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Biomedical subjects

M M Gupta

Publications and source records attributed to M M Gupta.

115 records · Page 7Linked to original sources
PubMedDate not supplied

PISA: a noninvasive method in detection and quantification of acid-induced myocardial infarction in dogs.

Phase invariant signature algorithm (PISA), a new noninvasive technique, was used in the detection and quantification of acid-induced myocardial damage in anesthetized dogs. The diagnostic capabilities of this method were compared with those of conventional electrocardiogram and biochemical markers, MB-creatine phosphokinase (MB-CPK) and lactic dehydrogenase (LDH1). Myocardial damage of varying degree was induced by injecting diluted sulphuric acid (0.01 to 0.10 ml) into the free wall of the left ventricle. Conventional ECG, wideband ECG for PISA analysis, blood samples for LDH1, and MB-CPK were taken before and after 15, 30, 60, and 90 min of acid injection. The heart was removed at the end of 90 min for estimation of myocardial damage. PISA Index increased within 10-15 min of acid injection and remained elevated for the duration of the experiment (90 min). The increase in the PISA index was directly related to the extent of myocardial damage and the amount of acid injected. Although the conventional electrocardiogram detected large myocardial damage, it was unable to detect small myocardial damage. Also, most of initial change in conventional ECG with large myocardial damage disappeared within 90 min, while the PISA index was still elevated to the maximum level. The MB-CPK was not detected before or after myocardial damage. There was no significant change in the LDH1 at any time after myocardial infarction. These results suggest that the PISA technique is superior to the conventional ECG and the biochemical markers and would be a valuable diagnostic tool in the detection and quantification of incipient as well as advanced myocardial infarction.

Animals↗
PubMedDate not supplied

Quantitative determination of oleane derivatives in Terminalia arjuna by high performance thin layer chromatography.

A simple, precise and rapid high performance thin layer chromatographic method has been developed for the simultaneous quantitative determination of five oleane derivatives, namely, arjunic acid, arjunolic acid, arjungenin, arjunetin and arjunglucoside I from stem bark extract of Terminalia arjuna. The isolation and separation of these compounds was carried out on 60F254 layers eluted with chloroform:methanol (90:10), and the analytes were visualised through colour development with vanillin in concentrated sulphuric acid:ethanol. Scanning and quantification of the spots at 640 nm showed good recoveries in the range 96.40-101.7%.

Calibration↗
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Association between reproductive tract infections and cervical inflammatory epithelial changes.

OBJECTIVE: To determine the association, if any, between different reproductive tract infections and cervical inflammatory epithelial changes. STUDY DESIGN: Clinical, colposcopic, cytologic, and microbiologic screening for reproductive tract infections was conducted. Subjects were screened for Chlamydia trachomatis, Neisseria. gonorrhoeae, Trichomonas vaginalis, bacterial vaginosis, yeast vaginitis, human papillomavirus, and serology for herpes simplex viruses, syphilis, and human immunodeficiency virus-1 and -2. The subjects were 257 women who visited a maternal and child health center between January 1992 and December 1993. RESULTS: Cervical cytology revealed inflammatory epithelial changes in 207 women (80.5%), the highest proportion in the published series. Inflammatory epithelial changes were significantly associated with the number of reproductive tract infections. Risk increased up to 72.6 fold when women were infected with two or more agents. The positive predictive value of inflammatory epithelial changes for any infection was 88.4%, whereas the negative predictive value for any infection was 63.4%. In univariate analysis, the cervical inflammatory epithelial changes were significantly associated with infections such as chlamydia (28.0%), human papillomavirus (56.5%), Trichomonas vaginalis (18.3%), bacterial vaginosis (38.2%), and herpes simplex virus as evidenced by the presence of immunoglobulin M antibodies (28%). Multivariate analysis, however, revealed an independent association of inflammatory epithelial changes with chlamydia (odds ratio, 21.3; 95% confidence interval, 2.6, 181.3), human papillomavirus (odds ratio, 13.5; 95% confidence interval, 4.5, 39.6), and bacterial vaginosis (odds ratio, 22.6; 95% confidence interval, 2.9, 181) only. CONCLUSION: Cervical inflammatory epithelial changes are significantly associated with reproductive tract infection. Cervical inflammatory epithelial changes predict correctly to an extent of 81% infections with chlamydia, bacterial vaginosis, and human papillomavirus infection. A negative smear, on the other hand, rules out these infections to an extent of 83%.

Adult↗
PubMedDate not supplied

Determination of 1,3-benzodioxanes in Piper mullesua by high-performance thin-layer chromatography.

A new, simple, precise, and rapid high-performance thin-layer chromatographic method was developed for the determination of 6 benzodioxanes in Piper mullesua extract: 1',3'-benzodioxole-5'-(2,4,8-triene-isobutyl nonanoate), 1',3'-benzodioxole-5'-(2,4,12-triene-isobutyl tridecanoate), fargesin, sesamin, asarinin, 1',3'-benzodioxole-5'-(2,4,8-triene-methyl nonanoate). The ingredients were separated on a precoated Silica Gel 60 F254 plate with a solvent system of toluene-acetone (92 + 8). The 6 benzodioxanes were well separated and easily identified in this chromatographic system. The separated benzodioxanes were visualized by color development with a spray reagent consisting of 1 g vanillin dissolved in 100 mL H2SO4-ethanol (5 + 95, v/v). Quantitation was performed by scanning the spots and comparing the integrated areas of compounds in samples with those of standards. Recoveries from samples spiked with known amounts of the benzodioxanes were excellent. The results were comparable with those estimated by liquid chromatography.

Chromatography, Liquid↗
PubMedDate not supplied

PISA--a noninvasive method in the evaluation of protective effect of methylprednisolone on the ischemic myocardium.

This study deals with the assessment of the PISA--a new noninvasive method, in relation to conventional ECG, MBCPK and LDH1, in the detection and quantification of coronary ligation-induced myocardial damage. Also this study includes the applicability of the PISA method in the evaluation of the effectiveness of methylprednisolone in the protection of myocardial damage, induced by coronary ligation. The studies were conducted in 15 mongrel dogs: (untreated dogs: 9; methylprednisolone (30 mg/kg I.V.) treated dogs: 6). The PISA signature pattern was normal and the PISA index was small in all the dogs before coronary ligation. The PISA signature was phase-variant in all dogs in the untreated group following coronary ligation. The PISA index increased with time following coronary ligation but the changes at 60 minutes were less than at 30 and 120 minutes. There were no significant changes in the conventional ECG, serum MBCPK and LDH1 for the 120 minute duration of coronary ligation. These results indicate the superiority of the PISA method in the detection and quantification of coronary artery ligation-induced myocardial damage. Coronary ligation in methylprednisolone treated dogs produced significant increases in the PISA index with the time but the increases were significantly lower than those in untreated groups. These lower increases at 60 minutes as compared to 30 and 120 minutes observed in the untreated group were absent in the treated group. Methylprednisolone showed some protective effect against coronary ligation-induced myocardial damage. These results indicate that the PISA method may be a valuable tool in the detection and the measurement of the extent of cardiac injury and in constant monitoring of the changes in the size of myocardial damage.

Animals↗