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Biomedical subjects

M Macek

Publications and source records attributed to M Macek.

At least 145 records · Page 8Linked to original sources
PubMed1976

[Genetics].

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Adolescent↗
PubMed1975

Creatinephosphokinase in human diploid cell lines.

The activity of CPK was proved in cultivated cells from amniotic fluid cells, thymus, spleen, pericardium, umbilical cord, skin and muscle. The activity in these strains did not significantly differ. The average value is 224 plus or minus 68 i.u. CPK/mg N. No differences of activity were disclosed between embryonal, foetal and children cultures. The activity of CPK in post mortem cultures was not altered. The relationship between collagen synthesis and CPK activity is suggested. The evidence of CPK activity in amniotic fluid cells corresponding to the activity in other foetal cultures and the whole sample under investigation opens the way to the prenatal investigation of pathophysiology and inborn errors of the metabolism of this enzyme.

Amniotic Fluid↗
PubMed1975

Cytochemical and cytogenetic findings in five human leukocyte long-term cultures (LAHL) of different origin.

Five human leukocyte long-term suspension cultures were investigated by means of cytochemical and cytogenetic methods. A striking resemblance in the morphology of these cells originating either from peripheral blood or from spleen of patients with or without hematological disorders was found. Lymphocytic origin of all five cultures is suspected. In the three cultures a diploid karyotype was found with some aberrations. In one culture, derived from the spleen of a patient with panmyelopathy the mosaic 45 XX C -/46 XX was detected. Anomalies of C group chromosomes were the most consistent type of chromosome aberrations in two lines. Both were EBV positive.

Acid Phosphatase↗
PubMed1975

Biosynthesis of collagen during the life cycle of human diploid cell lines.

In the course of the active phase of growth collagen synthesis appears relatively stable and decreases when the culture passes into the degenerative phase III. The increase of cell nitrogen indicates the increasing accumulation of cell proteins with their aging in vitro. The ratio of soluble to insoluble collagen is relatively constant in fibroblast cultures from different tissues, periods of development during the active phase of growth. The increase of insoluble collagen in long term cultivated Marfan syndrome fibroblasts indicates the possible relationship between increasing stability of collagen and ageing of fibroblasts in vitro. Further studies confirmed the significant differences between control fibroblast and fibroblast derived from tissues of patients affected by Marfan syndrome. No differences were found in collagen synthesis and in the ratio of soluble to insoluble collagen in fibroblast strains from embryos, foetuses, children and young adults. The collagen synthesis and the ratio of soluble to insoluble collagen does not differ in fibroblast cultures derived from or post-natal period of life. The collagen synthesis in amniotic fluid cells was confirmed by analysis of cell proteins and proteins produced into the cultivation medium. The ultrastructural analysis of these cells reveals formation of intracellular fibrills and their growth in the extracellular space. The possibility of prenatal investigation of inborn errors of collagen metabolism is pointed out.

Adrenal Glands↗