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Biomedical subjects

M Müller

Publications and source records attributed to M Müller.

At least 1,081 records · Page 60Linked to original sources
PubMed1991

Effect of intraoperative aprotinin administration on postoperative bleeding in patients undergoing cardiopulmonary bypass operation.

To study the hemostyptic effect of aprotinin (Trasylol) in patients undergoing extracorporeal circulation for coronary artery bypass operations, we randomized 12 of 24 patients to receive aprotinin in high dosage (about 800 mg) during extracorporeal circulation. From the resulting two groups each, one patient was excluded from the study because of postoperative myocardial infarction (control group) and surgical hemorrhage (aprotinin group) leading to a second operation. Although heparin was used for anticoagulation in all 22 patients, all had a marked increase in plasma levels of thrombin-antithrombin III complexes during extracorporeal circulation, indicating an intravasal activation of coagulation. By monitoring the plasma levels of fibrin degradation products in patients without aprotinin therapy, we recorded a concomitant hyperfibrinolysis significantly less pronounced in patients receiving aprotinin (p less than 0.005). The mean total postoperative blood loss was lower in patients receiving aprotinin (620 ml) than in control patients (1000 ml; p less than 0.03). The results confirm previous reports of a hemostyptic effect of aprotinin in cardiac operations. This effect is probably due to a prevention of hyperfibrinolysis.

Antithrombin III↗
PubMed1991

Quantification of ATP-producing and consuming processes in quiescent pig spleen lymphocytes.

ATP production in quiescent pig spleen lymphocytes was estimated on the basis of their coupled respiration. ATP-consuming processes were assessed from the effects of inhibitors of protein synthesis, proteolysis, RNA synthesis, Na+ K(+)-ATPase and Ca(2+)-ATPase on respiration. About 95% of the total ATP consumption could be assigned to specific processes. More than 50% of the ATP produced appear to be consumed by the cation transport ATPases.

Adenosine Triphosphate↗
PubMed1991

[The taxonomy of the genera of Alternaria (Nees: Fr)].

The identification of species in Alternaria (Nees:Fr.) is mainly based on shape, size and septation of conidia. Because of the great variances in these marks a reliable determination of species in that genus sometimes is difficult. 137 strains of Alternaria isolated from forage are attempted to identify. Various macro- and micromorphological methods used are described as well as their sources of errors. It is emphasized that a stringent standardization of all determination methods is necessary. Beyond it there are discussed some molecular biological and biochemical characteristics to complete the data of taxonomy in the genus of Alternaria.

Alternaria↗
PubMed1991

[Alternaria infestation of corn silage and hay].

The numbers of fungi and the portions of Alternaria species in silage maize before harvesting and in hay stored were determined. Alternaria is present in growing maize with 18.3 percent of the total numbers of fungi. It occurs mainly on leaves and husks. Fungi of the genus Alternaria also were found in hay, that has been stored for 8 months when total numbers of fungi have been decreased slowly. More than 80 percent of all Alternaria strains isolated were determined systematically as Alternaria alternata Fries (Keissler) and A. tenuissima (Kunze:Fr.) Wiltshire. Based on these results there is discussed the potential hazard of a contamination of the roughages with Alternaria mycotoxins.

Alternaria↗
PubMed1991

Photoaffinity labeling of leukotriene binding sites in hepatocytes and hepatoma cells.

The method of direct photoaffinity labeling in the frozen state using the leukotrienes as suitable photolabile compounds may serve to identify and characterize polypeptides which interact with these eicosanoids during their hepatobiliary transport and metabolism. Furthermore, it will be a helpful technique to evaluate changes in the cell-specific protein pattern during neoplastic dedifferentiation of hepatocytes.

Affinity Labels↗
PubMed1991

Ornithine decarboxylase immunoreactivity in the pituitary gland. A comparative lightmicroscopical study.

In the present study efforts are made to localize ornithine decarboxylase enzyme protein--the key enzyme of polyamine biosynthesis--in the adenohypophysis of different vertebrates by means of immunocytochemistry. The antigenic expression of ornithine decarboxylase was revealed in the pituitary of the clawed frog (Xenopus laevis D.), but not in rat and human adenohypophysis. The immunocytochemical results are compared with the staining pattern of the periodic acid-Schiff-reaction. No correlation between these results and the immunocytochemically obtained data has been found. Conclusions are drawn from the location of the enzyme and possible phylogenetic and humoral regulation mechanism.

Animals↗
PubMed1991

[Double stapling suture anastomosis in deep anterior rectum resection. Experiences and results].

Between 1.1. 1984-31.12. 1989, 263 rectal carcinomas were operated at the Department of Surgery, St. Veit/Glan. The resection rate was 68.4%, the excision rate was 27.8%, other procedures were carried out in 3.8%. Staple devices were used for all anastomoses following rectum resection. Whenever technically possible, the single-stapled procedure was used (EEA, ILS). For difficult or very low anastomoses, the double-stapled technique (DST) was utilized. The DST was employed in 47 cases. This approach contributed essentially to a relatively high resection rate at our department.

Adult↗
PubMed1991

[Unexpected transgene expression of a mammary-specific growth hormone gene construct in Bergmann glial cells of the mouse].

Pronuclear microinjection was used to produce transgenic mice harboring gene constructs, in which 110 base pairs (WAP1) or 2.4 kilobases (WAP2) of the 5' flanking sequences of the whey acidic protein (WAP) gene were fused to human growth hormone (hGH)-coding sequences. Female WAP-hGH transgenic mice expressed the transgenes in the mammary gland, the expression of the WAP2-hGH transgene mirroring that of the endogenous WAP gene. When other organs were examined, high level expression of hGH was observed in the brains of WAP2-hGH transgenic mice. Using in situ hybridization and immunohistochemistry, hGH expression from the transgene was seen to occur specifically in Bergmann glia cells. While normally neither WAP nor hGH is expressed in this type of cell, it appears that a combination of the regulatory region of the WAP gene and the hGH structural gene results in a novel tissue specificity in Bergmann glia cells.

Animals↗
PubMed1991

[Abdominal lymphangioma in childhood].

The symptoms of abdominal lymphangioma are unspecific and depend on site and size of the tumor. The most frequent symptoms consist of pain up to the clinical picture of a pseudo-appendicitis, digestive problems, palpable or even exterior visible tumors such as the pseudo-ascites. Often the diagnosis cannot be found by clinical examination or further investigation. As a differential diagnosis all mesenterial cysts as well as other cystic tumors which originate in an abdominal organ must be taken in account. In most cases possible complications make surgical intervention necessary through which an exact diagnosis of the actually asymptomatic tumor can be found. Lymphangioma can lead to obstruction of the intestine, the ureter or the bile-duct. Inflammations of the cyst wall, bleeding into the cyst, torsion of the cyst pedicle and rupture of the cyst occur. The only curative therapy is the total surgical removal. Prognosis is good and relapse seldom occurs. We report on a 4.5-year-old boy with a cystic lymphangioma of the mesosigma. In addition, we discuss cases from the literature of the past 15 years.

Abdomen, Acute↗
PubMed1990

ATP-dependent primary active transport of cysteinyl leukotrienes across liver canalicular membrane. Role of the ATP-dependent transport system for glutathione S-conjugates.

The liver is the major organ which eliminates leukotriene C4 (LTC4) and other cysteinyl leukotrienes from the blood circulation into bile. Transport of LTC4 was studied using inside-out vesicles enriched in canalicular and sinusoidal membranes from rat liver. The incubation of canalicular membrane vesicles with [3H]LTC4 in the presence of ATP resulted in an uptake of LTC4 into vesicles. The initial rate of ATP-stimulated LTC4 uptake was about 40-fold higher in canalicular than in sinusoidal membrane vesicles. When liver plasma membrane vesicles were incubated in the absence of ATP, an apparent transient uptake of LTC4 was observed which was temperature-dependent and not affected by the osmolarity. This indicates that LTC4 was bound to proteins on the surface of plasma membrane vesicles. Two proteins with relative molecular weights of 17,000 and 25,000 were detected by direct photoaffinity labeling as major LTC4-binding proteins. One protein (Mr 25,000) was ascribed to subunit 1 (Ya) of glutathione S-transferase which was associated with the membrane. LTD4, LTE4, N-acetyl-LTE4, and omega-carboxy-N-acetyl-LTE4 were also transported into liver plasma membrane vesicles in an ATP-dependent manner with initial rates relative to LTC4 (1.0) of 0.46, 0.11, 0.35, and 0.22, respectively. Mutual competition between the cysteinyl leukotrienes and S-(2,4-dinitrophenyl)-glutathione for uptake indicated that they are transported by a common carrier. Apparent Km values of the transport system for LTC4, LTD4, and N-acetyl-LTE4 were 0.25, 1.5, and 5.2 microM, respectively. The ATP-dependent transport of LTC4 into vesicles was not inhibited by doxorubicin, daunorubicin, or verapamil, or by the monoclonal antibody C219, suggesting that the transport system differs from P-glycoprotein. Liver plasma membrane vesicles prepared from mutant rats deficient in the hepatobiliary excretion of cysteinyl leukotrienes lacked the ATP-dependent transport of cysteinyl leukotrienes and S-(2,4-dinitrophenyl)-glutathione. These results demonstrate that the ATP-dependent carrier system is responsible for the transport of cysteinyl leukotrienes and glutathione S-conjugates from the hepatocytes into bile.

Adenosine Triphosphate↗
PubMed1990

A protein with sequence identity to Skp (FirA) supports protein translocation into plasma membrane vesicles of Escherichia coli.

We have purified to homogeneity a 15 kDa-protein from a ribosomal salt extract of Escherichia coli that compensates in vitro a defect of SecA but not of SecB. Removal of this protein from a cell-free transcription/translation system impairs translocation into plasma membrane vesicles of the precursors of LamB and to a lesser degree also of OmpA. These results suggest a role of the 15 kDa-protein in bacterial protein export. The NH2-terminal 35 amino acids were found to be identical to those of the skp (firA) gene product, to which several putative functions have previously been attributed.

Amino Acid Sequence↗
PubMed1990

Cytochrome c oxidases: polypeptide composition, role of subunits, and location of active metal centers.

The general structure of the enzyme, its polypeptide composition, and a proposal for a rational nomenclature are discussed. The mitochondrially coded and bacterial cytochrome c oxidase subunits have been analyzed with more attention focused on elucidating the number of metals present in the enzyme and the ligands available for their coordination. The picture of a 2 Cu/2 Fe enzyme has been compared with that of a 3 Cu/2 Fe enzyme and a new model is proposed for the location of the metal centers in the enzyme.

Amino Acid Sequence↗
PubMed1990

Determination of the three-dimensional structure of the Antennapedia homeodomain from Drosophila in solution by 1H nuclear magnetic resonance spectroscopy.

The determination of the three-dimensional structure of the Antennapedia homeodomain from Drosophila in solution is described. The techniques used are 1H nuclear magnetic resonance spectroscopy for the data collection, and calculation of the protein structure with the program DISMAN followed by restrained energy minimization with a modified version of the program AMBER. A group of 19 conformers characterizes a well-defined structure for residues 7 to 59, with an average root-mean-square distance from the backbone atoms of 0.6 A relative to the mean of the 19 structures. The structure contains a helix from residues 10 to 21, a helix-turn-helix motif from residues 28 to 52, which is similar to those reported for several prokaryotic repressor proteins, and a somewhat flexible fourth helix from residues 53 to 59, which essentially forms an extension of the presumed recognition helix, residues 42 to 52. The helices enclose a structurally well-defined molecular core of hydrophobic amino acid side-chains.

Amino Acid Sequence↗