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Biomedical subjects

M Lorenzo

Publications and source records attributed to M Lorenzo.

102 records · Page 6Linked to original sources
PubMed1982

[In vivo study of lipogenesis and glycogen synthesis in fetal tissues of the rat. Effect of dexamethasone].

Foetal rat liver lipogenesis in vivo decreased during the last two days of gestation. Whereas glycogen synthesis of the same tissue had increased between 20-21 days and sharply decreased during the last day. Treatment with dexamethasone increased glycogen synthesis and decreased lipid synthesis at the same time. Conversely, foetal rat lung lipogenesis increased and glycogen synthesis decreased during the last two days of gestation. Treatment with dexamethasone decreased both the rates of lipogenesis and the glycogen synthesis in the same observation period.

Animals↗
PubMed1981

Lipogenesis in vivo in maternal and foetal tissues during late gestation in the rat.

The rate of 3H2O incorporation into lipid in vivo progressively decreased in liver but increased in parametrial adipose tissue during the last 3 days of gestation. These changes seem to be related to those occurring in plasma insulin and progesterone concentrations during the same period. Foetal liver showed a high rate of lipogenesis, which sharply decreased before parturition. foetal lung lipogenesis increased during days 20 and 21 of gestation.

Adipose Tissue↗
PubMedDate not supplied

Possible association between Taenia solium cysticercosis and cancer: increased frequency of DNA damage in peripheral lymphocytes from neurocysticercosis patients.

Helminths, particularly some Schistosoma species, have been associated with cancer in humans. Neurocysticercosis, produced by cysticerci of the helminth Taenia solium, has been associated with the emergence of brain tumours and haematological malignancies. Local tumours, such as glioblastoma, could be explained by the induction of DNA damage in cells surrounding the cysticercus and chronically exposed to an inflammatory host response. However, systemic effects such as haematological malignancies are not easy to understand. The present work was conducted in Mexico to find out whether DNA damage arises in peripheral lymphocytes in patients with neurocysticercosis. We utilized a highly sensitive technique to analyse chromosomal aberrations, in-situ hybridization with probes against chromosomes 1, 2 and 4, and in addition the blocked-cytokinesis technique was used to determine the formation of micronuclei, a peculiar form of DNA damage. The study was made in lymphocytes from 8 patients before and after the administration of praziquantel, 1 of the 2 drugs used for neurocysticercosis treatment. The frequencies of chromosome aberrations and micronuclei in peripheral blood lymphocytes were higher in the infected patients as compared to those observed both in healthy donors and in the group of patients after praziquantel therapy. Our results suggest that chromosome aberrations induced in peripheral cells during neurocysticercosis could be associated with the development of haematological neoplasias.

Adult↗
PubMedDate not supplied

Hernia of foramen of Morgagni in adult: case report of laparoscopic repair.

The videolaparoscopic repair of a diaphragmatic hernia of Morgagni by external knot tying technique is described. A 69-year-old woman with subocclusive symptoms by intrathoracic migration of abdominal viscera had an immediate and complete postoperative recovery. The hernial sac was not excised. A four-year follow-up shows no hernia recurrence. This case indicated that the laparoscopic approach can be considered a suitable and safe procedure for treatment of Morgagni's hernia.

Adult↗
PubMedDate not supplied

[The production of a latex immunoglobulin conjugate for the diagnosis of Gardnerella vaginalis].

To obtain an anti-Gadnerella vaginalis latex globulin, specific immunosera to reference strain (ATCC 14018) and to a clinical isolation strain pool were produced. These immunosera were characterized using PAGE-SDS and immunoblotting where a close antigenic relation between the clinical isolation strains and the reference strain was observed. The latex globulin conjugates obtained from these 2 immunosera were evaluated in vitro with a resulting level of detection of 10 ufc/mL of Gardnerella vaginalis. These two conjugates were also evaluated in clinical samples and compared with the 6 vaginalis culture and the criteria considered for diagnosing bacterial vaginosis. The anti-strain pool (II) latex globulin conjugate turned out to be more sensitive and specific than anti-G. vaginalis ATCC strain (I) latex globulin.

Animals↗
PubMedDate not supplied

[Monitoring of hepatocyte xenotransplantation. Usefulness of various laboratory parameters].

The authors report a study in which they evaluate the efficacy of some laboratory parameters for monitoring intrasplenic hepatocyte xenotransplantation (mouse to rat) as an alternative to 99Tc-HIDA dynamic scan and histologic exam. Swiss mouse and wistar rat hepatocytes were obtained with collagenase digestion. Wistar male rats were used as recipient and were allocated into three groups: A) omotransplanted rats; B) xenotransplanted rats; C) xenotransplanted and immunosuppressed (Cyclosporin A: 20 mg/kg/daily orally) rats. All rats underwent > 70% hepatectomy. Blood samples were obtained daily from a femoral vein and AST, ALT, ALP, bilirubin, albumin and urea were measured. No statistical differences were observed among groups and the laboratory parameters tested can't be considered a valid technique to xenotransplant rejection monitoring.

Animals↗
PubMedDate not supplied

[Anhepatic rat, technic for reconstruction of venous circulation].

Authors report an experimental model of total hepatectomy in the rat. In this model whole liver was replaced by an autologous vascular prosthesis. On the contrary as reported in other experiences, in this model is maintained the cavo-caval flow, with absence of cavo-caval and porta-caval shunts. Anhepatic rats survival not exceeded two hours from surgery.

Animals↗
PubMedDate not supplied

[Hepatocyte transplantation. From experimentation to therapeutic attempts in man].

Recently, hepatocyte transplantation has widely increased its interest. This technique can be perspectively considered as an attractive alternative to orthotopic liver transplantation to support liver function in acute, chronic and genetic metabolic hepatic diseases. The authors review the most significant steps in the field of hepatocyte transplantation with particular reference to its clinical experimental application in genetic metabolic disorders and acute liver failure.

Animals↗