Biomedical subjects

M Lin

Publications and source records attributed to M Lin.

At least 91 records · Page 5Linked to original sources

PubMed2001

Biochemical and functional analysis of a conserved IGF-binding protein isolated from rainbow trout (Oncorhynchus mykiss) hepatoma cells.

Rainbow trout (Oncorhynchus mykiss) serum contains several IGF-binding proteins (IGFBPs) that specifically bind to IGFs. The structures of these fish IGFBPs have not been determined and their physiological functions are poorly defined. In this study, we identified a 30 kDa IGFBP present in rainbow trout serum and secreted by cultured trout hepatoma cells. This IGFBP binds to IGFs but not to insulin. This IGFBP was purified to homogeneity using a three-step procedure involving Phenyl-Sepharose chromatography, IGF-I affinity chromatography and reverse-phase HPLC. Affinity cross-linking studies indicated that this IGFBP binds to IGF-I with a higher affinity than to IGF-II. N-terminal sequence analysis of the trout IGFBP suggests that it shares high sequence identity with that of human IGFBP-1 in the N-terminal region. When added to cultured fish and human cells, the trout IGFBP inhibited IGF-I-stimulated DNA synthesis and cell proliferation in a concentration-dependent manner. The inhibitory effect of the fish IGFBP was comparable to those of human IGFBP-1 and -4. These results indicate that the IGFBP molecule is structurally and functionally conserved in evolutionarily ancient vertebrate species such as bony fish.

Amino Acid Sequence↗

PubMed2001

Australia's notifiable diseases status, 1999: annual report of the National Notifiable Diseases Surveillance System.

In 1999 there were 88,229 [corrected] notifications of communicable diseases in Australia reported to the National Notifiable Diseases Surveillance System (NNDSS). The number of notifications in 1999 was an increase of 3 per cent on notifications in 1998 (85,227) and the second largest reporting year since the NNDSS commenced in 1991. Notifications in 1999 consisted of 29,977 bloodborne infections (34% of total), 22,255 gastrointestinal infections (25%), 21,704 sexually transmitted infections (25%), 5,986 vector borne infections (7%),5,228 vaccine preventable infections (6%), 1,967 (2%) other bacterial infections (legionella, meningococcal, leprosy and tuberculosis), 1,012 zoonotic infections (1%) and 3 quarantinable infections (0.003%). Notifications of bloodborne viral diseases particularly hepatitis B and hepatitis C and some sexually transmitted infections such as gonorrhoea and chlamydia continue to increase in Australia. Steep declines in vaccine preventable diseases such as Haemophilus influenzae type b, measles, mumps and rubella continued in 1999. This report also summarises data on communicable diseases from other surveillance systems including the Laboratory Virology and Serology Surveillance Scheme (LabVISE) and sentinel general practitioner schemes. In addition this report comments on other important developments in communicable disease control in Australia in 1999.

Australia↗

PubMed2001

[Mutation and polymorphism in exon 4 of tuberous sclerosis complex gene].

OBJECTIVE: To study the characteristic of mutation and polymorphism in exon 4 of tuberous sclerosis complex gene(TSC1) in Chinese. METHODS: Twenty-five TSC patients and 23 parents from 21 families were enrolled. The mutation of exon 4 in these subjects was identified by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and further confirmed by direct sequencing. RESULTS: The normal controls had the same SSCP bands. In 25 TSC patients, a sporadic case was found to display variant banding pattern and be heterozygous for 352 insA mutation by sequencing. In 23 parents who were normal on clinical examination, another bandshift was found on a mother who had two affected children, which was confirmed as 347A-->C(Val42Val) single nucleotide polymorphism(SNP) by sequencing. CONCLUSION: The 352 insA mutation is a new causative mutation and the 347A-->C is a rare single nucleotide polymorphism.

Adolescent↗

PubMed2001

[Combination of mycophenolate mofetil with cyclosporine A and methotrexate as acute GVHD prophylaxis after unrelated donor allogeneic bone marrow transplantation].

OBJECTIVE: To evaluate the efficacy and safety of mycophenolate mofetil (MMF) in combination with cyclosporine A (CsA) and methotrexate (MTX) for prevention of acute graft versus host disease (GVHD) after unrelated donor allogeneic bone marrow transplantation (allo-BMT). METHOD: Twelve cases of unrelated donor allo-BMT were evaluated in a single center trial. The acute GVHD was prevented with 1 g MMF daily in addition to CsA 3 mg x kg(-1) x (-1) and MTX 10 - 15 mg at post BMT day1, day3, day6 and day11. RESULTS: Acute GVHD was found in one case (Grade IV) at the seventh day and two cases (Grade II) at the tenth day and seventeenth day after BMT. These patients were treated with a combination of MMF, methyprednisolone and CsA. The common adverse hematologic events of MMF was leukopenia. CONCLUSION: The preliminary study showed that MMF could be used effectively and safely for prevention of acute GVHD in unrelated donor allo-BMT.

Acute Disease↗

PubMed2001

[Involvement of mitochondrial membrane potential in the homoharringtonine induced apoptosis of leukemic T-cells].

OBJECTIVE: Investigation of the role of mitochondrial membrane potential (MMP) in the homoharringtonine (HHT)-induced apoptosis. METHODS: Annexin V staining, flow cytometry and confocal laser scan microscopy were used to observe the relationship between Bax, cytochrome C and MMP in the HHT-induced apoptosis of leukemic T lymphocytic line Molt-3. RESULTS: The induction of apoptosis by HHT resulted in the translocation of Bax from cytosol to mitochondrial membrane and the decrease of cellular MMP, followed by the release of cytochrome C from mitochondria to cytosol. CONCLUSIONS: Changes of mitochondrial membrane potential might play a critical role in the HHT-induced apoptosis of leukemic T-cells.

Apoptosis↗

PubMed2001

[Study on induction of leukemic cell apoptosis by antisense oligodeoxynucleotide of human telomerase RNA].

OBJECTIVE: To explore the antitumor activity of human telomerase RNA antisense oligodeoxynucleotide (As-ODN) to leukemic cells and its mechanisms. METHODS: As-ODN was transfected into HL-60 cells by liposomal transfection. Telomerase activity of HL-60 cells was examined by telomeric repeat amplification protocol (TRAP)-enzyme-linked immunosorbent assay (ELISA). Apoptosis was analyzed by morphology, DNA agarose gel electrophoresis and flow cytometry. RESULTS: The telomerase activity in HL-60 cells, being transfected with 0.1 - 2.0 micromol/L of As-ODN for 1 - 6 days, varied from 1.043 +/- 0.045 to 0.063 +/- 0.011 in a dose- and time-dependent manner. The proliferation of As-ODN-transfected HL-60 cells was lower than that of control, and the cells underwent apoptosis. The Ms-ODN (missense ODN)-transfected HL-60 cells didn't show these changes. CONCLUSION: As-ODN can inhibit the telomerase activity of HL-60 cells specifically and induce apoptosis.

Apoptosis↗

PubMed2001

[Aminopeptidase inhibitor Bestatin induces HL-60 cell apoptosis through activating caspase 3].

OBJECTIVE: To study the variation and significance of caspase 3 activity in the process of amino-peptidase inhibitor--bestatin (BS) inducing human leukemic cell apoptosis. METHODS: Cell apoptosis was evaluated by light microscopy, TUNEL labeling and flow cytometry (FCM). Caspase 3 activity was detected by colorimetry. The mitochondrial transmembrane potentials (DeltaPsi(m)) were detected by Rhodamine123 staining. RESULTS: The apoptotic morphology, apoptotic peak on FCM and positive Annexin V(FITC) on cell membrane showed that BS could induce HL-60 cell apoptosis in a dose- and time-dependent manner. Caspase 3 activity was significantly higher in the apoptotic cells than in control cells. The apoptosis induced by BS was inhibited by AC-DEVD-CHO. The DeltaPsi(m) of cells treated with BS declined. CONCLUSION: BS induces apoptosis of human acute leukemic cells through activation of caspase 3.

Aminopeptidases↗

PubMed2001

[Study on psychoprophylaxis and monoamines neurotransmitter of patients with burning mouth syndrome].

OBJECTIVE: Burning mouth syndrome (BMS) is a chronic ache disease, usually occurring in middle aged and old women. This study sought to understand the psychopathologic aspect and monoamines neurotransmitters in the plasma of the patients with BMS. METHODS: Thirty cases were selected (26 females, 4 males); 30 normal control subjects were similar to the BMS cases on age and sex. All subjects were required to complete the Eysenck personality questionnaire (EPQ), and the Self-report Symptom Inventory, Symptom Check List-90 (SCL-90) questionnaire. In case a subject's L (lie) score exceeded 50, she (he) would be removed from the test. 2 ml of blood was drawn from the subject under restine conditions with a fast in the morning to examine norepinephrine and epinephrine contents by high efficient liquid chromatography. Chi-square test, analysis of variance and t'-test were performed. RESULTS: The BMS group had higher scores of nervousness (N) and poikilergasia (P) and lower score of extro/introversion (E) as compared with the control (P < 0.05). The personality types in BMS group were focused on introversion and instability, but in the control group the types were focused on extroversion and stability (P < 0.05). The scores of 9 emotional factors of BMS group were significantly higher than those of the control group (P < 0.05), which indicated that the BMS patients had suffered from serial psychic disorders. The level of plasma norepinephrine in the BMS patients was higher than that of the control (P < 0.01). CONCLUSION: The personality of BMS patients raised body response to harmful stimulations, and obvious psychic disorders in the patient may cause the functional disorders in central and sympathetic nervous systems, which may be associated with BMS' occurrence.

Adult↗

PubMed2001

[Relationship between the symptom of xerostomia and non-stimulated salivary flow rates in patients with burning mouth syndrome].

OBJECTIVE: Many patients with burning mouth syndrome (BMS) complain of xerostomia, but the consistent relationship between the symptom of xerostomia and non-stimulated salivary flow rates remains unclear. This study tried to investigate the variety of non-stimulated salivary flow rates in patients with BMS. METHODS: The non-stimulated salivary flow rates of 52 BMS patients and 37 healthy controls were recorded. RESULTS: The non-stimulated salivary flow rates were low in the group of BMS patients, but there is no statistical difference when compared with that of the normal individuals. There was also no significant difference in non-stimulated salivary flow rates among theses three subtypes of BMS patients. Further, no significant difference of non-stimulated salivary flow rates was observed in patients with and without xerostomia. CONCLUSION: The non-stimulated salivary flow rate may be not associated with the symptom of xerostomia in patients with BMS.

Burning Mouth Syndrome↗

PubMed2001

[Phospholipase activities of Candida albicans isolates from oral pre-malignant lesions and oral cancers].

OBJECTIVE: The aim of this study is to compare activities of phospholipase of Candida albicans isolates from oral cavities of two groups of patients, including oral pre-malignant lesions and oral cancers. METHODS: A total of 55 isolates, including oral pre-malignant lesions (21), oral cancers (15), and normal health control (19), were studied using the precipitate zone assay on the egg yolk agar. RESULTS: No significant difference was found in phospholipase activities between these isolates from normal controls and oral pre-malignant lesions, while the activities of phospholipase of isolates from oral cancers were higher than those of the normal controls. CONCLUSION: Candida albicans may play different roles in different manners in these two closely related diseases.

Candida albicans↗

PubMed2001

[A study of millimeter wave's clinical and immunological effects on oral lichen planus patients].

OBJECTIVE: The aim of this study is to observe clinical effects of the millimeter wave on oral lichen planus (OLP) and its immune mechanisms. METHODS: 30 patients with OLP were randomized into the millimeter wave group and the control group. 15 patients in the millimeter wave group were treated with millimeter wave (7.1 mm, 43 GHz, 10 mW/cm2) radiation, while the other 15 patients in the control group were just given soothing treatment. Either before or after the therapy procedure, the clinical manifestations and the T-cell sub-grouping in the peripheral circulation of each patient were double-blindly evaluated. RESULTS: The pain-soothing effect in the patients treated with millimeter wave was much more obvious than that of the control group (P < 0.01). The peripheral blood CD8+ percent of millimeter wave group decreased greatly (P < 0.05), while no obvious CD8+ change was observed in the control group (P > 0.05). The millimeter wave group's CD4+/CD8+ improvement was also much more obvious than that of the control group (P < 0.05), and its CD4+/CD8+ ratio was completely recovered to the normal level. CONCLUSION: Millimeter wave can effectively relieve OLP patients' pain, and can regulate OLP patients' cellular immune condition. Millimeter wave might provide a new treatment method for OLP.

Adult↗

PubMed2001

[The examination of DNA repair capacity in the patients with oral precancerous lesion].

OBJECTIVE: The occurrence of oral precancerous lesion is related with not only carcinogenic environmental factors but also some self-regulating mechanisms. The genetic heredity factors determine individual's DNA repair capacity. The study is attempted to investigate the change of DNA repair capacity of the patients with the precancerous lesion. METHODS: The study is conducted in 16 pairs of oral precancerous lesion and normal controls by using host cell reactivation assay, while some dangerous factors such as smoking, alcohol intaking and family history of cancers are also recorded. RESULTS: The host cell reactivation in the patients with precancerous lesion is lower than that of normal controls (P < 0.05) and is shown to be an independent risk factor in the occurrence of oral precancerous lesion (OR: 3.25 95% CI = 1.01-10.49). CONCLUSION: The host cell reactivation is likely to be a helpful indicator for predict prognosis of cancer for the individuals with highly genetic sensitivity to cancer.

DNA Repair↗

PubMed2001

[The detection of phenol degrading strain in environment with specific primer of phenol hydroxylase gene].

A 684 bp oligonucleotide fragment was produced by PCR amplification from phenol-degrading strain Acinetobacter calcoaceticus PHEA-2 with the specific primers of gene encoding phenol hydroxylase. The nucleotide sequence of this fragment and its deduced amino acid sequence share 84% and 98% homology with the phenol hydroxylase gene and its deduced amino acid sequences of phenol-degrading strain Acinetobacter calcoaceticus NCIB8250. Sets of different aromatic compounds degrading strains were used to test this specific prime. The 684 bp-fragments were amplified only from phenol-degrading strains by PCR. When using this pair of primers to detect the bacterial isolates from wastewater discharged from coking plant, all the tested strains, which possess 684 bp characteristic fragment, showed the ability to degrade phenol in this study.

Acinetobacter calcoaceticus↗

PubMed2001

Phacoemulsification in patients with secondary glaucoma caused by uveitis.

PURPOSES: To evaluate the effect of phacoemulsification in the patients with uveitis, secondary glaucoma and complicated cataract. METHODS: Phacoemulsification and implantation of a foldable intraoucular len was performed in 12 patients(13 eyes) with uveitis, secondary glaucoma and complicated cataract. The complications, intraocular pressures (IOP), and visual acuity were observed postoperatively. RESULTS: No severe complication was found in the patients postoperatively or within the operation procedure. The visual acuity was improved after the operation (P < 0.05). The intraocular pressures and/or the number of antiglaucoma medications reduced in 3 months of the follow-up time. CONCLUSION: Phacoemulsification is the best way to treat the patients with secondary glaucoma and complicated cataract caused by uveitis.

Adult↗

PubMed2001

[Phacoemulsification in patients with cataract and overfiltering bleb after antiglaucoma surgery].

PURPOSE: To evaluate the effect of phacoemulsification in patients with cataract, overfiltering bleb, and low-tension after antiglaucoma surgery. METHODS: Phacoemulsification and implantation of a foldable intraocular len was performed in six patients(7 eyes) with overfiltering bleb, low-tension, and cataract. The complications, blebs, intraocular pressures(IOP), and visual acuity were observed postoperatively. RESULTS: No severe complication was found. The overfiltering blebs constricted and their walls became thicker three months after the operation. The visual acuity and IOP were improved (P < 0.05). CONCLUSION: Phacoemulsification may be another way to treat the patients with cataract and overfiltering bleb, low-tension after antiglaucoma surgery.

Aged↗

PubMed2001

[Influence of cell cycle in human trabecular cells induced by dexamethsone].

PURPOSE: To investigate the influence of dexamethesone (Dex) on cell cycle in human trabecular cells(HTC). METHODS: The trabecular specimens from human eyes were cultured in vitro. Then the cultured cells were immersed in DMEM solution containing 10(-7) M dexamethesone for three, five and seven days. The cell cycle was detected by Flow Cycometry. RESULTS: The G2 phase of cell cycle in HTC increased when HTC were treated by Dex. The G2 phase was 2% in control group. For three, five and seven days treated by Dex, the G2 phase were 8%, 16.3 and 19.4%. CONCLUSION: The influence of cell cycle might be induced by Dex, it may be related with the pathogenesis of glucocorticoid induced glaucoma.

Cell Cycle↗

PubMed2001

[The risk of sustained amenorrhea in patients with systemic lupus erythematosus receiving intermittent pulse cyclophosphamide therapy].

OBJECTIVE: To determine the risk of sustained amenorrhea in premenopausal women with systemic lupus erythematosus (SLE) receiving intermittent pulse cyclophosphamide (CTX) therapy. METHODS: Prospectively comparing the amenorrhea rate of 51 cases receiving intermittent pulse cyclophosphamide therapy versus that of 22 cases receiving intermittent pulse methylprednisolone (MP) therapy. RESULTS: The amenorrhea rate was higher in the CTX group (19.6%) than in the MP group (P = 0.025). In the CTX group, the amenorrhea rate of patients aged over 30 was higher than that of patients aged 30 or below 30 (P = 0.0018). CONCLUSION: Pulse CTX therapy in fertile women with SLE is associated with increased rate of sustained amenorrhea, and the older the patient is, the higher risk for sustained amenorrhea the patient runs.

Adult↗

PubMed2001

[Study on bolus cyclosphamide treatment for 64 cases of lupus nephritis].

OBJECTIVE: To report on the dosing, efficacy and side-effects of bolus cyclosphamide treatment for lupus nephritis (LN). METHODS: 64 consecutive cases of LN with 10 or more erythrocytes per high-power field, proteinuria (> 1 g of protein per day) and serum creatinine increased (> 133 mumol/L) were treated by bolus therapy with cyclophosphamide (CTX) given monthly for 6 months and then quarterly for 18 months. RESULTS: 49 patients had renal remission (defined as < 10 erythrocytes per high-power field, absence of cellular casts, excretion of < 1 g of protein per day and normal serum creatinine). The mean of doses was 1.1 g for each time (0.6-1.6 g), the mean of times of bolus CTX needed was 3.6 (1-8 times). The adverse events were amenorrhea (in 41% female patients), herpes zoster (in 13% patients) and hemorrhagic cystitis (in 1 patient). CONCLUSION: The results indicate that monthly bolus CTX therapy is effective and safe for patients with LN. Its adverse effect is relatively not a serious problem.

Amenorrhea↗