Search PubMed⌕ Search

Biomedical subjects

M Luciak

Publications and source records attributed to M Luciak.

At least 19 recordsLinked to original sources
PubMed1995

Myoinositol incorporation into lymphocytes of chronic renal failure patients is impaired.

Incorporation of myo-[2-3H]-inositol into peripheral blood mononuclear cells (PBMNC) and T-cell enriched lymphocytes was evaluated in in-vitro experiments in chronic renal failure (CRF) patients and healthy subjects. Incorporation of myo-[2-3H]-inositol into the cells of CRF patients on conservative and haemodialysis treatment was found to be impaired in comparison with that observed in normal cells. Following PHA stimulation of the cells of CRF patients myo-[2-3H]-inositol incorporation decreased even further, while it increased in normal cells. Five-hour haemodialysis session significantly depressed myoinositol incorporation into PBMNC, while its incorporation into T-cell enriched lymphocytes remained unaffected. Myoinositol incorporation into PBMNC and T-cell enriched lymphocytes was inhibited by prostaglandins and leukotrienes and was inversely related to the extent of pertussis toxinsensitive G protein activation. Reduced myoinositol incorporation into uraemic PHA-stimulated PBMNC may depend at least in part on their enhanced PGE2 and LTB4 release accompanied by increased intracellular cAMP production. In CRF impaired myoinositol incorporation into immune cells may prove the disarrangement in the early events of transmembrane signal transduction, which may share the responsibility for the cell-mediated immune defect in these patients.

Adult↗
PubMed1995

Expression of fibrinogen receptors and GPIIb molecules on uraemic platelets: effect of recombinant human erythropoietin therapy.

In 14 chronic uraemic patients on regular haemodialysis treatment mean numbers of fibrinogen (Fg) receptors and glycoprotein IIb (GPIIb) molecules on one blood platelet were 8370 +/- 1282 and 15,694 +/- 2102 respectively, and they were significantly lowered in comparison with those found in 14 healthy persons (37,826 +/- 966 and 57,994 +/- 6824 respectively). A high positive correlation was found between numbers of GPIIb molecules and those of Fg receptors both in chronic uraemic patients and healthy subjects. Four-month treatment of haemodialysis patients with recombinant human erythropoietin (rHuEpo) induced significant rises in haematocrit from 22.3 +/- 1.2 to 31.2 +/- 1.7%, blood platelet count from 164,000 +/- 27,000/microliters to 206,000 +/- 16,000/microliters and mean platelet volume from 7.08 +/- 0.29 fl to 8.26 +/- 0.22 fl. However, in haemodialysis patients numbers of the investigated surface platelet receptors, measured at 8 and 16 week of rHuEpo treatment, remained unchanged.

Adenosine Diphosphate↗
PubMed1994

[Some indices of peripheral blood oxidative metabolism during acetate and bicarbonate hemodialysis in patients with chronic uremia].

Whole blood superoxide anion generation, erythrocyte superoxide dismutase (SOD-1) and catalase activities and erythrocyte and plasma malonyldialdehyde concentrations were evaluated during cuprophane hemodialysis using bicarbonate and acetate dialysate. Superoxide anion generation by resting phagocytes was found to be significantly higher during bicarbonate hemodialysis, while its generation by opsonized zymosan--stimulated phagocytes did not differ between hemodialyses with both kinds of dialysates. Changes in the erythrocytes SOD-1 activities, found in the initial period of hemodialysis, were independent of the kind of dialysate, while erythrocyte catalase activity was higher during acetate hemodialysis in comparison with bicarbonate one. The kind of dialysate did not affect extent of erythrocyte membrane lipid peroxidation, while plasma lipid peroxidation was lower during acetate hemodialysis in comparison with bicarbonate one. In vitro studies with blood cells incubated with acetate or bicarbonate ions in concentrations, which are observed in vivo during hemodialysis, suggest that probably these ions do not directly affect superoxide anions generation, erythrocyte SOD-1 and catalase activities and erythrocyte membrane lipid peroxidation.

Acetates↗
PubMed1993

[Hepatocholangiocarcinoma].

In a 48-year-old man, using imaging methods, the presence was shown of a tumour in the right hepatic lobe. The serum AFP and DCP concentrations--the serological markers of hepatocellular cancer--were significantly increased, however, the histological examination of liver biopsy specimen demonstrated the structure of a cancer originating from the bile canaliculi. The material for study obtained during laparotomy and autopsy made possible the demonstration of the structure typical of both cholangiocellular carcinoma and hepatocellular carcinoma in separate foci. The mixed form of hepatocholangiocellular carcinoma is extremely rare and difficult for diagnosis which induced us to report own case.

Carcinoma, Hepatocellular↗
PubMed1992

T lymphocyte subsets and NK cell cytotoxicity in chronic hemodialysis patients. The effect of recombinant human erythropoietin (rHu-EPO) treatment.

We investigated subpopulations of T lymphocytes, NK cell number and cytotoxic activity in 14 chronic uremic patients on regular hemodialysis treatment. We observed a significantly decreased absolute lymphocyte number and percentage of CD3 cells. Relative numbers of CD16 cells were significantly elevated, but NK cell cytotoxic activity was within a normal range. Nine patients with chronic renal anemia on maintenance hemodialysis were enrolled in rHu-EPO treatment trial. The treatment was continued till the hematocrit level reached 30%. Each of the patients had corrected anemia and well-being. After 12 weeks of the treatment we observed in these patients decreases in CD3, CD4, CD8 and CD16 cell numbers and elevation of CD4/CD8 ratio. Cytotoxic activity of NK cells did not change significantly. Presented results indicate that chronic hemodialysis patients have significantly diminished lymphocyte number. rHu EPO treatment affects the T lymphocyte subsets inducing a deep decrease of CD8 and CD16 cell percentage leading to normalisation of the CD4/CD8 ratio.

Adolescent↗
PubMed1991

Modification of some lymphocyte functions in vitro by opioid receptor agonists and antagonist in chronic uremic patients and healthy subjects.

In ten chronic uremic patients on regular hemodialysis treatment in vitro experiments revealed that stimulation of opioid receptors with morphine did not significantly change the mitogen-induced proliferative response of peripheral blood lymphocytes and interleukin-2 (IL-2) receptor expression on PHA-stimulated lymphocytes, while it appreciably decreased surface transferrin (Trf) receptor expression on PHA-stimulated lymphocytes. However, metenkephalin inhibited mitogen-induced proliferation and surface Trf receptor expression on uremic lymphocytes without affecting IL-2 receptor expression on PHA-stimulated cells. In ten healthy subjects opioid receptor agonists did not significantly affect mitogen-induced proliferation of lymphocytes, except for the inhibitory effect of 10(-8) M morphine in relation to lymphocytes stimulated with an optimal pokeweed mitogen (PWM) concentration. At the same time, opioid receptor agonists depressed surface IL-2 and Trf receptor expression on PHA-stimulated normal lymphocytes. In most of our experiments naloxone itself, a non-selective competitive opioid receptor antagonist, decreased mitogen-induced lymphocyte proliferation and IL-2 and Trf receptor expression on PHA-stimulated lymphocytes. Moreover, most frequently naloxone did not reverse inhibitory effects of opioid receptor agonists on lymphocytes. The results seem to indicate that opioid receptor stimulation by high metenkephalin concentrations, which are observed in the uremic blood plasma, may share the responsibility for immunodeficiency in chronic uremic patients. Next, in the presence of opioid receptor agonists directions of changes in the mitogen-induced proliferative response may not follow the alterations of IL-2 and Trf receptor expression on both uremic and normal lymphocytes. Finally the results also suggest that naloxone may possibly exert effects which are independent of its action on opioid receptors on lymphocytes.

Adult↗
PubMed1991

Whole blood superoxide anion generation and efficiency of some erythrocyte antioxidant systems during recombinant human erythropoietin therapy of uremic anemia.

Six chronic uremic patients on regular hemodialysis treatment were given recombinant human erythropoietin (r-huEPO) in a dose of 50 U/kg of body weight intravenously thrice weekly for 14 weeks. Following r-huEPO therapy, unstimulated whole blood superoxide anion (O2) generation did not change significantly, while opsonized zymosan-stimulated whole blood O2 generation increased. At the same time, erythrocyte superoxide dismutase and, in particular, glutathione peroxidase activities were found to be reducing with concomitant lowering of erythrocyte malonyldialdehyde (MDA) concentrations and increase in plasma MDA concentrations.

Adolescent↗
PubMed1991

Free oxygen species metabolism during haemodialysis with different membranes.

In 10 chronic uraemic patients free oxygen species metabolism was evaluated during the early period of haemodialysis with cuprophane, polyacrylonitrile and polysulphone membranes. Cuprophane haemodialysis was found to induce an increased generation of superoxide anion by both unstimulated and opsonised-zymosan-stimulated peripheral blood phagocytes, which was accompanied by a significant depression of erythrocyte superoxide dismutase activity and augmented erythrocyte membrane lipid peroxidation. Haemodialysis with polyacrylonitrile membrane did not induce significant changes in superoxide anion generation, erythrocyte superoxide dismutase activity, and erythrocyte and plasma malonyldialdehyde concentrations. The same was found with polysulphone membrane, except for plasma malonyldialdehyde, the concentration of which was significantly decreased. Our results suggest that use of polyacrylonitrile or polysulphone membranes for haemodialysis seems to be beneficial with regard to free oxygen radical metabolism.

Adult↗
PubMed1991

[Generation of active oxygen compounds by whole blood granulocytes in patients with chronic renal failure].

In patients with chronic renal failure (crf) on conservative treatment whole blood granulocyte superoxide anion (O2-) generation was significantly decreased. Regular hemodialysis treatment did not induce its significant improvement. As granulocyte O2- generation determines production of other active oxygen compounds, this phenomenon may lead to the impairment of phagocyte bactericidal activity and increased susceptibility to infections in crf. Various methods of determination of active oxygen compounds give incomparable results.

Adolescent↗
PubMed1991

Treatment with thymic extract TFX for chronic active hepatitis B.

Eighteen patients with biopsy-proven chronic active hepatitis B (CAHB) and a significantly lowered CD4+/CD8+ cell number ratio were treated with thymic factor X (TFX): group I (n = 12) - for 12 months, group II (n = 6) for 6 months. As early as 14 days after starting the treatment a lowering of CD8+ cell numbers with a rise in CD4+/CD8+ cell number ratio were found. These changes continued to progress during the next months and were accompanied by decreased in NK cell numbers with enhancement of NK cell activity. Normalization of the biochemical and immunological parameters occurred after 5-6 months of the treatment. In both groups of patients seroconversion in the HBe system was observed after 9-12 months of the treatment. After two years complete clinical remission persists with normal biochemical and immunological findings and without reversion in the HBe system. The results seem to indicate that TFX has an immunostimulatory action and exerts beneficial effects on the course of CAHB.

Adolescent↗
PubMed1990

Superoxide anion generation and lipid peroxidation processes during hemodialysis with reused cuprophan dialyzers.

In 11 chronic uremic patients superoxide anion (O2-.) generation and lipid peroxidation processes were determined during hemodialyses with cuprophan dialyzers used three times. Intradialytic changes observed during the first 20 min of hemodialysis, that is the period of the most marked alterations, with consecutive uses of the same dialyzer included: decreased whole blood O2-. generation at rest and following stimulation with opsonized zymosan, decreased reductions in the erythrocyte superoxide dismutase (SOD-1) activity, increased reductions in the plasma malonyldialdehyde (MDA) concentrations, and unchanged erythrocyte MDA concentrations. However, immediately before hemodialysis with third-used dialyzer whole blood O2-. generation at rest and following opsonized-zymosan stimulation, and erythrocyte SOD-1 activity were slightly, but statistically significantly, lower (p less than 0.05), while plasma MDA concentrations were higher (p less than 0.05) than those before hemodialysis with first-used dialyzer; erythrocyte MDA concentrations remained unchanged. The results seem to indicate that dialyzer reuse may exert beneficial effects on whole blood O2-. generation and protect erythrocyte membrane lipids from peroxidation, but, however, it leads to slightly increasing predialysis plasma lipid peroxidation.

Adult↗
PubMed1989

[Effect of hemoperfusion on plasma oxidizing capacity and erythrocyte sensitivity to oxidation in patients with chronic uremia].

An effect of haemoperfusion on plasma oxidizing capacity and erythrocyte sensitivity to oxidation was investigated in patients with the chronic uremia. It was found that plasma oxidizing capacity measured with NTB reduction is more pronounced in patients with the chronic uremia than in normal subjects. Oxidizing capacity of plasma is increased at the beginning of haemoperfusion. This effect is clearly seen during single pass of blood through the column with activated carbon. Erythrocyte sensitivity to oxidation following their suspension in the normal saline with phosphate buffer and measured with ascorbic-cyanate test does not differ significantly in patients with the chronic uremia and healthy subjects and does not change markedly during haemoperfusion.

Adult↗
PubMed1989

Superoxide anion generation, erythrocytes superoxide dismutase activity, and lipid peroxidation during hemoperfusion and hemodialysis in chronic uremic patients.

In 11 chronic uremic patients superoxide anion generation in whole blood, both without and with opsonized zymosan stimulation, was lower than that in 11 healthy controls, while erythrocyte superoxide dismutase (SOD-1) activity and erythrocyte and plasma malonyldialdehyde (MDA) concentrations were elevated. During hemoperfusion (HP) and hemodialysis (HD) superoxide anion generation transiently significantly increased. Changes in the erythrocyte SOD-1 activity and plasma and erythrocyte MDA concentrations during HP suggested that this procedure exerted beneficial effects on lipid peroxidation. On the other hand, during HD erythrocyte membrane lipid peroxidation seemed to be enhanced even further; this phenomenon took place mainly within the dialyzer and a decrease in the erythrocyte SOD-1 activity seemed to be one of the contributing factors. Results of in vitro experiments with cross-incubation of erythrocytes and blood plasma and incubation of whole blood with cuprophan membrane suggest existence of an SOD-1 activator in the uremic blood plasma, which is possibly eliminated during HD.

Adult↗
PubMed1989

Effect of hemodialysis on T lymphocyte subsets, con-A-activated suppressor cell activity, and interleukin-2 receptor expression on lymphocytes in chronic uremic patients.

Effect of hemodialysis (HD) on some indices of immune response was studied in nine chronic uremics. Total lymphocyte, OKT4+, and OKT8+ cell numbers significantly decreased during the first 20 min of HD, and they were decreased till the third hour of the procedure, whereas the OKT4+/OKT8+ cell number ratio did not change significantly. Before HD, Con-A--activated suppressor cells exerted a stimulatory action on autologous responder cells measured in two-step culture. During HD, Con-A-activated suppressor cell activity transiently appeared, with its peak at 60 min after the start of HD. It was accompanied by a transient rise in lymphocyte count with spontaneous interleukin-2 (IL-2) receptor expression, whereas the number of cells expressing IL-2 receptor following phytohemaglutinin (PHA) stimulation was progressively decreased during HD. A significant correlation was found between the increment of Con-A-activated suppressor cell activity and the increment of spontaneous IL-2 receptor expression on lymphocytes during one single blood flow through the dialyzer. The results supply further evidence that HD may impose additional disturbances on immune regulation in chronic uremics.

Adult↗
PubMed1989

Hemodialysis and the acute-phase response in chronic uremic patients.

In comparison with healthy persons, chronic uremic patients on regular hemodialysis treatment had significantly higher blood serum concentrations of alpha 1-acid glycoprotein, ceruloplasmin and C4 complement component, while levels of haptoglobin, C3 and transferrin were lower. Serum alpha 2-macroglobulin and alpha 1-antitrypsin levels were similar in both groups. Hemodialysis with cuprophan membrane induced only slight changes in some of these glycoproteins during a 48-hour follow-up period. Seven hours after termination of hemodialysis slight, but significant, decreases in blood serum transferrin and alpha 1-antitrypsin concentrations were observed. Hemodialysis thus does not seem to induce a conspicuous acute-phase reaction.

Acute-Phase Proteins↗