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Biomedical subjects

M McMillan

Publications and source records attributed to M McMillan.

90 records · Page 5Linked to original sources
PubMed1979

Magnification renal arteriography.

Magnification selective renal arteriograms were performed on 24 patients, 12 of whom were hypertensive, and compared with non-magnification arteriograms by two observers independently. The magnification angiograms were performed on a Siemens Microfocus Bi 125/3/50 RG tube with a 0.1 mm focal spot. Of the 24 patients examined, information crucial to the diagnosis was found only on the magnification films in three patients (12.5%). Extra information compared with the nonmagnification films was found in the magnification films in 12 patients (50%). No additional information was discovered in the remaining nine patients (37.5%). The magnification angiograms enabled the interlobular vessels to be visualized--this was not possible on the non-magnification films. Against the additional information gained must be weighed the disadvantages of magnification arteriography which include increased radiation dose and lengthening of procedure time plus additional injections of contrast. In conclusion, there is a place for magnification renal arteriography and the advantages seem to outweigh the disadvantages.

Adolescent↗
PubMed1979

An outbreak of suspected solanine poisoning in schoolboys: Examinations of criteria of solanine poisoning.

Seventy eight schoolboys became ill after eating potato at lunch on the second day of the autumn term. Seventeen of the boys required admission to hospital. The gastrointestinal, circulatory, neurological and dermatological findings and the results of laboratory investigations were in keeping with solanine poisoning. The illness affected the junior boys and all the monitors but no other senior boys or staff. This pattern was compatible with the consumption of a relatively small number of toxic potatoes believed to have come from a bag (A) left in the stores since the summer term. The amount of solanine in potato waste recovered after the meal was excessive as assessed by its anticholinesterase activity. The amount of alpha-solanine and alpha-chaconine in the flesh and peel of potatoes from a bag (B) known to have been left from the previous term was high. The anomalously narrow margin between the solanidine alkaloid content reported for normal and toxic potatoes might perhaps result from an excessive synthesis by the latter of additional, related steroids, such as sapogenins and saponins, which, by promoting gastrointestinal absorption or other means, might enhance the toxicity of solanidine alkaloid.

Adolescent↗
PubMed1977

Structure of murine Ia antigens: partial NH2-terminal amino acid sequences of products of the I-E or I-C subregion.

Partial amino acid sequence of the Ia molecule encoded by the I-E or I-C (I-EC) subregion of the major histocompatibility complex of the mouse are presented. The Ia molecule appears to be comprised of two noncovalently associated polypeptides. The larger subunit, alpha, has an approximate molecular weight of 35,000 and the smaller subunit, beta, an approximate molecular weight of 28,000. Several interesting homology relationships (or the lack thereof) are apparent when the Ia polypeptides from the I-EC subregion are compared both with their counterparts from man and guinea pig and with the molecules encoded in the I-A subregion. Clearly the most impressive homology relationship is that seen between the alpha polypeptide from the I-EC subregion of mouse and its human counterpart. This is in striking contrast to the beta polypeptide, which bears no apparent homology to its human counterpart.

Amino Acid Sequence↗
PubMed1970

Fine structure of an oat cell carcinoma of the lung associated with ectopic ACTH syndrome.

Electron-microscopic examination of an oat-cell carcinoma associated with the ectopic ACTH syndrome demonstrated characteristic cytoplasmic granules of 60-240 nm. diameter, consisting of a dense central core separated by a clear halo from an outer investing membrane. Comparison with previously examined non-secretory oat-cell carcinomas showed the granules to be more numerous in the present case. They are considered to represent secretory activity in the tumour.

Adrenocorticotropic Hormone↗
PubMed1960

THE IDENTIFICATION OF A FLUORESCENT REDUCING SUBSTANCE IN THE URINE OF PATIENTS WITH RHEUMATOID ARTHRITIS: THE EXCRETION OF 3-HYDROXYANTHRANILIC ACID IN THIS AND OTHER CONDITIONS.

The report that 2,5-dihydroxyphenylpyruvic acid occurs in the urine of patients with collagen disease has not been confirmed, and no evidence was found for regarding collagen disease as being due to an inherent error in tyrosine metabolism as suggested by Japanese workers.A strongly reducing substance was conspicuous in the urine of patients with rheumatoid arthritis but not in that of normal persons. This substance was identified as 3-hydroxyanthranilic acid (3-HAA).A method is described for the measurement of 3-HAA in urine, employing ether extraction, paper chromatography in a formate solvent, and visual assessment by fluorescence in ultra-violet light and colour with Ehrlich's reagent. Normal persons excreted from less than 125 to 375 mug./day (average rather lower than 200 mug./day); those suffering from rheumatoid arthritis excreted from less than 125 to 2,250 mug./day (average 820 mug./day). The excessive excretion of 3-HAA in persons with rheumatoid arthritis was investigated in terms of other aspects of the activity of the disease. Some relationship was suggested between excretion levels, the erythrocyte sedimentation rate, and the sensitized sheep cell test. Cases of osteoarthritis, other orthopaedic conditions, and miscellaneous diseases were examined. A few of the latter, including diseases of the haemopoietic system, were associated with increased excretion of 3-HAA.

Journal Article↗
PubMedDate not supplied

Expression of I-Ak class II genes in mouse L cells after DNA-mediated gene transfer.

The I-region of the mouse H-2 complex encodes I-region-associated or Ia antigens which are involved in lymphocyte interactions and in the regulation of immune responsiveness. Two Ia antigens, termed I-A and I-E, have been identified; both are cell-surface integral membrane glycoproteins which consist of two noncovalently linked polypeptide chains designated alpha (molecular weight (MW) approximately 34,000) and beta(approximately 28,000 MW). Their expression is restricted to B lymphocytes and to antigen-presenting cells such as macrophages. Inside the cell, these alpha beta dimers are associated noncovalently with a nonpolymorphic chain of 31,000 MW designated the invariant (Ii) chain. Cloning of 200 kilobases (kb) of the I-region from mice of the H-2d and H-2k haplotypes was accomplished recently in our laboratory, and the genes encoding the four polypeptide chains, A alpha, A beta, E alpha and E beta, were identified. We now describe the successful expression of I-Ak molecules in transfected mouse L cells.

Animals↗
PubMedDate not supplied

Isolation of the MHC genes encoding the tumour-specific class I antigens expressed on a murine fibrosarcoma.

The C3H UV-induced fibrosarcoma, 1591, is highly immunogenic and, therefore, is readily rejected when transplanted into immunocompetent syngeneic recipients. Previous analysis of 1591 with tumour-specific or H-2-reactive monoclonal antibodies revealed that this antigenicity might be due to the expression of two novel class I major histocompatibility complex (MHC) antigens. In this report we describe the molecular cloning and initial characterization of three genes which account for all of the unique serological class I reactivities observed on this tumour. These include two distinct, but highly conserved, H-2L-like genes, and a third gene the product of which bears determinants which are characteristic of both the tumour and of class I products of the H-2k haplotype. Moreover, each of these genes contains a polymorphic restriction enzyme fragment which is detected in the class I sequences of 1591 relative to normal C3H tissue. Since the expression of these polymorphic class I sequences is relevant to the immunogenicity of 1591, the mutational events by which these genes were generated may be significant to the immunobiology of this tumour.

Animals↗
PubMedDate not supplied

Evidence that derivation of the adenocarcinoma LT85 predated establishment of the H-2km2 mutation in a C3Hf colony of mice.

The adenocarcinoma LT85 was chemically induced in a mouse from a C3Hf colony shown subsequently to be inbred for a gene conversion-like mutation at the H-2K locus, characterized by a clustered four nucleotide substitution in exon 3. The H-2K phenotype of LT85, however, more closely resembles that of C3H rather than the mutant strain now designated C3Hf H-2km2. We cloned and sequenced the H-2K gene from this tumor to determine whether (i) LT85 might carry a tumor-associated somatic reversion of the H-2Kkm2 germline mutation of (ii) the tumor was induced in a mouse that was genetically H-2k rather than H-2km2. Our analysis confirmed that the LT85 H-2K allele is identical throughout the entire coding region to C3H H-2Kk. To exclude the possibility of a somatic reversion by recombination, we used an oligonucleotide probe corresponding to the region altered in H-2k to show that the C3Hf genome lacks the necessary coding information to reverse the H-2Kkm2 mutation through a sequence exchange with another class I locus. Since it is unlikely that multiple independent point mutations would account for restoration of this stretch of H-2Kk sequences, the tumor was probably established in a mouse carrying a normal H-2Kk allele, possibly at a point prior to the establishment of the H-2Kkm2 mutation as a homozygous trait within the C3Hf colony.

Adenocarcinoma↗
PubMedDate not supplied

Condom use on campus.

Explore the source record for details and available documents.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Tuberculosis among foreign-born residents of southern Florida, 1995.

OBJECTIVE: To examine the characteristics of foreign-born people with tuberculosis (TB) in Southern Florida, their contribution to the total number of TB cases, and available data on their HIV status as well as to determine the number of cases detected by the overseas medical screening of immigrants and refugees. METHODS: The authors reviewed TB cases reported by Broward, Dade, and Palm Beach counties in 1995. Case records were matched against the CDC Division of Quarantine database of immigrants and refugees suspected to have TB at the time of visa application overseas. RESULTS: Nearly half (49%) of TB cases in the three counties were among people born outside the United States--34% in Broward County, 58% in Dade County, and 40% in Palm Beach County. A high percentage (26%) were co-infected with HIV. Of those with known date of arrival, 68% had been in the United States for five or more years. Only three cases had been identified by overseas immigrant screening. CONCLUSIONS: A low percentage of TB cases in foreign-born people were identified through the overseas screening system. Controlling TB in South Florida will require efforts targeted toward high risk populations, including people with HIV infection.

Adult↗