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Biomedical subjects

M Mikami

Publications and source records attributed to M Mikami.

At least 127 records · Page 7Linked to original sources
PubMed1984

Establishment of human monoclonal anti-DNA antibody producing cell lines.

We developed a useful method for the establishment of stable cell lines producing human monoclonal anti-DNA antibody by in vitro Epstein-Barr virus infection. The practical limitation for the cloning was overcome by 2 procedures. One was a microculture system using a small number of the culture. Another was enrichment of anti-DNA producing cells at an early stage and prior to the cloning. The combination of these procedures allowed ready derivation of the cell lines secreting monoclonal anti-DNA antibody. Sixteen cell lines were cloned by utilizing colony formation methods in soft agarose. About 14-32 micrograms per ml of IgM with specific antibody activity were obtained in the supernatant of the cells. The antibody reacted with double-stranded and/or single-stranded DNA. These cells have been continuously producing the specific antibody for more than 3 years. We may extend this procedure for obtaining other autoantibodies, such as anti-T cell antibodies.

Animals↗
PubMed1983

Effects of contact lens wear on mitosis of corneal epithelium and lactate content in aqueous humor of rabbit.

Various types of contact lenses, i.e., poly-methyl-methacrylate hard lenses, poly-2-hydroxyethyl-methacrylate soft lenses and two kinds of gas-permeable hard lenses with different permeabilities to oxygen, were placed on one eye of the albino rabbit for varying lengths of time. Using the fellow eye as the control, studies were carried out of the effects of the lens wear on the mitosis in the corneal epithelium and the lactate concentration in the aqueous humor. The contact lens wear suppressed the mitosis and increased the concentration of aqueous lactate, the degree of changes being greater as the permeability of the lens to oxygen decreased and the period of wear became longer.

Animals↗
PubMed1982

Multiple myeloma among atomic bomb survivors in Hiroshima and Nagasaki, 1950-76: relationship to radiation dose absorbed by marrow.

The relationship between atomic bomb exposure and the incidence of multiple myeloma has been examined in a fixed cohort of atomic bomb survivors and controls in the life-span study sample for Hiroshima and Nagasaki. From October 1950 to December 1976, 29 cases of multiple myeloma were confirmed in this sample. Our analysis shows that the standardized relative risk (RR) adjusted for city, sex, and age at the time of bombings (ATB) increased with marrow-absorbed radiation dose. The increased RR does not appear to differ between cities or sexes and is demonstrable only for those survivors whose age ATB was between 20 and 59 years. The estimated risk in these individuals is approximately 0.48 cases/million person-years/rad for bone marrow total dose. This excess risk did not become apparent in individuals receiving 50 rad or more in marrow total dose until 20 years or more after exposure.

Adult↗
PubMed1981

[An experimental study of the pedicle omental graft with microvascular anastomosis (author's transl)].

The principle of the management of chronic osteomyelitis consists of debridement of sequestra, scar tissues and sinuses, exposing fresh healthy bone and administration of appropriate antibiotics. Here arises the problem: how to eliminate the dead space upon closure of the wound. This paper reports the results or animal experiments on whether pedicle omental graft with microvascular anastomoses can be used to eliminate the dead space of bone. In the first experiment, autogenous omentum with a vascular pedicle was transplanted around the femur of dogs by microvascular anastomoses. Sixteen dogs were operated on and 4, 8, 12, and 20 weeks after the operation, 4 dogs of each stage were anesthetized and anastomosed artery of each was exposed. The contrast medium was injected from this site and then the dog was killed. Specimens from the dogs were histologically examined, and it was proved that the transplanted omentum was alive even 20 weeks after the operation and vascular connection between grafted omentum and the bone and its surrounding tissues had been established. In the second experiment, omentum was transplanted around the frozen necrotized femur of the dog by the same technique. In the control group, the femur of the dog was frozen but omentum was not transplanted. Eight, 12, 20, and 24 weeks after the operation, 3 dogs respectively were labeled by tetracycline and 2 weeks after that, the dogs were killed. Specimens from the femur were studied by fluorescence and light microscopy. As the result, the remodeling of the bone was faster in omental group than in control group. From these two experiments, the following conclusion was drawn. When omentum with vascular pedicle was transplanted around the femur of a dog by vascular anastomoses, it continued to live and makes rich vascular connection with surrounding tissues and as revascularization of necrotic bone accelerated, the remodeling of the bone was promoted. This phenomenon could be applied to the treatment for chronic osteomyelitis.

Animals↗