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Biomedical subjects

M M Rapport

Publications and source records attributed to M M Rapport.

At least 55 records · Page 3Linked to original sources
PubMed1977

Identification of glial fibrillary acidic protein by the immunoperoxidase method in human brain tumors.

The immunocytochemical localization of glial fibrillary acidic portein within glioma cell bodies and their processes by the immunoperoxidase method is demonstrated to be of diagnostic value. This method has advantages over "special" stains because it is not so dependent upon color alone, and because it identifies a specific protein in the cells. The immunoperoxidase method using antiserum to glial fibrillary acidic protein is shown to be useful for the differentiation of mixed glial and mesenchymal tumors, and for the diagnosis of tumors in which a glial or mesenchymal cell origin is in doubt.

Brain Neoplasms↗
PubMed1976

Antiserum to brain gangliosides produces recurrent epileptiform activity.

A single injection of 10 microliters of antiserum to total brain ganglioside onto (and into) the sensorimotor cortex of the rat resulted in recurrent spiking in the cortical electroencephalogram, lasting from 7 to 17 days. Absorption of antibody with pure monosialoganglioside (GM1) completely abosished the effect. Spiking was reactivated after 4 weeks by intramuscular injection of pentylenetetrazole.

Action Potentials↗
PubMed1976

Effects of antisera to S-100 protein and to synaptic membrane fraction on maze performance and EEG.

Topical cortical injection into rats of antiserum to the synaptic membrane fraction caused recurrent spiking activity as observed in the EEG; neither antiserum to S-100 protein nor antiserum to myelin caused any such abnormality. These same rats, when observed on a maze learning task (Lashley III) responded differently to the different antisera. Rats receiving injections of antiserum to the synaptic membrane fraction or antiserum to S-100 protein were inhibited in their performance, while rats injected with antiserum to myelin behaved like uninjected animals.

Animals↗
PubMed1976

Isolation of a novel lipid hapten, cytolipin S, from rat spleen.

Antisera against rat erythrocytes contain agglutinins directed against unknown lipid determinants. Complement-fixation shows more reactivity with lipid extracts of rat spleen than of other rat tissues. The isolation of the reactive lipid from rat spleen, cytolipin S, is described. Cytolipin S is a glycosphingolipid containing glucose, galactose, and galactosamine with molar ratios of 1:2:1. It migrates on TLC like asialo GM1 (more slowly than cytolipin R, a ceramide tetrasaccharide, or cytolipin F, a ceramide pentasaccharide). Asialo GM1 and cytolipin S, when properly combined with auxiliary lipids, react very similarly with anti-rat erythrocyte sera by complement-fixation. However, cytolipin S is much more effective than asialo GM1 in inhibiting the hemagglutination reactions. It is concluded that cytolipin S and asialo GM1 are ceramide tetrasaccharides having different chemical structures and that the structural differences probably will be found in the carbohydrate linkages other than that between the terminal and penultimate residues.

Animals↗
PubMed1976

Is the serotonin binding protein (SBP) a soluble storage form for serotonin?

The binding of serotonin to a soluble, high affinity binding protein (SBP) present in synaptosomes and assoicated with serotonergic tracts, has now been studied for the effect of drugs and, in particular, drugs interacting with contractile proteins. Vincristine, vinblastine, and cytochalasin B were found to cause 50% inhibition of serotonin binding to SBP at 1.5 X 10(-6)M, 7.5 X 10(-6)M and 50 X 10(-6)M, respectively. Colchicine did not affect the binding at 1 X 10(-3)M. When vinblastine was injected intraventricularly, the binding capacity of SBP isolated from brain 20 and 26 hours after injection was decreased 42% and 60% respectively. It is concluded that SBP is an actin-like contractile protein, distinct from microtubulin, and not associated with membranes. The storage of serotonin through binding to a protein with such properties suggests that this compartment is in some way involved in the translocation or transfer of serotonin from one part of the cell to another.

Animals↗
PubMed1975

Behavioral changes in 2-month-old rats following prenatal exposure to antibodies against synaptic membranes.

Two-month-old male offspring of rats injected intravenously on day 19 of gestation with antiserum to synaptic membrane fraction showed marked behavioral deficits on a DRL (differential reinforcement at low rates) training paradigm. These behavioral deficits included perseveration, slow acquisition rates, and poor retention. Offspring of pregnant rats injected with antiserum to galactocerebroside or isotonic saline did not show these behavioral effects.

Animals↗
PubMed1975

Immunochemical studies of organ and tumor lipids XXI. Sensitivity and specificity of the cytolipin F - sheep erythrocyte system.

The sensitivity and specificity of the sheep erythrocyte - anti-sheep erythrocyte system to inhibition by pure cytolipin F has been studied with 5 antisera, in order to compare it with the rat erythrocyte-anti-rat lymphosarcoma system and its inhibition by pure cytolipin R. The cytolipin F - sheep erythrocyte system is much more sensitive than the cytolipin R - rat erythrocyte system, inhibition of hemolysis of 6 x 10(6) sheep cells being produced by 10 ng of cytolipin F (combined with a four-fold quantity of lecithin) compared with inhibition of hemolysis of 10(6) rat cells by 50 to 100 ng of cytolipin R (also combined with lecithin). Differences in sensitivity are attributed to the larger number of available cytolipin F determinants on sheep erythrocytes compared with cytolipin R determinants on rat erythrocytes. Studies of the auxiliary lipid enhancement of cytolipin F activity by galactocerebroside, lactosyl ceramide (cytolipin H), and lecithin are also reported.

Animals↗