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Biomedical subjects

M Mayumi

Publications and source records attributed to M Mayumi.

At least 343 records · Page 19Linked to original sources
PubMed1975

Hepatitis B surface antigen in the serum of infants after deliver from asymptomatic carrier mothers.

A survey of sera of 5,993 pregnant women for hepatitis B surface antigen in Tokyo revealed 139 asymptomatic carriers (2.3%), essentially the same as that of a control population of comparable age group (2.2%). None of 59 specimens of cord blood of their newborn infants was positive for HBsAg according to an immune adherence hemagglutination assay. In 11 mother-child pairs in whom follow-up was possible for more than seven months, HBsAg was detected in the sera of eight infants within the first six months, after birth, with antigenemia persisting throughout the observation period, while antigenemia was not detected in the remaining three. The subtype of HBsAg was identical for each mother-child pair. The antigen was detected in sera of two of the infants after appropriate incubation periods of 123 and 133 days, respectively, whereas in others it was detected as early as 5 and 13 days after delivery.

Antigens, Viral↗
PubMed1975

South-to-north gradient in distribution of the r determinant of hepatitis B surface antigen in Japan.

A total of 2.305 sera positive for hepatitis B surface antigen were obtained from asymptomatic carriers throughout Japan and subtyped for the d, y, w, and r specificities by the hemagglutination inhibition method. Determinant d prevailed in Japan (d, 99.1%; y, 0.9%), and there was no regional variation in its occurrence. In sharp contrast, there was a marked variation in the distribution of the w and r determinants, forming an apparent south-to-north gradient of r. The percentage of r determinant was highest in Kyushu (92%-94%); it decreased gradually along the axis of Japan to the north and was lowest in Akita (46%), which is located at the north end of Honshu.

Alleles↗
PubMed1975

Determination of antibody to hepatitis B core antigen by means of immune adherence hemagglutination.

A simple and rapid method utilizing immune adherence hemagglutination has been developed for the detection of antibodies to hepatitis B core antigen (anti-HBc). Hepatitis B core antigen (HBcAG) was prepared from Dane particles that had been isolated from plasma of asymptomatic antigen carriers. The method was specific and about 10 times more sensitive than the conventional complement-fixation method. A total of 215 serum samples obtained from healthy blood donors were surveyed for HBsAG and anti-HEc, as well as for hepatitis B surface antigen (HBsAg) and antibody to HBsAG (anti-HBs). Anti-HBc was found in 36 serum samples, at a prevalence rate higher than that of anti-HBs (31/215)...

Animals↗
PubMed1975

Studies on the role of proteases in the biochemical mechanisms of tissue injury.

A complex of inflammatory protease with an inhibitor was found to be present in the early phase of Arthus skin lesions in guinea pig. From the extract of inflamed lesions a complex was partially purified by chromatography on DEAE-Sephadex A-50 and GE-cellulose, in that order. The inhibitor was finally dissociated rom the protease complex by treating them with cysteine followed by Sephadex G-50 chromatography. Active protease was recovered as a single peak which also contained some inhibitor activity. The isolated inhibitor was capable of inactivating inflammatory protease as well as papain. It was electrophoretically homogenous and was identified as protein or polypeptide in the serum alpha-1-globulin fraction. The significant role of the inhibitor in regulating the acute phase of inflammatory process is emphasized.

Animals↗
PubMed1975

Application of the paired label radioantibody technique to detection and subtyping of hepatitis B surface antigen.

A sensitive radioimmunoassay has been developed for the detection and subtyping, in regard to w and r specificities, of hepatitis B surface antigens (HBsAg). The binding of monospecific rabbit antibodies directed to either w or r determinant, labeled with 131I and 125I, respectively, by HBsAg was determined in the same experiment, taking advantage of the fact that these specificities are in general, expressed mutually exclusively. HBsAg in the test sample were bound to solid phase guinea pig anti-HBsAg antibody tube before the test. The ratio of percentage uptake of 125I anti-r to that of 131I anti-w for 28 normal sera without antigen was 0.69 0.18. The ratio for seven sera containing HBsAg with w determinant was less than 0.1, and that for 34 with r determinant was more than 10; 100-fold difference was noted between values of w and r antigens.

Animals↗