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Biomedical subjects

M McClenaghan

Publications and source records attributed to M McClenaghan.

24 records · Page 2Linked to original sources
PubMed1988

Distribution of plasmid sequences in avian and mammalian strains of Chlamydia psittaci.

Plasmid DNA from an avian strain of Chlamydia psittaci was purified and estimated to be 7.9 kb in size using restriction endonuclease analysis. A 5.9 kb fragment of this plasmid was cloned, mapped and used to screen a range of chlamydial strains. Hybridizing DNA was absent from ovine abortion and arthritis isolates and also from the Cal 10 strain but related sequences were detected in C. psittaci strains of feline pneumonitis, guinea-pig inclusion conjunctivitis, ovine conjunctivitis and C. trachomatis serovar L2. The plasmid DNA from the feline strain was shown to have a distinct restriction endonuclease profile. Similar plasmid sequences were detected in all avian isolates tested: thus the clone may have a useful diagnostic role for the detection of the pathogen in its natural host and in zoonotic episodes.

Animals↗
PubMed1984

Comparison of Chlamydia psittaci isolates by DNA restriction endonuclease analysis.

Preparations of DNA from 12 Chlamydia psittaci isolates and one Chlamydia trachomatis strain were compared by restriction endonuclease analysis. Polyacrylamide gel electrophoresis, followed by silver staining, resulted in optimal resolution of fragments generated by digestion. By this technique, four distinct electropherotypes were demonstrated when ovine abortion, ovine arthritis, and avian and Cal10 strains of C. psittaci were examined. Minor profile differences allowed the discrimination of avian isolates derived from psittacine and columbiforme species, and the Cal10 DNA electropherotype was shown to have features in common with these profiles. However, there were no detectable differences in the DNA patterns of eight ovine abortion isolates.

Abortion, Septic↗
PubMed1982

Detection of mixed Mycoplasma species.

Immunofluorescence can be used to demonstrate mixed species within a mycoplasma culture; however, it may fail to do so if one species is present in very low numbers. To enhance the detection of minor components in such mixtures, a technique was developed, based on the growth inhibition test, whereby the growth of the major component in a mixed culture was inhibited, thus permitting the minor component to emerge. The method was applied to 67 field isolates from chickens and turkeys, which had been examined by immunofluorescence and were thought to contain only one mycoplasma species. Of these, 26 cultures were found to be mixtures, and in some instances the pathogenic species Mycoplasma gallisepticum and M. meleagridis were revealed.

Animals↗
PubMedDate not supplied

Alteration of the quality of milk by expression of sheep beta-lactoglobulin in transgenic mice.

Milk contains a large amount of protein, most of which consists of a few major species synthesized in the mammary gland. The genes encoding these proteins are single-copy, and expressed during pregnancy and lactation. Although beta-lactoglobulin (BLG) is the major protein in the whey of ruminants, it is not present in rodent milk. We have generated transgenic mice carrying the sheep BLG gene, and show that in such mice, BLG is specifically and abundantly expressed in the mammary gland during lactation. This results in a remarkable alteration of milk composition. These findings suggest that the manipulation of milk composition by gene transfer has considerable potential for the improvement of dairy animals.

Animals↗