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Biomedical subjects

M Marcacci

Publications and source records attributed to M Marcacci.

At least 37 records · Page 2Linked to original sources

Myoelectric activation pattern during gait in total knee replacement: relationship with kinematics, kinetics, and clinical outcome.

Gait usually presents an excellent improvement after total knee replacement. Nevertheless, some abnormalities persist even after a long period of time. The abnormal knee patterns have been attributed to several possible causes, such as implant geometry and surgical technique, posterior cruciate ligament sparing/sacrificing, preoperative "stiff-knee" pattern due to pain and altered biomechanics, weakness of the extensor muscles, preoperative arthritic pattern, proprioceptive deficiency, and multijoint degenerative involvement. Cocontraction of the knee flexors and extensors is a common strategy adopted to reduce strain and shear forces at the joint, but it increases compressive forces and joint loading. Even in patients with an excellent functional score, the duration of the implant may be compromised by an altered neuromuscular control of the knee. In this paper, we report a single case study carried out over two years on a patient that underwent total knee replacement. The aim of this work is to show that quantitative gait analysis is essential to augment the understanding of the mechanisms underlying gait, thus enabling clinicians to adapt the rehabilitation program to the specific patient. Although the limits of single case reports are obvious, we believe that this evaluation methodology could be beneficial for assessing the effectiveness of rehabilitation programs aimed at achieving an active control of the knee during gait through a correct muscular activation pattern.

Biomechanical Phenomena↗

Use of autologous grafts for reconstruction of osteochondral defects of the knee.

This study reports on 13 patients (mean age: 31 years) with a femoral condyle defect >1.5 cm2 who underwent treatment with an osteochondral graft of the same size obtained from the superior aspect of the lateral condyle, preserving the patellar groove. Mean follow-up was 61.5 months (range: 13-141 months). Twelve results were rated clinically as satisfactory with patients able to resume their normal pre-injury level of activity, and 1 case was rated as poor. No patient reported any patellar problems. Radiographic and computed tomographic evaluation demonstrated good integration of the graft in the host bone. The results of this technique at relatively long-term follow-up are encouraging, with a high percentage of subjective satisfaction. This technique appears to be reliable and provides a valid solution for treatment of wide cartilage defects when other techniques are too complex or inadequate.

Adolescent↗

Fiber-based anterior cruciate ligament model for biomechanical simulations.

Although accurate predictions of the behavior and function of the anterior cruciate ligament would aid in diagnosis and operative treatment, no agreement exists on the best way to model the ligament with a reliable description of its mechanical and geometric features. We propose a new model of the anterior cruciate ligament based on multiple viscoelastic curvilinear fibers. This model was used for quasi-static simulations of the passive motion of eight porcine knees, after registration of the passive trajectories and digitization of the surface of the ligament in flexion. Simulations of anterior cruciate ligament deformations during passive motion predicted a fiber strain of less than 20%, low insertion forces, and isometric anterior fibers. The model explains actions of the ligament fibers, as reported in the literature, better than classic models based on linear fibers and consistent with the mechanical properties of the anterior cruciate ligament.

Animals↗

Arthroscopic intra- and extra-articular anterior cruciate ligament reconstruction with gracilis and semitendinosus tendons.

Numerous surgical procedures have been developed and used for anterior cruciate ligament (ACL) reconstruction. Patellar tendon is probably the most common graft used, but gracilis and semitendinous tendons present some interesting advantages: small incision, large graft when doubled, characteristics close to ACL, rapid harvest. We describe a combined intra- and extra-articular arthroscopic ACL reconstruction using hamstring tendons which includes some original steps. The tendons are harvested, leaving the distal insertion intact, and sutured together. After drilling of the tibial tunnel, an over-the-top arrangement is formed, creating a groove in the posterolateral aspect of the femur. The tendons are then fixed with double staples in the groove, and their remaining part is fixed distally to Gerdy's tubercle passing under the fascia, but over the lateral collateral ligament (LCL). This technique ensures sufficient strength in the graft and permits correction of any associated instability, because of the presence of the extra-articular portion of the tendons. Furthermore, the over-the-top arrangement reduces trauma and possible pitfalls related to tunnel construction and permits isometry of the extra-articular portion to be established. Forty patients involved in sports activity were prospectively selected and evaluated at a minimum 2 years' follow-up. IKDC score and Lysholm score were used for clinical evaluation, and the KT-2000 was used for instrumental laxity measurements. Resumption of sport and time to that point were recorded as well as Tegner activity score. We had 92.5% normal and fairly normal knees according to IKDC score and only 7.5% abnormal knees. Mean Lysholm score was 95. Mean Tegner score was 7.2. KT-2000 showed a mean injured/uninjured difference of 2.1 mm. In all, 90% of patients resumed sports at the same level, 67.5% in 3-4 months and 27.5% in 4-6 months. The highly satisfactory results of this series with no major complications confirm the reliability of this technique and the possibility of guaranteeing functional behaviour in the knee.

Adolescent↗

Total knee arthroplasty kinematics. Computer simulation and intraoperative evaluation.

The goal of this work was to develop computer simulations for intraoperative testing of the passive kinematics of knee prostheses. These are based on an anatomic model of the reconstructed joint, represented in the sagittal plane. A femoral component and a tibial component are linked by 3 springs that model the relevant ligaments, with the posterior cruciate providing the primary constraint. The components' behavior at each flexion angle is obtained by minimizing the total strain energy stored in the ligaments. Simulations were performed in vivo on 10 Interax implants (Howmedica International, Stain, UK) and showed good agreement with intraoperative observations, allowing monitoring of new parameters such as contact point motion, ligament strains, and the energetic state of the knee. This work contributes to the comprehension of individual knee kinematics after total knee arthroplasty, to improve long-term results and standardize the evaluation of the results of joint restoration.

Arthroplasty, Replacement, Knee↗

pO2 measurement in an experimental model of patellar tendon autograft pro-anterior cruciate ligament.

Thirty-four sheep were submitted to surgery substituting the native ACL with the central third of the patellar tendon, ten enter this study. The purpose was to find a possible relationship between tissue pO2 and healing processes considering also the biomechanical and histomorphological aspects of the grafts. Four of them were sacrificed under general anaesthesia after 6 months, and six after 1 year in order to perform tissue pO2 measurement and an analysis of microvessel density on specimens of the normal ACL and the graft. Our data showed higher pO2 values of the autografts after 6 months. After 1 year the data was comparable to those of native ACL. This was confirmed by a microvessel count of the histological specimens and the data was in relationship to biomechanical and histomorphological analysis. Tissue pO2 can be observed and recorded in "in vivo" ACL, and patellar tendon used as graft, with no injury to their integrity. The monitoring system might be considered as an experimental tool for indirect controls of the anterior cruciate substitutes.

Animals↗

Cryo/Cuff use after arthroscopic surgery: effect on knee joint temperature.

Any modality that alters joint homeostasis could have a potential negative effect on cartilage. During arthroscopic knee surgery, the temperature in the knee joint decreases significantly. The use of cryotherapy after this surgical procedure could maintain or increase these temperature variations. This prospective, randomized study evaluated the change in intra-articular knee temperature with the use of the Cryo/Cuff (Aircast Inc, Summit, New Jersey) in 30 patients after arthroscopic knee surgery. In 15 patients, a Cryo/Cuff with an automatic pump was applied immediately after surgery (group 1), and in the remaining 15 patients, only a wound dressing was applied (group 2). The mean intra-articular temperature at the beginning of the procedure was 34.8+/-1 degrees C for both groups. At the end of surgery, the temperature was 26.8+/-2.2 degrees C for group 1 and 27.5+/-2.2 degrees C for group 2. One hour after the end of the procedure, the mean temperature was 34+/-1.6 degrees for group 1 and 34.8+/-1.7 degrees C for group 2. These differences were not statistically significant. These results demonstrated that the use of the Cryo/Cuff does not alter temperature recovery in the knee, thereby excluding potential damage to the articular cartilage.

Adult↗

Minimally invasive 3D data registration in computer and robot assisted total knee arthroplasty.

Computer and robot assisted surgery is concerned with the improvements achievable by using computer methods and robotic devices to plan and execute surgical interventions. The registration of different coordinate frames, often achieved through the matching of 3D data sets, represents a crucial step connecting planning and execution. Orthopaedic surgery already features a number of functioning applications which include registration routines relying on presurgically implanted fiducial markers. Replacing such invasive routine with non-fiducial registration procedures is regarded as a necessary step towards a minimisation of surgical invasiveness. A minimally invasive registration technique based on the iterative closest point algorithm is presented and conceived for a specific computer and robot assisted orthopaedic reconstructive intervention, namely total knee arthroplasty. The whole surgical protocol is examined in detail and the experimental results, relative to tests performed on synthetic and animal specimens, are thoroughly reported and discussed. The authors indicate that the proposed registration approach is well-suited for the relevant application and appropriate for in vivo testing.

Algorithms↗

Histological and ultrastructural evaluation of Leeds-Keio ligament six years after implant. A case report.

We examined by light and electron microscopy study a Leeds-Keio ligament removed from a patient 6 years and 4 months after implant following rupture. The new ligament presented an outer capsule made up of bundles of collagen fibres running mainly perpendicular to the long axis of the ligament. Septa were seen emerging from the capsule and composed of bundles of collagen fibres surrounding the bundles of Dacron fibres. Each thread of Dacron was surrounded by a layer of connective tissue containing periodic acid-Schiff (PAS)-positive cells. The bundles of collagen fibres making up the outer capsule, the septa and the layer of connective tissue surrounding the Dacron threads were positive for anti-type I collagen antibody. The rehabitated Leeds-Keio ligament presented a specific organization at the septa zone, showing a layer of collagen fibrils alternating with a layer of cells. Our remodelling findings suggest a shoelace effect of the artificial ligament. On the other hand, the presence of type I collagen could be responsible for the good functional behaviour of this composite system. In conclusion, the factors that play an important role in determining this remodelling process and its mechanical function are unknown.

Adult↗

Total knee arthroplasty without patellar resurfacing in active and overweight patients.

Overweight patients are often considered poor candidates for total knee arthroplasty (TKA). A retrospective study of this was done on 47 osteoarthritic knees treated by TKA without patella resurfacing between March 1991 and June 1993. The Hospital for Special Surgery (HSS) rating system was used for clinical evaluation, and radiographs to study the degree of osteoarthritis and radiolucency. Correlations between overweight, range of motion (ROM) and stage of patellar damage and other measured variables (HSS score, patellar pain and radiolucency) were studied. Overweight was not correlated with HSS score, radiolucency or patellar pain. ROM was significantly correlated with patellar pain and HSS score, with better results in patients with ROM between 90 degrees and 110 degrees. Therefore, we believe that TKA in osteoarthritic knees can lead to successful results, even in active or overweight patients.

Aged↗

Arthroscopic reconstruction of the anterior cruciate ligament with Leeds-Keio ligament in non-professional athletes. Results after a minimum 5 years' follow-up.

We report our experience using the Leeds-Keio artificial ligament for anterior cruciate ligament (ACL) reconstruction. The study relates the results of the first 40 patients subjected to arthroscopic reconstruction of the ACL with a Leeds-Keio ligament, with a mean follow-up of 73 months. No associated peripheral procedures were carried out on any patient. The average age of the patients at the time of the operation was 31 years (range 26-35 years). The rehabilitation protocol followed by all patients aimed at resumption of sport 4 months after the operation. Clinical assessment included IKDC and the Lysholm scoring scale. The KT-2000 system was used for instrumented evaluation of joint laxity. All patients underwent a radiographic check-up. Clinically there were 55% excellent or good results when using the IKDC scale, while with the Lysholm score, satisfactory results were obtained in 80%. Complete post-traumatic rupture of the ligament was observed in three patients. No patient suffered an episode of either hydrarthrosis or reactive synovitis, which indicates good tolerance to the ligament. The radiographic evaluation of the operated knees showed a close correlation between the appearance of degenerative phenomena and performance of arthroscopic meniscectomy. The results achieved with the Leeds-Keio artificial ligament 5 years after application, although not completely satisfactory and inferior to those obtained with autologous biological ligaments, should be considered an encouragement to promote new efforts in this interesting research field.

Adolescent↗

Arthroscopic management of recurrent anterior dislocation of the shoulder: analysis of technical modifications on the Caspari procedure.

Arthroscopic treatment was performed on 71 patients with recurrent shoulder dislocations; all of the patients had monoplanar anterior instability attributable to arthroscopically diagnosed Bankart lesion. Of the 71 operations, 29 were performed using the original Caspari technique (follow-up, 59 months), and 42 were performed after modifications made in the original technique (follow-up, 38 months), notably improved preparation of the capsular reinsertion zone and increase in the number of monofilament points and their anchorage directly to the bone, on the spine of the scapula. We compared the results obtained in these two differently treated groups, taking into account several factors in the patient's history and clinical condition. These included the number of dislocation episodes before the operation, as well as clinical findings regarding stability, movement, function and pain (Rowe scale score), contralateral shoulder laxity, level of preoperative versus postoperative athletic activity, and postoperative recurrence rate. In the Caspari-treated group, we obtained 66% satisfactory results compared with the 90% obtained in the second group. The recurrence rate was 27% in the first group compared with 4.8% in the second group. These data were statistically significant. No correlation was found between preoperative number of dislocations and recurrence rate, nor for contralateral shoulder laxity. No significant difference was found regarding resumption of sport activity in the two groups. Our data indicate that, with accuracy in patient selection and effective surgical technique, the recurrence rate can be reduced, and results similar to those of the arthrotomic technique may be obtained.

Adolescent↗

Use of a biocompatible fiducial marker in evaluating the accuracy of computed tomography image registration.

RATIONALE AND OBJECTIVES: Accurate registration of computed tomography (CT) images to the patient is crucial for computer-assisted surgery. Markers used in roentgen stereo-grammetric analysis (RSA) can be located in a CT scan using a novel approach and also can be located physically. Roentgen stereo-grammetric analysis data act as "ground truth" for the three-dimensional marker locations. METHODS: Two foam-bone phantoms were marked. The markers were scanned seven times with RSA, three times with axial CT, and contacted four times with a coordinate measuring arm. Root-mean-square (RMS) errors were derived for the registrations. RESULTS: Computed tomography and RSA data register to 0.15 mm RMS error. Computed tomography and arm data register to 0.25 mm. The markers are biocompatible, and the coordinate measuring arm is usable in an operating room. CONCLUSIONS: Typical in vitro registration errors are approximately 2 mm. The authors have developed a marker that provides superior registration, and a procedure that can be used for in vivo studies.

Biocompatible Materials↗

Results in the treatment of recurrent dislocation of the patella after 30 years' follow-up.

The authors compare the clinical and radiographic outcome in patients with comparable bilateral recurrent patellar dislocation treated surgically on only one side, to clarify the appropriateness of the surgical indication. Sixteen patients were evaluated at an average follow-up of 30 years (20-45); all had been treated by the Roux technique. The results on both the operated and the unoperated knee were evaluated; the Crosby and Insall schedule was used for the clinical evaluation. Anteroposterior, lateral, and Merchant's view X-rays were examined for osteoarthritis and to measure the height of the patella. The congruence angle and the distance between anterior tibial tuberosity and trochlear groove (ATT-TG) were measured by computed tomography. The results in the operated knees were: 3 excellent, 9 good, 1 fair, and 3 worse; results in the nonoperated knees were 6 excellent, 8 good, 1 fair, and 1 worse. In the operated knees arthritis was grossly marked in 8, marked in 3, moderate in 1, and light in 4; in the nonoperated ones it was grossly marked in 8, moderate in 3, and light in 5. The congruence angle was normal in 10, medially displaced in 3, and laterally displaced in 3 cases on the operated side; on the nonoperated side it was normal in 7 cases and lateralized in the remaining 9. The ATT-TG in the operated knees was negative in 9 cases, normal in 1, and positive in 6; on the non-operated side it was positive or normal. In 7 operated cases a low patella was documented. The Roux technique yields positive results in the correction of recurrent dislocation. No clinical or radiographic differences were found between surgically and conservatively treated knees. The clinical results are generally not comparable with the radiographic features or with severity of degenerative modifications presented at long-term follow-up. The absence of a difference is due basically to the complete lack of adaptation of the surgical procedure to the variable pathogenesis of this disorder.

Adolescent↗

Comparison between laser meniscectomy with excimer and Ho:YAG lasers.

The authors report their experiences using the excimer laser and holmium:YAG laser in arthroscopic meniscal surgery. Histologic and ultrastructural aspects caused by laser irradiation on meniscal tissues are evaluated. Two groups of 10 patients, each suffering from a bucket-handle lesion of the internal meniscus, were used for this study. The histologic results of the study confirm the clinical reliability of the laser even in the presence of different ultrastructural frames.

Arthroscopes↗

Functional evaluation of patients fitted with total knee prostheses.

Degenerative or inflammatory diseases of the knee joint account for a large proportion of diseases affecting the elderly. Treatment aims to eliminate pain, correct the deformity and maintain complete joint mobility. In cases of total joint degeneration, fitting of a joint prosthesis is the technique currently favoured since elimination of pain and correction of the deformity, accompanied by recovery of joint mobility (the fundamental requirements for normal everyday life) can only be obtained if the knee joint is completely replaced. With the discovery of new materials and increasingly sophisticated knowledge about articular mechanics, the knee prosthesis, although more recent than the hip prosthesis, has become ever more widespread to the point that it now represents an irreplaceable aid in the treatment of this common disease. The clinical results obtained are already stable and particularly encouraging and prosthesis design is sophisticated and accurate. There is, however, still the suspicion that current prostheses can be considered "rudimentary" in comparison with the physiological and biomechanical behaviour of the human joint. We therefore wanted to begin an evaluation using a gait analysis system to assess patients fitted with knee prostheses. The first impressions gained in this study are the subject of this article.

Activities of Daily Living↗

The human anterior cruciate ligament: histological and ultrastructural observations.

In transverse and longitudinal paraffin-embedded sections, the human anterior cruciate ligament (ACL) is made up of wavy bundles of collagen fibres arrayed in various directions, the majority around the axis of the ligament with a few running parallel to it. The fascicles making up the larger bundles are also characterised by this undulating appearance. In thin sections 2 types of collagen fibrils are observed: small (with a single diameter peak at 45 nm) and large (3 peaks at 35, 50 and 75 nm respectively), organised into distinct areas made up of either large or small bundles of fibrils. The numerous fibroblasts that are present appear elongated in the direction of the bundles with branches and short cytoplasmic processes. The elastic system is made up of both elastic and oxytalan fibres. The varied orientation of the bundles in the ACL, the complex ultrastructural organisation and the abundant elastic system make it very different from other ligaments and tendons, providing a structure able to withstand the multiaxial stresses and varying tensile strains imposed upon it.

Anterior Cruciate Ligament↗