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Biomedical subjects

M M Thaler

Publications and source records attributed to M M Thaler.

At least 73 records · Page 4Linked to original sources
PubMed1976

Hyperfibrinogenemia and polycythemia with intrauterine growth retardation in fetal lambs.

Plasma concentrations of albumin and fibrinogen and arterial hematocrits were determined during the last third of gestation in growth-retarded and control fetal lambs. The mean fetal plasma albumin concentration increased slightly as term approached and was not significantly different in the two groups. The mean plasma fibrinogen concentration did not change in the control fetuses, but was significantly elevated in the growth-retarded fetuses, as was the mean arterial hematocrit. The theoretical implications of these findings relative to capillary blood flow are discussed.

Animals↗
PubMed1976

Free fatty acids and albumin as mediators of thrombin-stimulated fibrinogen synthesis.

Mobilization of FFA in mice, triggered with an injection of thrombin, was followed within 24 h by a 2.5-fold increase in fibrinogen synthesis and a 30% increase in plasma fibrinogen concentration. In mouse liver slices, incubated in plasma, additions of palmitate or stearate in amounts similar to those expected in vivo in FFA mobilization stimulated fibrinogen synthesis 5.6- to 6.1-fold while unsaturated and short-chain FFA were less effective. Palmitate and linoleate also augmented albumin synthesis although not as stongly as fibrinogen synthesis. These observations raised the possibility that the greater effectiveness of saturated FFA in stimulating fibrinogen synthesis may reflect higher FFA/albumin ratios within hepatocytes in the presence of saturated FFA. Injection of exogenous defatted albumin into mice before thrombin injection prevented the FFA-associated rise in fibrinogen synthesis and plasma concentration. In incubated liver slices, defatted albumin abolished the FFA stimulation of fibrinogen synthesis when FFA/albumin ratios were maintained to the physiological range. These studies indicate that the FFA/ALBUMIN RATIO MAY PLAY A MAJOR ROLE IN THE REPLENISHMENT OF FIBRINOGEN AFTER PERIODS OF RAPID DEFIBRINOGENATION.

Albumins↗
PubMed1976

Serum protein synthesis in mutant mice with abnormal hepatic endoplasmic reticulum.

Severe ultrastructural abnormalities of liver endoplasmic reticulum have been described in newborn mice homozygous for radiation-induced deletion alleles at the colour locus. The ultrastructural defects were accompanied by deficiencies of several enzymes and lowered serum protein levels. Studies on serum protein synthesis were undertaken to see if decreased rates of synthesis, especially of constituents thought to be synthesized on membrane-bound ribosomes, were the cause of the deficiencies. Although decreases or absence of several serum proteins were shown, radiopulse-immunoprecipitation studies of albumin and fibrinogen synthesis suggested that the decreased synthesis rates were a secondary defect. Serum glycoproteins were not altered more than other constituents in the mutant material.

Albinism↗
PubMed1975

Purification of growth-promoting peptides and proteins, and of histones, by high pressure silica gel chromatography.

A rapid method for the purification of histones and a variety of growth-promoting proteins and peptides by chromatography on silica gel has been developed. The isolation of the growth-promoting components of serum has been hampered by excessive losses associated with the use of water-based purification mens in acidic methanol-H2O solutions (eg. insulin, albumin, the somatomedins) provides a basis for purification on high-pressure silica gel columns, while peptides and histones can be purified in similar solvents. After column chromatography, the solvent is removed by flash-evaporation, or the protein may be precipitated directly from the solvent by neutralization of the pH and the addition of ethanol. The retention of biological activity (eg. somatomedin-C binding to insulin receptors and cell-growth stimulation) and recovery are excellent.

Chromatography, High Pressure Liquid↗
PubMed1975

Reversibility of extensive liver damage in galactosemia.

An infant with galactosemia is reported in whom extensive liver damage developed by 1 month of age. Liver biopsy obtained prior to treatment indicated extensive periportal and intralobular fibrosis, ductular cysplasia. "pseudoglandular" transformation, and distortion of periportal vasculature. Three months after institution of a galactose-free diet, clinical and biological evidence of liver disease disappeared, and follow-up biopsy at 5 months of age showed normal hepatic histology. These findings demonstrate that functional and histological abnormalities consistent with cirrhosis can be completely reversed by dietary management in galactosemia.

Biopsy, Needle↗