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Biomedical subjects

M Manabe

Publications and source records attributed to M Manabe.

At least 73 records · Page 4Linked to original sources
PubMed1995

Detection of deiminated proteins in rat skin: probing with a monospecific antibody after modification of citrulline residues.

We performed a systematic study on deiminated proteins present in rat epidermis. Proteins extracted from various epidermal samples were resolved by either one- or two-dimensional gel electrophoresis and Western blotted to nitrocellulose membranes. Deiminated proteins were detected by modification of citrulline residues followed by probing with an anti-modified citrulline monospecific antibody. The cornified layer of adult plantar skin gave multiple series of isoelectric variants, most of which were found to be differentially deiminated type II keratins (60 kDa, and 67 kDa or above). The whole epidermis of 5-day-old rat back skin showed isoelectric variants of 60-kDa keratin as major deiminated components, and deiminated 55-kDa keratin and deiminated filaggrin as minor spots. In addition, we found highly deiminated proteins (200-220 kDa) thought to be derived from trichohyalin. The immunoreactivity of deiminated proteins was mainly localized in the granular and cornified layers of epidermis. Co-localization of deiminated filaggrin and keratins in the granular layer suggests the possible role of protein deimination during the terminal stage of epidermal differentiation.

Animals↗
PubMed1995

Cutaneous ultrastructural features of the flaky skin (fsn) mouse mutation.

An autosomal recessive genetic disease with clinical and histopathological skin features resembling human psoriasis vulgaris occurs naturally in flaky skin mice (fsn/fsn). Affected mice are normal at birth, except for a hypochromic anemia. Subsequently, they develop hyperkeratotic plaques and acanthosis with elongation of rete ridges. Scanning electron microscopic examination revealed a greatly thickened epidermis, a sparsity of hairs and scale accumulations on the epidermal surface. Hair shafts had conspicuous pits, striations, and exophytic protrusions. Nails were bent at a 90 degrees angle with surface irregularities and accumulations of scale at the nail base. Transmission electron microscopic examination showed increased epidermal thickness, mitochondrial aberrations, and intraepidermal invasion by neutrophils. Keratohyalin abnormalities were detected using immunocytochemical staining for profilaggrin. At the dermal-epidermal junction, numerous macrophages and mast cells were seen in close proximity to focal dissolutions of the basement membrane. A high density of collagen fibers and cellular infiltrates were evident in the papillary dermis. This constellation of ultrastructural aberrations is typically found in psoriasis vulgaris and supports the theory that the flaky skin mouse mutation is a naturally occurring analog to one variety of human psoriasis vulgaris.

Anemia, Hypochromic↗
PubMed1995

Local anesthetics enhance nitric oxide production by human peripheral neutrophils.

Various neutrophil functions are suppressed by local anesthetics. We studied the effect of local anesthetics on nitric oxide (NO) generation in human peripheral neutrophils. Lidocaine and other local anesthetics stimulated NO generation in resting neutrophils. Canavanine, an NO synthase inhibitor, inhibited NO generation. Lidocaine and the other local anesthetics enhanced formylmethionyl-leucyl-phenylalanine (FMLP) or phorbol myristate acetate (PMA)-induced NO generation. These findings suggest that NO mediates various pharmacological effects of the local anesthetics on the host defense mechanism and the control of blood pressure.

Anesthetics, Local↗
PubMed1995

[Cephalad movement of the carina due to CD (Cotrel-Dubousset) instrumentation for idiopathic scoliosis].

Recently we experienced a case of tracheal tube obstruction due to the upward displacement of carina during CD (Cotrel-Dubousset) instrumentation for idiopathic scoliosis. Therefore, we hypothesized that CD instrumentation may affect the position of the carina as a result of correction of chest deformity, and studied the displacement of carina before and after CD instrumentation on chest X-ray in 12 patients with scoliosis. The length between the bottom of the 6th cervical vertebral body and the carina was measured by using chest X-ray before and after the correction. In all patients studied, cephalad movement of the carina was observed, and it was more than 1.00 cm in 5 patients. The maximal movement of the carina was 1.59 cm. The tracheal length after the correction was significantly shorter than before (P < 0.01). These results suggest that the carina may be displaced upwards due to the correction by the CD instrumentation, and that such induced displacement may affect the tracheal tube position and obstruct the tube during the operation.

Adolescent↗
PubMed1994

Distribution of immunoreactive malondialdehyde-modified low-density lipoprotein in human serum.

We developed a sensitive enzyme-linked immunosorbent assay (ELISA) for detection of malondialdehyde-modified low-density lipoprotein (MDA-LDL) in human serum. A monoclonal antibody against MDA-LDL (ML25) used in our method recognized not only MDA-LDL but also other MDA-modified proteins. However, MDA-LDL was able to be detected specifically by using a combination of ML25 and an antibody specific for apolipoprotein B (apo B) (AB16), which was conjugated with beta-galactosidase. Using this method, measurable amounts of MDA-LDL were detected in the sera of 40 healthy individuals. MDA-LDL was observed to be mainly distributed in the human LDL fraction separated by density gradient ultracentrifugation, while in each lipoprotein subfraction the largest amount of MDA-LDL per protein was found at a subfraction between LDL and HDL. The particle size of LDL in this fraction was smaller than that of LDL in the main LDL fraction, as assessed by electrophoresis. In addition, LDL oxidized by Cu2+ was also detectable with this method. We conclude that our method is sensitive and specific for MDA-LDL and might be useful for investigating MDA-LDL in the human circulation.

Antibodies, Monoclonal↗
PubMed1994

Effects of alcohol on lipoprotein lipase, hepatic lipase, cholesteryl ester transfer protein, and lecithin:cholesterol acyltransferase in high-density lipoprotein cholesterol elevation.

The mechanism whereby alcohol increases high-density lipoprotein cholesterol (HDL-C) levels is unclear. Lipoprotein lipase (LPL), hepatic lipase (HL), cholesteryl ester transfer protein (CETP) and lecithin:cholesterol acyltransferase (LCAT) act on lipoprotein metabolism. The purpose of the present study is to determine which one or what combination of these factors is responsible for the rise in HDL-C levels following alcohol ingestion. After 3 weeks of abstinence, 12 men consumed 0.5 g/kg bw of alcohol per day for 4 weeks; 13 abstaining men served as controls. Mean plasma total cholesterol (TC) levels were unchanged in either group throughout the study. Among the alcohol consumers, plasma triglycerides (TG), HDL-C, apolipoprotein (apo) A-I and A-II levels increased significantly after 3 weeks of alcohol loading but were unchanged in the control group. High-density lipoprotein3 cholesterol (HDL3-C) levels increased significantly in the alcohol consumers after 4 weeks of alcohol loading whereas high-density lipoprotein2 cholesterol (HDL2-C) levels were unaffected. In the controls, neither HDL2-C nor HDL3-C changed significantly. Post-heparin plasma (PHP) LPL activity and mass increased significantly (P < 0.01) after the alcohol ingestion (controls remained unchanged) without changing LPL specific activity. HL, CETP and LCAT activities were unaffected in both groups. We conclude that of the factors considered, LPL contributed the most to the alcohol-induced rise in HDL-C.

Adult↗
PubMed1994

Existence of trichohyalin-keratohyalin hybrid granules: co-localization of two major intermediate filament-associated proteins in non-follicular epithelia.

Trichohyalin is a protein of relatively high molecular weight (approximately 200 kDa), associated with intermediate filaments, that was for many years thought to be expressed only in the inner root sheath and medulla of the hair follicle. We show here, however, that this protein is expressed in association with (pro)filaggrin in the granular layer of many non-follicular, keratinizing, stratified epithelia which also express keratins K6/K16, including those of the filiform papillae of dorsal tongue epithelia. In this epithelium, which elaborates morphologically heterogeneous keratohyalin granules in its upper cell layers, trichohyalin forms hybrid granules with filaggrin, the major intermediate filament associated protein found in keratohyalin granules, which is normally expressed in advanced epidermal differentiation. These two intermediate filament-associated proteins remain physically segregated in the hybrid granules, but they share the same fate, as they both become dispersed in transitional cells, and are undetectable in cornified cells. Trichohyalin was also detected in nail matrix epithelia, the epithelium of Hassal's corpuscles of the thymus, and newborn foreskin epidermis. It is essentially absent from normal trunk and scalp epidermis, but is expressed in a few scattered cells of the granular layer that are also filaggrin-positive. In addition, trichohyalin is expressed in the epidermis in a number of hyperplastic skin diseases. These findings demonstrate that trichohyalin is not peculiar to a small number of hair follicle cells, but is expressed in a number of normal and pathological epithelia where it is uniquely associated with filaggrin. In addition, since all these trichohyalin-expressing keratinocytes also synthesize keratins K6 and K16 (the markers for an "alternative" pathway of keratinocyte differentiation), this raises the possibility that the trichohyalin protein is specifically (or preferentially) involved in aggregating intermediate filaments containing the K6/K16 keratins.

Animals↗
PubMed1994

Simple and efficient procedure for removing the 34 kDa allergenic soybean protein, Gly m I, from defatted soy milk.

The 34 kDa protein in the salted-out fraction of soy milk is shown to be identical to the allergenic soybean protein, Gly m I, by N-terminal amino acid sequencing and immunoblotting, using the monoclonal antibody against Gly m I. Treatment of soy milk with 1 M Na2SO4, acidifying to pH 4.5 and centrifugation removed more than 90% of the 34 kDa protein with an overall yield of 70% of total soy protein.

Allergens↗
PubMed1994

[Effect of dopamine administration on ICG-disappearance rate during prone position anesthesia].

We evaluated the effect of dopamine administration (3 micrograms.kg-1.min-1) on ICG-disappearance rate during prone position in 10 patients undergoing elective spinal cord surgery. Anesthesia was maintained with isoflurane and 50% nitrous oxide in oxygen. ICG-disappearance rate was determined at 3 points, i.e., the day before surgery, during prone position and during the administration of dopamine. Systolic arterial pressure showed no significant difference before and during dopamine administration. ICG-disappearance rate during prone position was significantly lower than the preoperative value. However, ICG-disappearance rate showed no significant difference during dopamine administration and preoperative period. We conclude that dopamine administration may attenuate the depressant effect of the prone position on the hepatic blood flow.

Adult↗
PubMed1994

[ICG-disappearance rate during sevoflurane- or neurolept anesthesia].

We evaluated the effect of sevoflurane or neuroleptanesthesia on ICG-disappearance rate in 22 patients undergoing elective extra-abdominal surgery. We divided the patients into three groups, i.e., SN-group (n = 8), SH-group (n = 8) and NLA-group (n = 6). Systolic arterial pressure (SAP) in the patients of SN-group or SH-group was maintained with sevoflurane at about 100% or 70% of the preoperative value respectively. There was no significant difference in SAP between SN-group and NLA-group. In SN-group and NLA-group, there was no significant difference in the ICG-disappearance rate between the values during anesthesia and those during preoperative period. In SH-group, however, it was significantly lower than the preoperative value. We conclude that, when normal systolic arterial pressure can be maintained, sevoflurane or neuroleptanesthesia has little effect on the liver function and hepatic blood flow. Sevoflurane anesthesia with 70% of preoperative SAP, however, may depress the liver function and hepatic blood flow.

Aged↗
PubMed1993

Lidocaine inhibits priming and protein tyrosine phosphorylation of human peripheral neutrophils.

The addition of agents, such as tumor necrosis factor-alpha, to human peripheral neutrophils (HPPMN) induces priming, which enhances the receptor-mediated superoxide (O2-) generation and tyrosine phosphorylation of several HPPMN proteins. Lidocaine, a local anesthetic, inhibited both enhanced O2- generation and tyrosine phosphorylation of a 115 kDa protein in a concentration- and time-dependent manner. Lidocaine also inhibited protein kinase C sensitive O2- generation induced by phorbol myristate acetate, but not time dependently. Furthermore, lidocaine inhibited O2- generation by non-primed HPPMN induced by formylmethionyl-leucyl-phenylalanine, but this inhibition needed a higher concentration of lidocaine compared with that of primed HPPMN. These results suggest that lidocaine inhibits the priming step of neutrophil activation and that it is linked to the inhibition of tyrosine phosphorylation of a 115 kDa protein.

Humans↗
PubMed1993

Effects of probucol and pravastatin on plasma lipids, activities of postheparin lipoprotein lipase, and lecithin cholesterol acyltransferase and apo A-I containing lipoproteins with and without apo A-II in patients with moderate hypercholesterolemia.

In this study, plasma HDL fractions were separated by ultracentrifugation and apo A-I containing lipoproteins (A-I Lp) were then isolated using anti-apo A-I immunoaffinity chromatography. The A-I Lp were further separated into two fractions with the use of anti-apo A-II immunoaffinity chromatography. One fraction, Lp A-I, contained apo A-I without apo A-II, while the other, Lp A-I/A-II, contained both apo A-I and apo A-II. These techniques were applied to investigate the changes in HDL apoprotein composition in hypercholesterolemic subjects treated with either probucol or pravastatin. Treatment with probucol (500 mg/day) or pravastatin (10 mg/day) reduced mean plasma total cholesterol concentrations by 24% (p < 0.01) and 16% (p < 0.05), respectively. Both drugs caused some reduction in lipoprotein lipase activity, but neither had any influence on the activity of hepatic triglyceride lipase or lecithin cholesterol acyltransferase. Their effects on HDL-cholesterol levels and apoprotein composition differed markedly. Probucol significantly decreased the HDL-cholesterol concentration, the plasma apo A-I/apo A-II ratio, and the number of large particles of diameter greater than 10.4 nm. When the ratios of Lp A-I and Lp A-I/A-II for the probucol-treated subjects were compared with those in the normolipidemic controls, and with the ratios before and after administration of probucol, a remarkable decrease in the level of Lp A-I was apparent. It is presumed that the decrease in HLD-cholesterol by prolonged probucol administration reflects the decrease of Lp A-I more than the decrease of Lp A-I/A-II.(ABSTRACT TRUNCATED AT 250 WORDS)

Apolipoprotein A-I↗
PubMed1993

Microinjection of alpha-calcitonin gene-related peptide into the hypothalamus activates sympathetic outflow in rats.

Effect of rat alpha-calcitonin gene-related peptide (alpha-CGRP) microinjected into various hypothalamic nuclei on plasma levels of catecholamines and arterial blood pressure were investigated in urethane-anesthetized rats. alpha-CGRP (0.05 and 0.25 nmol) microinjected into the hypothalamic paraventricular nucleus (PVN) increased the plasma level of noradrenaline (NA), but not that of adrenaline (AD), in a dose-dependent manner. A similar increase in plasma level of NA was also observed by alpha-CGRP (0.05 nmol) microinjected into the preoptic area (POA), anterior hypothalamus (AH), dorsomedial hypothalamus (DMH) and ventromedial hypothalamus (VMH). A significant increase in arterial blood pressure was observed by microinjection of alpha-CGRP (0.05 nmol) into the PVN, POA, AH and DMH, and the most prominent increase was caused by its microinjection into the PVN. Microinjection of the same dose of this peptide into the VMH, lateral hypothalamic area and posterior hypothalamus was without effect. The increase in plasma level of NA induced by alpha-CGRP (0.05 nmol) into the PVN was not affected by bilateral adrenalectomy. Electrical stimulation of the PVN elicited increases in plasma levels of both NA and AD. This increase in NA was abolished by chemical sympathectomy with 6-hydroxydopamine (50 mg/kg, i.v., 3 days before experiments). These results suggest that activation of the PVN by electrical stimulation elicits both sympathetic and adrenomedullary outflow. alpha-CGRP microinjected into the PVN selectively activates the sympathetic outflow.

Adrenalectomy↗
PubMed1993

[Effect of deliberate hypotension with PGE1 on PaO2 in pediatric patients].

We compared the effect of deliberate hypotension with PGE1 on PaO2 between pediatric and adult patients. Seven children, aged 3-9 yrs and 10 adults, aged 35-65 yrs who were scheduled for elective head and neck surgeries were studied. Anesthesia was maintained with enflurane, 50% N2O in oxygen and supplemental infusion of fentanyl. Ventilation was controlled to maintain PaCO2 at 35-40 mmHg. Hypotension was induced with continuous infusion of PGE1 and the systolic blood pressure was maintained at 70% of the presurgical value. Blood gases were measured three times, i.e. before, during, and after hypotension. The hypotension in adults caused a significant reduction in PaO2. In children, on the contrary, PaO2 was not affected by the hypotension. The results suggest that intrapulmonary shunting is smaller in children than in adults during PGE1-induced hypotension.

Adult↗
PubMed1993

[Effect of the prone position on ICG excretion during normotensive or hypotensive isoflurane anesthesia].

We evaluated the effect of prone position on ICG excretion during normotensive and hypotensive isoflurane anesthesia. In supine position, either normotensive or hypotensive anesthesia produced no significant prolongation in ICG excretion. In prone position, however, both normotensive and hypotensive anesthesia caused a significant prolongation in the excretion. The results suggest that prone position decreases the hepatic blood flow or hepatic function during isoflurane anesthesia.

Adult↗