Search PubMed⌕ Search

Biomedical subjects

M Majer

Publications and source records attributed to M Majer.

60 records · Page 4Linked to original sources
PubMedDate not supplied

Results of the single-radial-diffusion test with formaldehyde-treated influenza virus.

We found reduced values for formaldehyde-treated influenza virus in the single-radial-diffusion test by comparison with non-treated virus. The reduction correlates with the formaldehyde concentration. Hemagglutination and neuraminidase activities were not affected by the same treatment. We therefore suggest that this reduction is not based on antigen destruction.

Formaldehyde↗
PubMedDate not supplied

BCG-immunotherapy of L2C guinea pig leukemia.

In different experiments 46 guinea pigs of inbred strain 2 were inoculated intradermally with a lethal dose of about 10(5) gelatine purified L2C leukocytes per animal in mixture with 10(7) colony forming units (CFU) of BCG Copenhagen 1331. From these animals 58% showed a significant (p = 0.05) delay of death from leukemia in comparison to 38 animals not treated with BCG all perishing of leukemia with an average life time of 22.7 days (+/- 4.1 S.D.) after tumor cell inoculation. In 15% of the animals BCG caused long term survivors, still alive more than three months after L2C application. Twelve of them were challenged with nearly 10(5) L2C leukocytes; nine animals remained healthy demonstrating strong acquired resistance against L2C leukemia. Fresh submers cultured and lyophilized pellicle grown preparations of BCG Copenhagen 1331 were equally effective in antitumor activity. Using the same CFU dosage the lyophilized Copenhagen 1331 preparation was compared with two other commercial BCG vaccines and was found equivalent or better in efficacy against L2C leukemia.

Animals↗
PubMedDate not supplied

Interference of preceding BCG vaccination with BCG immunotherapy in L2C-guinea pig leukemia.

Guinea pigs of inbred strain 2 were vaccinated with BCG 1331 Copenhagen (K-Nr. 240-1) six to seven weeks before intradermal application of about 10(5) leukocytes from a highly leukemic animal in mixture with 10(7) colony forming units (CFU) of BCG. This presensitization with BCG totally abolished or strongly reduced the antitumor activity of L2C-cells adjoining BCG. Increase of bacterial mass applied from 1 X 10(7) to 2 X 10(8) CFU did not restore the antileukemic effect of BCG as observed in unvaccinated guinea pigs by a significant delay of death in a portion of animals and by the occurrence of long-term survivors. Since local reaction to BCG occurred much earlier in presensitized than in unvaccinated animals, we speculate that the destruction of tumor cells and hence the antigenic stimulus was too premature in presensitized animals to build up a BCG-mediated antitumor immunity.

Animals↗
PubMedDate not supplied

Zonal-centrifuged human diploid cell rabies vaccine.

In order to reduce the risk of allergic reactions, a purified human diploid cell culture rabies vaccine was developed. Pitman-Moore strain of rabies was propagated in WI-38 or MRC-5 cells. Both cell strains were found to be equally suitable for pro agation of rabies virus. The virus was purified and concentrated by continuous-flow sucrose density gradient ultracentrifugation. Virus peak fractions were diluted with a stabilized and then inactivated with BPL and freeze-dried. According to the standard NIH test for potency, the vaccine proved to be very potent and stable. The results of initial clinical applications of the vaccine will be presented.

Antibody Formation↗