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M M Berman

Publications and source records attributed to M M Berman.

7 recordsLinked to original sources
PubMed1991

Immunocytochemical identification of Helicobacter pylori in formalin-fixed gastric biopsies.

H&E and special histochemical stains are used by most laboratories to identify Helicobacter pylori (H. pylori) in gastric biopsy specimens. However, background staining can complicate recognition of H. pylori and small numbers of organisms may be overlooked. Additionally, histochemical stains do not distinguish H. pylori from other spiral organisms. We investigated two commercially available monoclonal antibodies, one directed against Campylobacter coli and C. jejuni (MAB002) and the other against a Campylobacter species flagellar antigen (MAB001), to evaluate potential use in immunocytochemical examinations of fixed tissues. MAB002 reacted with C. jejuni but not H. pylori organisms. MAB001 labeled C. jejuni as well as H. pylori and, therefore, was used to study 220 gastric biopsies from patients undergoing endoscopy. Acute and/or chronic gastritis was present in 60.5% (133/220) of the biopsies examined. MAB001 positivity was identified in 62.4% (83/133) of the tissues with gastritis. Only 2 of 87 (2.3%) specimens without gastritis demonstrated MAB001 labeling. The resulting immunoreactivity was easily identified, allowing specimens to be screened quickly and accurately. No labeling was seen with the non-Helicobacter/Campylobacter bacteria or normal tissues evaluated in this investigation. MAB001 can be used to identify H. pylori in histologically processed tissue and will assist pathologists, clinicians, and researchers studying the distribution and pathogenicity of this organism in humans and animals.

Antibodies, Bacterial↗
PubMed1991

The ectopic ovary. A case report and review of the literature.

We describe an ectopic ovary in a stillborn female. To our knowledge, this is the first report of an extra ovary in an infant. This case prompted a review of ectopic ovarian tissue, which is known by a variety of terms, the most common being accessory ovary and supernumerary ovary. We suggest that (1) many of the past cases should be classified as ovarian implants rather than true embryologically derived ectopic tissue; and (2) the terms accessory ovary and supernumerary ovary are imprecise and should be modified.

Choristoma↗
PubMed1990

Identification of human immunodeficiency virus in the heart of a patient with acquired immunodeficiency syndrome.

A 35-year-old homosexual white male diagnosed as having human immunodeficiency virus (HIV) infection presented with recurrent pericardial effusion, and HIV was isolated from the pericardial fluid. Autopsy revealed an extensive gross infiltrative lesion in the left ventricle of the heart. Microscopic examination of the myocardium demonstrated multifocal atypical lymphoid cell infiltrations associated with scattered foci of myocardial necrosis. The atypical lymphoid cells were immunoreactive to UCHL-1, a T cell antigen antibody. HIV was immunocytochemically identified in the cardiac lymphocytic infiltrate with anti-p24 monoclonal antibodies. This finding was supported by ultrastructural examination and in situ hybridization study.

Acquired Immunodeficiency Syndrome↗
PubMed1987

Drug-induced left ventricular failure in patients with pulmonary disease. Endomyocardial biopsy demonstration of catecholamine myocarditis.

Three patients with severe chronic lung disease had left ventricular failure develop with marked impairment of cardiac function. Ejection fractions by radioactive blood pool ventriculography were 0.17, 0.24, and 0.20. Right ventricular endomyocardial biopsy specimens showed interstitial hemorrhage and foci of interstitial polymorphonuclear leukocytes, strongly suggestive of catecholamine myocarditis. These patients had used beta-adrenergic agonist inhalants and methylxanthines. One of them clearly abused the inhalant and had elevated levels of urinary catecholamines. Progressive deterioration of pulmonary and cardiac function occurred in two patients, with death within three months of the initial myocardial biopsy. Concomitant use of beta-adrenergic agonists and methylxanthines may cause myocarditis with left ventricular failure in susceptible patients.

Adrenergic beta-Agonists↗