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M M Burger

Publications and source records attributed to M M Burger.

188 records · Page 11Linked to original sources
PubMed1969

Surface-specific characteristics of a contact-inhibited cell line containing the SV40 viral genome.

A cytoagglutinin purified from wheat germ lipase agglutinated five related murine fibroblast cell lines in the order of their saturation densities. One cell line, which was transformed by the oncogenic papova virus SV40 but which had a low saturation density, agglutinated poorly. Preincubation of cells with trypsin increased their agglutinability, but trypsin made no cell line more agglutinable than the cell line with the highest saturation density.

Agglutination Tests↗
PubMedDate not supplied

Diacylglycerol in large alpha-actinin/actin complexes and in the cytoskeleton of activated platelets.

The interaction of the cytoskeleton with plasma membranes may be mediated by vinculin, alpha-actinin and other proteins; alpha-actinin can interact specifically with model membranes only if they contain diacylglycerol and palmitic acid. On stimulation of platelets by thrombin, which leads to a reorganization of the cytoskeleton, diacylglycerol is produced rapidly, simultaneously with the disappearance of phosphatidylinositol. One important function of the diacylglycerol produced in platelets may be the activation of the Ca2+-and phospholipid-dependent protein kinase C. We show here that, in the presence of diacylglycerol and palmitic acid, a supramolecular complex between alpha-actinin and actin is formed in vitro. In the electron microscope, this complex displays substructures similar to those of microfilament bundles in vivo. Furthermore, such alpha-actinin/lipid complexes can also be formed in situ during the stimulation of blood platelet aggregation. Thus, alpha-actinin may be one of the proteins directly involved in structures connecting the cytoskeleton to cell membranes.

Actinin↗