Search PubMed⌕ Search

Biomedical subjects

M Miegeville

Publications and source records attributed to M Miegeville.

33 records · Page 2Linked to original sources
PubMed1977

[Autoradiography of the exchanges that can occur between murine sarcoma cells (BP 8) and yeast cells (Saccharomyces cerevisiae, complete or protoplast)].

We can tell after observing the resulting negative that yeasts are able, in our experimental conditions, to collect a sequence of ADN and of ARN proceeding from the cancerous cells. Then, those yeasts could turn their synthesis towards the production of new materials. If those informed yeasts were introduced into a mouse, they would induce at the level of the immunocompetent cells a specific immunizing antitumoral power.

Animals↗
PubMedDate not supplied

[Deviation of the life cycle of Dipetalonema viteae (Filarioidea)].

Dipetalonema viteae is a filarial that can evoluate among hosts zoologically broadly apart (Ixodides and Argasides), but always gathered from meriones burrows. Its evolution is, on the contrary, blocked among most of the other ticks, particularly among Ornithodoros erraticus morphologically very similar to the normal vector. Our work concerns the experimental deviation of the cycle of Dipetalonema viteae, with its possible adaptability in the bosom of an intermediate of fowl tropism and a permanent host, different from a gnawing.

Animals↗
PubMedDate not supplied

[Release of "Saccharomyces cerevisiae" protoplasts: scanning electron microscope study (author's transl)].

In a previous study we described the minimal methodology used to obtain protoplasts from ascomycetous yeasts. Using a reducing agent associated with 1,3-beta-glucanase at 26 degrees C, protoplasts were invariably obtained. In the present study we localized the disruption spots of the cell wall using the two same reagents. The observations were made with the scanning electron microscope. The disruption site was always in the subterminal region, and this very simple structure (proteins with disulphide bridges and 1,3-beta-glucans) was opposite the birth-scar. The dissociation of the two reagents showed that a small part of the yeast population was able to release protoplasts with only glucanase. We believe these very sensitive yeasts (2 to 10% population) to be very young cells. These disruption sites seemed very different from budding-sites. They might be identical with elongation-sites, or with the opening in the ascus-wall during germinating ascospore release.

Glucan Endo-1,3-beta-D-Glucosidase↗
PubMedDate not supplied

[Studies in vivo and in vitro on relations between Schistosoma mansoni and some enterobacteria strains].

Studies performed in vivo and in vitro, using scanning electron microscopy, on relations between Schistosoma mansoni and some enterobacteria show a strong association for certain bacterial strains (Salmonella typhimurium). Salmonellae adhesion on tegumental surface of male and mature Schistosoma is only possible in vitro owing to bacterial synthesis of strong fibres penetrating inside the worm. These interactions are not found with Escherichia coli, Proteus mirabilis or with young schistosomula.

Adhesiveness↗
PubMedDate not supplied

[Attempts at demonstrating the Schistosoma-Salmonella relationship. In vivo and in vitro studies].

The optical microscopy (on histological preparations) and scanning observations of Schistosoma mansoni is conducted in the presence of various species of Salmonella. It is well-obtained that the Schistosoma maturity, its metabolic activity and the action of antibodies anti-salmonella have influenced the bacterian fixation on the worm. An hypothesis about the possible mechanism of this attachment is then developed and also an hypothesis about the protection of bacteria against antibiotherapy among patients infected with Schistosoma and carriers of salmonellosis.

Animals↗
PubMedDate not supplied

[New contribution on Dipetalonema viteae. Keeping adults alive in vitro. Scanning electron microscope study of the development of embryos and young larvae L1 and L2].

The adult stages of Litosomosoides carinii, Dirofilaria uniformis and Dirofilaria immitis have been successfully maintained in vitro though microfilaria production by the worms continued only for a period of one to 18 days. In this paper we describe the results obtained in a series of experiments in which adult L1 and L2 stages of Dipetalonema viteae were maintained in vitro.

Animals↗
PubMedDate not supplied

[Gilchrist's disease and fungal taxonomy: a thorny problem].

The study of iconographical data and of six american and african strains of B. dermatitidis allows us to conclude that there are several discriminating characters between the members of the Blastomyces dermatitidis complex. The african strains are distinguished by the lack of sexual forms and old strains show the lost of the M lead to Y conversion. An identical character prevails among the strains of the two continents: tuberculate conidia which are obtained on corn meal agar. The authors are insisting on the identical characters encountered on Blastomyces and Histoplasma. All the mycotic strains described from cases of Gilchrist disease possess a Chrysosporium period. The experimental Gilchrist disease performed on rodents is more often obtained with members of the genus Chrysosporium.

Africa↗