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Biomedical subjects

M Mariani

Publications and source records attributed to M Mariani.

At least 253 records · Page 14Linked to original sources
PubMed1985

Production and characterization of monoclonal antibodies to anti-human chorionic somatomammotropin by immunization with two free synthetic peptides.

The peptides corresponding to the fragments 135-140 and 166-174 of human chorionic somatomammotropin (hCS) were synthesized, and used to raise monoclonal antibodies to the native hCS molecule. The synthetic peptides were injected into BALB/c mice in the free form, i.e. not conjugated to a carrier, and the spleens were fused with Sp2/01Ag8 myeloma line to produce monoclonal antibodies. The antibodies produced belonged to the IgM and IgG classes and, once purified by affinity chromatography on hCS-Sepharose, they were covalently coupled to macroporous polystyrene beads and characterized by competitive radioimmunoassay. Their affinity constants were determined by elaborating the radioimmunoassay data by nonlinear regression analysis and they were found to range from 10(5) to 10(6) M-1. The evaluation of the affinity constant of the antibodies produced is always important as a measure of the immunogenicity of an antigen, particularly when synthetic peptides are used as immunogens.

Animals↗
PubMed1985

Triosephosphate isomerase deficiency: 2 new cases.

2 new cases of triosephosphate isomerase (TPI) deficiency associated with severe haemolytic anaemia in 2 unrelated Italian families are described. Only 1 case was extensively investigated. TPI deficiency was detectable in erythrocytes, leucocytes, platelets and plasma. The mutant enzyme showed normal Km for GAP and increased Km for DHAP, with an higher than normal equilibrium constant, decreased thermostability, and abnormal electrophoretic pattern due to the lack of the fastest moving component. The immunological characterization revealed a lower than normal inactivation by specific antiserum, while the double immunodiffusion pattern and the precipitin curve were normal. Lymphocyte, granulocyte and platelet functions were impaired.

Blood Platelets↗
PubMed1985

Carotid body tumors. A report of two cases and review of the literature.

Two recently observed cases of carotid body tumor (chemodectoma) are presented. The treatment was subadventitial resection of the tumor with good long-term results. The clinic, diagnostic and therapeutic problems of such tumors are re-examined on the basis of the most recent informations obtained from a wide review of the literature.

Adult↗
PubMed1984

Antigenic structure of human chorionic somatomammotropin: four epitopes revealed by monoclonal antibodies.

The characterization of epitope specificity of a panel of 15 monoclonal antibodies against human chorionic somatomammotropin (hCS), previously prepared in our laboratory, allowed us to distinguish 4 antigenic determinants on the hCS molecule. Experiments were carried out with competition and sandwich assays. The results allowed us to divide our MAbs into 4 groups, recognizing 4 different epitopes, and to select 4 MAbs each distinguishing a different epitope in order to pursue further studies on the antigenic structure of hCS.

Antibodies, Monoclonal↗
PubMed1984

Characterization of monoclonal antibodies against human chorionic somatomammotropin: competitive screening and determination of the affinity constants.

A simple screening method has been developed for the characterization of monoclonal antibodies (MAbs) against human chorionic somatomammotropin (hCS) produced in our laboratory. The method is easily adaptable to MAbs against other soluble antigens. Our proposed method allows evaluation of the degree of binding of the antibodies to the antigen, and of cross-reaction with human growth hormone and ovine prolactin. The positive clones have been further characterized by solid-phase radioimmunoassay in order to determine their affinity constants, which ranged from 10(7) to 10(9)/M as determined by non-linear regression analysis.

Animals↗
PubMed1984

Identification and synthesis of an antigenic determinant common to human chorionic somatomammotropin and human growth hormone.

Human chorionic somatomammotropin (hCS) and human growth hormone (hGH), despite their different biological activities, show a remarkable degree of homology in their primary structure, which explains their immunological cross-reactivity. On the basis of the hydrophilicity profiles, we predicted that the sequence 165-174 would correspond to an antigenic determinant common to both hormones. The sequence 165-174 was synthesized in the solid phase and the ability to bind antibodies to hCS was tested by a radioimmunoassay at each step of the synthesis, without detaching the peptide from the resin. We found that the immunological sequence able to bind antibodies to hCS is that corresponding to 167-174. In similar experiments, we showed that the sequence 166-174 is able to bind antibodies to hGH. In the plaque-forming cell test using the synthetic fragment 166-174 bound to sheep red blood cells, we also observed that the ratio between the number of cells forming antibodies to hCS and to the 166-174 peptide was always between 0.45 and 0.73, thus suggesting that the 166-174 peptide represents a major determinant of hCS.

Amino Acid Sequence↗
PubMed1984

A new test for the laboratory diagnosis of spherocytosis.

A new test for the laboratory diagnosis of spherocytosis, conventionally called 'Pink test', is presented. This test, semi-quantitatively or quantitatively, determines the hemolysis of small blood samples in a solution containing glycerol (135 mmol/l), NaCl (25 mmol/l), NaN3 (1.5 mmol/l), buffered to pH 6.66 with Bis-Tris (70 mmol/l) and HCl. 'Pink test', as well as 'acidified' glycerol lysis test, were positive in 100% of 42 patients suffering from hereditary spherocytosis, and optimally discriminated them from healthy subjects, showing a diagnostic sensitivity greater than 'standard' glycerol lysis test and osmotic fragility in hypotonic saline solutions of fresh or incubated blood. 'Pink test' was also positive in some cases of renal failure, immunohemolytic anemia, chronic hemoproliferative disorders, normal pregnant women, and negative in other microcytic anemias (beta-thalassemia, iron deficiency anemia). The results do not critically depend on pH of the solution (differently from those obtained with 'acidified' glycerol lysis test), and for this reason they show a good reproducibility.

Buffers↗
PubMed1984

Primary retroperitoneal tumors: report of 21 cases.

A personal series of 21 cases of primary retroperitoneal tumors (PRT) are reported and the clinical features, diagnostic assessment, surgical treatment and results are examined. The problem of the complementary radio-chemotherapy, which is mandatory following surgical resection, is also dealt with. It is underlined that long-term results are improved only by early diagnosis and therefore the use of the most recent technical procedures, such as echotomography, computed tomography, and angiography, is stressed.

Adolescent↗
PubMed1983

Simple immunization protocol for high frequency production of soluble antigen-specific hybridomas.

We report the immunization protocol used to produce high frequency of specific hybridomas secreting defined antibodies against soluble proteins. The two immunization schemes used consist in 1300 and 115 micrograms of protein distributed over a period of two weeks. The specific efficiency (SE) of positive clones recovered in eleven different fusion experiments ranged between 0.52 and 0.78. These values are referred to hybridomas secreting monoclonal antibodies (MAbs) against soluble proteins such as human chorionic somatomammotropin (hCS), human growth hormone (hGH), and human prostatic acid phosphatase (hPAP). Similar high SE were also recovered by immunizing mice with nonsoluble antigens such as intermediate filaments of cytoskeleton (IF).

Acid Phosphatase↗