Before or after treatment with sulfur mustard, simple changes in the incubation conditions of cultured Chinese hamster cells lead to changes as great as a factor of 6 in survival-curve slopes. With x-ray treatment, changes are similar but much smaller in magnitude. These results suggest that the modes of action of these agents are not entirely the same.

Animals↗

PubMed1967

Radiation response of mammalian cells grown in culture. VI. Protein, DNA, and RNA inhibition during the repair of x-ray damage.

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Cell Division↗

PubMedDate not supplied

Sublethal and potentially lethal damage repair on thermal neutron capture therapy.

Tonicity shock or caffeine postirradiation treatment makes evident fast-type potentially lethal damage (PLD). Caffeine expresses fast-type PLD more efficiently than tonicity shock in X-irradiated B-16 mouse melanoma cells, compared with V79 Chinese hamster cells. The survival curves of thermal neutrons for either V79 or B-16 cells exhibit no shoulder. Neither V79 nor B-16 cells show the sublethal damage (SLD) repair of thermal neutrons. Caffeine-sensitive fast-type PLD repairs exist in X-irradiated B-16 cells, as well as V79 cells. The fast-type PLD repair of B-16 cells exposed to thermal neutrons alone is rather less than that of X-irradiated cells. Furthermore, an extremely low level of fast-type PLD repair of B-16 cells with 10B1-paraboronophenylalanine (BPA) preincubation (20 hours) followed by thermal neutron irradiation indicated that 10B(n,alpha)7Li reaction effectively eradicates actively growing melanoma cells. The plateau-phase B-16 cells are well able to repair the slow-type PLD of X-rays. However, cells can not repair the slow-type PLD induced by thermal neutron irradiation with or without 10B1-BPA preincubation. These results suggest that thermal neutron capture therapy can effectively kill radioresistant melanoma cells in both proliferating and quiescent phases.

Animals↗