDNA in Escherichia coli.

The cDNA clone encoding human leukotriene A4 hydrolase was inserted into a vector pUC9 and expressed in Escherichia coli as a fusion protein containing the first 10 amino acid residues derived from a vector. The leukotriene A4 hydrolase activity was recovered in the soluble fraction of the transformants. The purified enzyme showed kinetic properties similar to the native enzyme, including inactivation by the substrate and sulfhydryl-modifying reagents. The results demonstrate that a protein with an Mr of 70,000 was expressed in Escherichia coli with a full enzyme activity and structural fidelity. Acquisition of the expression system makes it feasible to elucidate the reaction mechanism of the enzyme.

Cloning, Molecular↗
PubMed1988

Effect of 7-(3-[4-(2,3-dimethylphenyl)piperazinyl]propoxy)- 2(1H)-quinolinone (OPC-4392), a newly synthesized agonist for presynaptic dopamine D2 receptor, on tyrosine hydroxylation in rat striatal slices.

The effects of a newly synthesized compound, 7-(3-[4-(2,3-dimethylphenyl)piperazinyl]propoxy)-2(1H)-quinolinone (OPC-4392), on tyrosine hydroxylation in situ and in vitro were studied using rat striatal slices and tyrosine hydroxylase (TH) purified from bovine adrenal medulla, respectively. OPC-4392 dose-dependently inhibited L-dihydroxyphenylalanine (DOPA) formation in rat striatal slices with IC50 values of about 10(-6) M. The inhibitory effect of OPC-4392 on in situ DOPA formation was dose-dependently reversed by addition of sulpiride, a dopamine D2 receptor antagonist, whereas no change was observed by addition of nomifensine (5 X 10(-6) M), a blocker of dopamine uptake. From in vitro experiment using purified TH, OPC-4392 affected neither the enzymatic activity nor the Km value for 6-methyl-5,6,7,8-tetrahydropterin (6MPH4). These results suggest that OPC-4392 impairs in situ DOPA formation by stimulating presynaptic dopamine D2 receptor as a dopamine agonist, and not by directly inhibiting the TH activity.

Animals↗
PubMed1988

Radially deviated type of thumb polydactyly.

In duplication of the thumb there is sometimes radial deviation of one or both components. Eight such hands have been studied. The findings are described and treatment discussed.

Child↗
PubMed1988

Limited wrist fusions.

Seventeen patients had wrist fusions done for diseases other than rheumatoid arthritis of the wrist and carpal bones. Arthrodesis was done at the radiocarpal joint in five wrists and at the midcarpal joint in 12. There were 12 men and five women. Ages at the time of operation averaged 42 years. Follow-up ranged from 6 months to 5 years and 5 months, and with an average of 1 year and 10 months. Overall postoperative results were excellent in five wrists, good in seven, fair in two, and poor in three. Wrists with the midcarpal fusion fared better than those wrists with the radiocarpal fusion. Complications included one pseudoarthrosis and one rupture of the flexor pollicis longus tendon. In one of the 17 wrists new osteoarthritic changes surrounding the arthrodesed joints occurred.

Adolescent↗
PubMed1988

Alumina ceramic prosthesis arthroplasty of the metacarpophalangeal joint in the rheumatoid hand. A 2-4-year follow-up study.

Swanson's finger implant is being widely used to improve deformity of the thumb and finger and to restore function in the rheumatoid hand. Breakage of the implant and implant synovitis have been the most troublesome complications. The authors developed an alumina ceramic finger prosthesis to lessen these complications. This prosthesis was used for the metacarpophalangeal joint in 5 cases of flexion deformity of the thumb and in 21 cases (82 digits) with ulnar drift deformity. These cases were followed for 24-62 months (average, 38 months). Postoperative extension of the thumb was limited to 18 degrees and flexion was 48 degrees, on average. Postoperative range of motion was 30 degrees. The average limitation of extension of all digits was 18 degrees, and the average flexion was 54.5 degrees. The average range of motion was 36.5 degrees. Flexion deformity of the metacarpophalangeal joint of the thumb disappeared after operation, and ulnar drift was reduced to less than 10 degrees deviation in 87.8%. No dislocation or fracture of the prosthesis has been found on roentgenologic examination during short-term follow-up study. This prosthesis is useful for reducing deformity of the thumb and the finger in the rheumatoid hand. Postoperative extension of the metacarpophalangeal joint, however, has been unsatisfactory. The design of the prosthesis should be improved so that the rotational center of the metacarpophalangeal joint is located palmarly.

Adult↗
PubMed1988

Mucin-hypersecreting intrahepatic biliary neoplasms.

The radiologic findings are described in six patients with "mucin-hypersecreting" intrahepatic biliary neoplasms (biliary cystadenocarcinoma or papillary cholangiocarcinoma). Massive amounts of mucin were confirmed in the dilated bile ducts at surgery or autopsy. Computed tomography (CT) and ultrasound (US) demonstrated severe dilatation of the intrahepatic bile ducts distal to the tumor in all patients and of extrahepatic ducts in five. On cholangiograms, large, amorphous filling defects caused by retention of mucin were found in the dilated ducts in three patients. The tumor was multilocular and cystic in five patients; in the other patient it was too small to be detected at CT or US. The presence of liver tumor (mostly multilocular and cystic), marked biliary dilatation distal to the tumor, and filling defects in the dilated bile ducts are important findings that indicate a mucin-hypersecreting intrahepatic biliary neoplasm.

Adenoma, Bile Duct↗