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Biomedical subjects

M Matsuda

Publications and source records attributed to M Matsuda.

At least 1,279 records · Page 71Linked to original sources
PubMed1983

Transient abnormal myelopoiesis followed by acute megakaryoblastic leukemia with extramedullary tumors. An autopsied case of Down's syndrome.

An autopsied case of acute megakaryoblastic leukemia is described in a 20 months old, Japanese female infant with Down's syndrome. She presented spontaneous remission of transient abnormal myelopoiesis in her neonatal period, which was followed by acute megakaryoblastic leukemia 1 year later. The clinical picture of acute megakaryoblastic leukemia was initially characterized by peripheral pancytopenia with a few blasts, the absence of hepatosplenomegaly, but ended in overt leukemia characterized by increase of blasts, marked hepatosplenomegaly, and elevated LDH. She died 6 months after the onset at 20 months of age. Autopsy findings revealed widespread leukemic infiltration comprised of megakaryoblasts and megakaryocytes, and extramedullary tumors in the left tibia, the liver, both kidneys, and the endocardium of the heart. Identification of the megakaryocytic cell line was performed in immunohistochemistry and electron microscopy. Chromosomal analyses of peripheral blood disclosed 47, XX, +21, in her neonatal period but disclosed 48, XX, +G, +G, in acute megakaryoblastic leukemia.

Acute Disease↗
PubMed1983

4-Aminobutyraldehyde as a substance convertible in vivo to GABA.

[2,3-3H]4-Aminobutyraldehyde ([3H]ABAL) was injected subcutaneously into mice, which were sacrificed at various intervals following injection. [3H] gamma-Aminobutyric acid ([3H]GABA) synthesized in vivo from [3H]ABAL was extracted from the brains, separated, and quantitated. The results showed that in the brain, injected [3HABAL was rapidly transformed into [3H]GABA. [3H]ABAL may penetrate the blood--brain barrier into the central nervous system and then be oxidized to [3H]GABA.

Aldehydes↗
PubMed1983

Effect of exercise on left ventricular diastolic filling in athletes and nonathletes.

To evaluate the effect of exercise on left ventricular diastolic filling, the following were measured at rest and during exercise in 14 control subjects and 15 athletes, using digitized M-mode echocardiography: the peak early diastolic lengthening rate of the left ventricular dimension and the filling volume and the filling fraction during the first 0.10 s of diastole. During ergometer exercise performed at a level that increased the heart rate to 100 beats/min, there were significant increases in the peak normalized lengthening rate of the left ventricular dimension (control subjects, 4.2 +/- 1.3 vs. 6.1 +/- 1.1 s-1, mean +/- SD, P less than 0.001; athletes, 5.3 +/- 0.9 vs. 7.4 +/- 1.1 s-1, P less than 0.001), filling volume (control subjects, 15 +/- 12 vs. 33 +/- 10 ml, P less than 0.001; athletes, 21 +/- 12 vs. 63 +/- 18 ml, P less than 0.001), and filling fraction (control subjects, 21 +/- 14 vs. 42 +/- 17%, P less than 0.005; athletes, 21 +/- 13 vs. 54 +/- 12%, P less than 0.01). The peak lengthening rate of the left ventricular dimension, the filling volume, and the filling fraction were significantly greater in athletes than in control subjects during exercise (P less than 0.005, P less than 0.001, and P less than 0.05, respectively). Augmented early diastolic filling may be a mechanism to provide adequate filling for the ventricle at high heart rates produced by exercise, especially in athletes.

Adolescent↗
PubMed1983

Factor XIII deficiency associated with Klippel-Weber disease, platelet dysfunction and cryofibrinogenemia.

A 23-year-old woman with factor XIII deficiency was presented. The patient had no consanguinity, but familial traits were present. A bleeding tendency and poor wound healing had been noted in the patient since birth. She had hemangiomas in the leg and vulva (Klippel-Weber disease). Hematologic studies revealed platelet dysfunction, cryofibrinogenemia and mild chronic disseminated intravascular coagulation with prolonged PT and PTT, hypofibrinogenemia, a high turnover rate of 125I-fibrinogen and mild elevation of fibrinogen-fibrin degradation products, beta-thromboglobulin and platelet factor 4. A decrease in clot retraction and a marked reduction in maximal amplitude of thrombelastogram were also found. The assay of the factor XIII level was 10% by the antiserum inhibition method, and the assay of subunits A and S were 16 and 29%, respectively, by the electroimmunoassay method. Transamidase activity of factor XIII was 26%. The level of factor XIII of her sister was low, similar to that of the patient. The concentration of cold-insoluble globulin in EDTA-plasma was 36.5 mg/dl.

Adult↗
PubMed1983

Treatment of DIC with antithrombin III concentrates.

We administered AT III concentrates to 21 patients with DIC who failed to respond initially to heparin therapy. About 60% of these 21 patients were effectively treated with AT III concentrates by enhancing the effect of heparin and alleviating the burden of excessive plasma transfusions on the cardiovascular system.

Adult↗
PubMed1983

"Fibrinogen Tokyo II". An abnormal fibrinogen with an impaired polymerization site on the aligned DD domain of fibrin molecules.

A hereditary dysfibrinogenemia associated with defective aggregation of fibrin monomers was found in a 39-yr-old female and in the members of her immediate family, who had all been asymptomatic. The abnormality was probably due to an impaired polymerization site exposed in the DD domain of two adjacent fibrin molecules, because plasmic fragment DD derived from the propositus' cross-linked fibrin bound far less tightly to insolubilized normal fragment E than that from the normal one. Its complementary polymerization site in the E domain of fibrin, which was exposed by thrombin cleavage, and the polymerization site in the D domain of fibrinogen, which was available without activation by thrombin, were both found to be normal. More anodal migration of the abnormal fragment DD than the normal one, as shown by immunoelectrophoresis, seemed to support our concept that the mutation most likely resides in the D domain of the abnormal fibrinogen molecule at or near a region closely related to the polymerization site that is exposed when two fibrin molecules are linearly aligned. The work of others on the polymerization of normal fibrin with different techniques yielded results consistent with our conclusions. We tentatively designate this type of abnormal fibrinogen "fibrinogen Tokyo II," but its possible identity with other abnormalities of fibrinogen reported heretofore is not excluded.

Adult↗
PubMed1983

Carbohydrate metabolism during pulsatile cardiopulmonary bypass under profound hypothermia.

Procedures related to profound hypothermia and circulatory arrest have produced a great improvement in the operative results of cardiac repair in neonates and infants. As we already obtained data on the effects of these procedures on cerebral metabolism, we focussed our attention on carbohydrate metabolism to determine whether or not cardiopulmonary bypass with pulsatile flow would improve the results of bypass method for core cooling and rewarming. In 12 mongrel dogs under conditions of hypothermia, plasma levels of glucose, insulin and glucagon as well as cortisol and noradrenaline were monitored in both pulsatile and non-pulsatile bypass groups. The hyperglycemia was significantly depressed and insulin levels increased in cases of pulsatile flow. Thus, even under conditions of hypothermia, pulsatile flow results in an improvement of the blood flow in the pancreas and there is a more extensive utilization of glucose and a greater protective effect on the function of the visceral organs during bypass.

Animals↗
PubMed1983

Sudden cardiac death at exertion.

In order to investigate sudden cardiac death at exertion (sports, and light work in daily life), 226 cases of sudden death during sports and 1,729 autopsy cases of sudden death were studied, and exercise tests were performed on 138 patients with various cardiac diseases. Among sports, marathon or jogging caused sudden death most frequently (52.7%). It occurred often immediately after goal (41.3%) or before goal (28.0%), but cases with a history of heart disease died at the beginning of the race. Severe heat, overwork and other factors may precipitate the sudden death. In autopsy cases of sudden cardiac death which occurred at exertion, coronary arteriosclerosis (C) was found in 67.0%, cardiac hypertrophy of unknown etiology (H) in 7.8% and valvular heart diseases (V) in 7.1%. Sudden death in patients with C or H were more frequent at exertion than at rest. Patients with C were older and died at light work. Those with H of a high degree also died at light work, but the younger ones with a mild H died at sports activity. At exercise test, in patients with arrhythmia or hypertrophic cardiomyopathy, abnormalities may occur after exercise. Sudden death at exertion may depend on the predisposing heart disease. Care should be taken especially at the end of the race.

Adolescent↗
PubMed1983

Studies on the antibody composition and neutralizing activity of tetanus antitoxin sera from various species of animals in relation to the antigenic substructure of the tetanus toxin molecule.

On the basis of the antigenic substructure of tetanus neurotoxin, the antitoxin compositions of horse, rabbit and human tetanus antitoxin sera, in terms of their contents of antibodies against four antigenic determinant groups (alpha, beta-1, beta-2 and the "topographic" determinant group gamma) so far known for the toxin were studied by quantitative precipitation reactions using purified toxin, complementary fragments alpha, beta and fragment beta-1 (a subfragment of fragment beta) of the toxin. The antitoxin antibody composition varied slightly depending on the antiserum preparation. In addition, different patterns of antitoxin antibody composition and toxin-neutralizing ability, characteristic of horse, rabbit and man were found: horse antitoxin sera contained all four kinds of antibodies and horse anti-gamma showed low toxin-neutralizing ability, while human antisera lacked anti-alpha and had anti-gamma with high neutralizing activity but contained anti-beta-1 with no detectable neutralizing activity. Rabbit sera showed an intermediate pattern between those of horse and human sera. In all antisera, antibodies against determinants on the isolated fragment beta account for approximately 80-50 percent of the total precipitable antibodies and anti-beta-2 antibody was invariably present. Immunodiffusion analyses showed that the antitoxin compositions of mouse and guinea pig antisera resembled those of human antisera. In mice, fragment beta was almost as efficient as whole toxin toxoid in eliciting a protective immune response on an equal weight basis, whereas fragments beta-1 and alpha were both relatively poor antigens.

Animals↗
PubMed1983

[Chemotherapy of small cell carcinoma of the lung in relation to lymph node metastasis and extending pattern of tumor].

Chemotherapeutic effects on the lymph node metastasis were studied in relation to the predominancy of T or N factor, extending patterns of tumor, histologic subtype, and cytologic characteristics, using cyclophosphamide, vincristine and 5-FU. N2, N3, N4 indicate ipsi-lateral, contra-lateral mediastinal, and supra-clavicular node, respectively. Chemotherapy was equally effective to the lymph node metastasis regardless a degree of progression, but survivals depended on the progression. Predominancy of both primary tumor (T) and involved lymph node (N) was analysed on chest X-rays. The Deffer response was observed in Type N than in Type T. Extending patterns of primary tumor were classified on X-ray films according to Suzuki's criteria: a longitudinal submucosal spreading along the bronchus (I): infiltration beyond the bronchial wall, extending to the depth, and fusing into a mass (III): and intermediate (II). In type I, the tumor was seen like an assembly of clubs extending along the bronchial tree, and Type III tumor showed a mass-like shadow with relatively high density and clear margin. Overall response rates were remarkably higher in Type I and lower in Type III. Type I was more frequently accompanied by large lymph node involvement than Type III. The highest response rate was obtained in Type I with N-predominancy and the lowest in Type III with T-predominancy. Responses to chemotherapy of lymph node metastasis depended upon these extending patterns. A half of Type III cases were polygonal cell type, which showed the lowest response rate in our institute. Regarding to cytologic classification by Horai, 67% response of good was found in Type I and 65% of response poor in Type III. These biological characteristics are considered to be a useful indicator for the selection of treatment modalities.

Carcinoma, Small Cell↗
PubMed1983

Long-term observations of the graft endothelium with different postoperative courses.

Using a specular microscope and a pachymeter, we monitored endothelial cell patterns and central corneal thickness in 16 cases of clear graft with different postoperative courses for over 2 years after successful penetrating keratoplasty. With the use of a computerized digitizer, the graft endothelium was analyzed to determine the mean cell size, standard deviation of cell sizes and the coefficient of variation in cell size. In all cases, a progressive increase in the mean and standard deviation of cell sizes was noted throughout the period of observation. The coefficient of variation, however, stayed fairly constant in each case. This indicates that the increase in the standard deviation of cell sizes is dependent on the increase in the mean cell size. Five cases with good postoperative course showed gradual alterations in cellular morphology. On the other hand, the endothelium of 5 cases with surgical intervention after keratoplasty changed its morphology greatly until the corneal thickness approximated normal, and then the alterations continued to take place gradually. In 6 cases, a remarkable and rapid increase in the mean and standard deviation of cell sizes occurred during the rejection process. These parameters continued to increase gradually after the graft became transparent as the result of intensive corticosteroid therapy. These results suggest that the graft endothelium is still in a transitional state during healing even 2 years after keratoplasty, and that the morphologic parameters determined during the early postoperative period reflect the actual cell damage owing to surgical procedures to some extent but not exactly.

Adolescent↗