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Biomedical subjects

M Mann

Publications and source records attributed to M Mann.

297 records · Page 17Linked to original sources
PubMedDate not supplied

Glucocorticoidlike activity of megestrol. A summary of Food and Drug Administration experience and a review of the literature.

Sporadic single case reports linking glucocorticoidlike activity to megestrol acetate have been reported in the literature. These findings have important implications for patient care. Adverse drug experience reports to the US Food and Drug Administration from 1984 through 1996 and a MEDLINE search of the literature from 1984 through 1996 provided the case reports. Five cases of Cushing syndrome, 12 cases of new-onset diabetes, and 16 cases of adrenal insufficiency were identified in association with megestrol therapy. Twelve cases in which preexisting diabetes was exacerbated and 17 cases of possible adrenal insufficiency were identified. Therapy with megestrol can result in clinical manifestations of glucocorticoidlike activity, including Cushing syndrome, diabetes, and adrenal insufficiency. Clinicians need to be aware of this association as these complications can be life-threatening if not recognized.

Adrenal Insufficiency↗
PubMedDate not supplied

Identification of transformation sensitive proteins recorded in human two-dimensional gel protein databases by mass spectrometric peptide mapping alone and in combination with microsequencing.

A comprehensive human keratinocyte two-dimensional (2-D) gel protein database has been established to study the expression levels and properties of the thousands of proteins that orchestrate various keratinocyte functions both in health and disease, cancer included. A major task in establishing such a database is to identify known proteins in the 2-D gel patterns as well as to reveal hitherto unknown proteins. To date, protein identification has been performed by one or a combination of the following methods: (i) comigration with known proteins, (ii) Western blotting using specific antibodies, (iii) microsequencing and (iv) vaccinia virus expression of full length cDNAs. Recently, the systematic identification of proteins has gained a new dimension with the advent of computer programs for searching peptide molecular mass databases with experimentally obtained peptide mass maps. Here we investigate this approach to identify proteins that are highly up- or down-regulated in simian virus SV40 transformed human keratinocytes (K14). Peptide mass maps of several proteins, including keratins 7, 8, 18 and 19 were obtained either by plasma desorption mass spectrometry (PDMS) analysis of high performance liquid chromatography (HPLC) purified peptides or by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of total digests. The results demonstrated that peptide mass maps can be used for a rapid and sensitive protein identification allowing fast screening of proteins recorded in 2-D gel databases. The mass spectrometric approach when combined with microsequencing strengthened identification, and added the possibility of full characterization of post-translational modifications and sequence variations.

Amino Acid Sequence↗
PubMedDate not supplied

Safety of autologous blood donation prior to elective surgery for a variety of potentially "high-risk" patients.

We studied 342 potentially "high-risk" patients, including patients with severe heart disease, elderly patients, children, and pregnant women, to determine the incidence of complications related to phlebotomy. Our patients had an adverse reaction rate associated with blood donation of 4 percent, which is no greater than would be expected among normal volunteer blood donors. No patient experienced a dangerous complication associated with phlebotomy. We conclude that many patients currently denied the opportunity to donate blood preoperatively are actually suitable candidates for autologous blood transfusion programs.

Adult↗
PubMedDate not supplied

[Fatigue fractures of the lower patellar pole in adolescents with cerebral movement disorders].

Changes at the apex of the patella, seen on X-rays of 4 children with cerebral motility disturbances, raise the question as to whether the changes seen in patella tip syndrome are an event typical for the underlying condition. The present authors are of the opinion that these changes, which resemble patella partita, Sinding-Larsen-Johansson disease and osteopathia patellae juvenilis (C. Mau), do not represent a separate syndrome. The authors believe that all these changes are merely variants of one and the same event, namely a disturbance of the balance between loading and loadbearing capacity of the insertion of the Lig. patellae, a balance which is evidently especially unstable at this age.

Adolescent↗
PubMedDate not supplied

[Rotator sheath ruptures--surgical treatment and results].

Between 1972 and 1982 45 patients with tears of the rotator cuff have been operated in our clinic. 41 of these patients could be controlled at an average of 3.5 years after the operation. According to the applied repair technique the observed patients were divided into three groups. The results were analysed in regard to this classification. A very good and good active movement was achieved in 2/3 of the cases.

Adult↗
PubMedDate not supplied

Use of mass spectrometric methods for protein identification in receptor research.

In recent years, mass spectrometry has become the method of choice for identifying small amounts of gel separated proteins. Using high mass accuracy peptide mass mapping followed if necessary by nanoelectrospray sequencing, most mammalian proteins can now be identified quickly and sensitively either in amino acid or in EST sequence databases. These methods are illustrated here using an ongoing project in the author's laboratory, a mass spectrometric screen for new mouse brain receptors and their interaction partners.

Animals↗