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Biomedical subjects

M Miller

Publications and source records attributed to M Miller.

At least 415 records · Page 23Linked to original sources
PubMed1994

[Comparative study of BCG vaccines: Polish, Danish and Japanese].

In a group of 1111 school children aged 12 years from two selected town in Poland (Rzeszów and Ostrołeka) a comparative study of three BCG vaccines was performed, the Polish, the Danish, and the Japanese. Tests for viability of the vaccines gave the following results: for the Polish vaccine 17680000 culturable particles in 1 mg; the Danish--12060000, and the Japanese--43540000 respectively Children eligible for vaccination were selected on the basis of prevaccination tuberculin testing with 2 TU tuberculin RT-23 with Tween 80. All the three vaccines gave high level of postvaccination tuberculin reactions measured after 10 weeks. In more than 30% of children these reactions were higher than 15 mm. The mean postvaccination reactions after the Polish vaccine was 14.2 mm (s.d.3.7), after the Danish--13.7 mm (s.d.3.9), and after the Japanese 14.9 mm(s.d.3.0). The difference between the Danish and the Japanese vaccines was statistically significant, but between the Polish and the other two vaccines was not. It was also formed that children with larger postvaccination tuberculin indurations had larger diameter of preview BCG scars.

Age Factors↗
PubMed1994

Platelet thrombospondin interactions with human high and low molecular weight kininogens.

Multifunctional proteins, e.g. high molecular weight kininogen (HK, 120 kDa) and the homotrimer, thrombospondin (TSP, 540 kDa), which have more than one domain on a single polypeptide chain, are particularly well-suited to be structural elements of extracellular matrices because of their ability to bind to several macromolecules. We now demonstrate that 125I-high molecular weight kininogen (HKa) cleaved by purified kallikrein forms a complex with purified intact platelet TSP (540 kDa). HK also complexed with a proteolytic fragment (450 kDa) of TSP, lacking its three identical heparin-binding domains (HBD, 30 kDa), but failed to bind to a more extensively proteolysed molecule (210 kDa) lacking the C-terminal globular domain indicating that the binding on TSP-450 kDa is confined to the C-terminus. The binding of HK to intact TSP and to its 450 kDa fragment was of high affinity (Kd = 17-52 nM), specific, concentration dependent and saturable. Furthermore, we found both forms of the light chain (LC) of HK (56 and 46 kDa) resulting from cleavage by plasma kallikrein bound to both intact TSP and HBD independent of the presence of calcium ions. However, neither the epitope recognized by monoclonal antibody (MAb) C11C1 on domain 5 nor the prekallikrein binding site on domain 6 are involved, suggesting that the intervening proline-rich region may be the site of interaction. The heavy chain (HC) of HK required ionized calcium to bind to intact TSP or its 450 kDa homotrimer fragment.(ABSTRACT TRUNCATED AT 250 WORDS)

Blood Platelets↗
PubMed1994

Pathogenicity of a bovine attaching effacing Escherichia coli isolate lacking Shiga-like toxins.

Escherichia coli isolate 7996-90, obtained from a calf with diarrhea, had negative results of tests for K-88, K-99, 987P, F41, CS31A, F1845, F165 or E. coli adherence factor adhesins and had negative results of tests for the toxins heat-labile, heat-stable A, heat-stable B, Shiga-like toxin (SLT)-I or SLT-II. Strain 7996-90 had localized adherence to HEp-2 cells, caused actin rearrangement in host cells to which it adhered, hybridized with the eaeA probe, and produced the 94-kd outer membrane protein associated with attaching effacing lesions. This isolate caused attaching effacing lesions in Caco-2 cell polar monolayers, rabbit intestinal loops, and the intestines of gnotobiotic pigs. The isolate belongs to serotype O26: NM and is considered a class-II attaching effacing enteropathogenic E coli. Until recent addition of more sensitive assays at veterinary diagnostic laboratories, isolates such as 7996-90 were not readily recognized as pathogens because they failed to fit into the enterohemorrhagic E coli group, members of which, be definition, produce SLT. The assays described can facilitate diagnosis of attaching effacing E coli infection when histologic evaluation is hampered by autolysis.

Animals↗
PubMed1994

Effectiveness of early therapy with corticosteroids in Stevens-Johnson syndrome: experience with 41 cases and a hypothesis regarding pathogenesis.

Evaluation of therapy for Stevens-Johnson syndrome was initiated as a retrospective analysis and then extended to a prospective series of patients treated with corticosteroids. This report extends the initial prospective study of patients with Stevens-Johnson syndrome treated with corticosteroids and evaluates the total series of 41 patients relative to outcome and the presumptive etiology. We propose that management of Stevens-Johnson syndrome requires corticosteroid therapy and that the survival of patients with Stevens-Johnson syndrome may depend on this therapy. No fatalities or adverse effects due to corticosteroids were noted. Stevens-Johnson syndrome due to a drug, a drug metabolite or viral infection may mimic a graft-versus-host reaction in which the patient rejects skin, mucous membrane, kidney or liver cells to which the drug, drug metabolite, or virus has bound. Corticosteroids suppress the inflammatory rejection until the activating agent has been eliminated.

Adolescent↗
PubMed1994

Laparoscopic intracorporeal harvest of jejunal tissue for autologous transplantation.

Our goal in this study was to assess the feasibility and safety of performing an intracorporeal laparoscopic jejunal harvest. The initial technique was developed and refined in a pig and then a dog model. In the animal studies, careful dissection of the jejunal flap with its feeding vessel was accomplished along with an intracorporeal anastomosis. The laparoscopic dissection was facilitated by temporarily anchoring the jejunal flap to the anterior abdominal wall and transilluminating the mesentery. We present the first case report of a patient who underwent a laparoscopic jejunal harvest, intracorporeal small bowel anastomosis, and a microvascular anastomosis in the neck for reconstruction of the laryngopharynx.

Adult↗
PubMed1994

Acute endocrine emergencies.

Certain endocrinologic disorders occur more frequently in the elderly than in younger patients. With the steady increase in the elderly population, acute endocrinologic disorders have become more common. Characteristics of elderly patients, such as diminished homeostatic reserve, increased medication use, and the presence of other diseases allow the transformation of symptoms into medical emergencies.

Acute Disease↗
PubMed1994

Addiction to surgery: a nursing dilemma.

Factitious disorder is either a rare or underdiagnosed disorder and therefore, not one the nursing staff anticipates. Once the disorder is diagnosed, the intense feelings of the nursing staff may overwhelm them. Therefore, the staff should be able to participate in discussion sessions conducted by a psychiatric clinical nurse specialist or another qualified person. Such sessions should focus on recognition of feelings, comparison of one's feelings with those of other staff members, and sharing of perceptions about the situation.

Adult↗
PubMed1993

A left-handed crossover involved in amidohydrolase catalysis. Crystal structure of Erwinia chrysanthemi L-asparaginase with bound L-aspartate.

The crystal structure of L-asparaginase from Erwinia chrysanthemi in the presence and absence of L-aspartate was determined at 1.8 A resolution. Conserved residues in a left-handed crossover (a rare occurrence in protein structures) link pairs of dimers into the catalytically active tetrameric form of the enzyme. The structure of ErA containing bound aspartic acid shows that this unusual strand connectivity is an essential part of the active site architecture, responsible for releasing the product of the enzymatic hydrolysis. The orientation of the bound aspartate indicates for the first time a threonine residue as a catalytic nucleophile.

Amidohydrolases↗