Search PubMed⌕ Search

Biomedical subjects

M Li

Publications and source records attributed to M Li.

At least 559 records · Page 31Linked to original sources
PubMed1999

[Preventing and treating effect of total oil of Microula sikkimensis (Hemsl.) on experimental hyperlipemia in rats].

OBJECTIVE: To study the effect of the total oil of Microula sikkimensis on an experimental hyperlipemia model of rat. METHOD: Using feeding method to set up the experimental hyperlipemia model and determining the biochemical criteria. RESULT: The total oil of Microula sikkimensis significantly decreased the contents of cholesterol, triglyceride and MDA in blood serum, and increased the rate between total cholesterol and high density lipoprotein. CONCLUSION: The study provides an important basis for exploiting and utilizing the total oil of Microula sikkimensis.

Animals↗
PubMed1999

[Author analysis of papers published in "Space Medicine & Medical Engineering" from 1988 to 1998].

Objective. To probe into the characteristics of authors, institutes and region distribution of papers published in "Space Medicine & Medical Engineering" (SMME), as well as its papers indexed and cited by CBMDisc, CB-ISITC and CSCD-CD98; to look into its core authors, core regions and core institutes. Method. Bibliometric method was used to analyse its published papers, cited papers and indexed papers. Result. 716 papers have been published in SMME, among which 622 were coauthor papers (86.9%); 94 were single author papers (13.1%); organization collaboration papers 109 (15.2%); institutes 548 (76.5%); colleges and Universities 132 (18.4%); hospitals 36 (5.1%). CBMDisc and CB-ISTIC index rate were 81% and 75% of its papers respectively, SMME's 39 papers were cited in 25 journals via CSCD-CD98, 10 of 42 core authors are cited. Conclusion. Papers published in SMME per issue have been increasing in 10 years, core region is Beijing. Core authors have been formed in SMME. Papers indexed by CBMDisc and CB-ISTIC are also increasing. But its citation status is not ideal, and high cited papers and high cited authors are few. Numbers and types of Natural Scientific Foundation supported papers are increasing.

Academies and Institutes↗
PubMed1999

[Cochleogram for assessing hair cells and efferent fibers in carboplatin-treated ear].

OBJECTIVE: This paper describes a convenient histochemical technique that allows one to simultaneously assess the cochlear efferent neurons and inner (IHCs) and outer hair cells (OHCs). METHOD: Selective labeling of the sensory cells and efferent fibers was carried out by first labeling the hair cells for dehydrogenase activity and then staining the efferent fibers for acetylcholinesterase activity. Double-labeled specimens were prepared as a surface preparation and counts were made of the number of hair cells and the number of discreate fiber bundles crossing the tunnel of Corti along the entire length of the cochlea. RESULT: The utility of this technique for assessing ototoxic damage was demonstrated by constructing cochleograms showing the percentage of missing hair cells and tunnel-crossing efferent fibers in chinchillas treated with carboplatin. In the region of the cochlea associated with most IHC loss and a part of OHC missing showed by succinate dehydrogenase in the region of hair cells; however, the loss of tunnel-crossing efferent fibers showed by acetylcholinesterase staining was closely correlated with the percentage of OHC loss. But, if only damage the IHC, the efferent fibers showed normal. CONCLUSION: These results suggest that carboplatin damaged efferent systems may be due to the damage of OHC.

Animals↗
PubMed1999

[Analysis of results of fundamental frequency and voice parameter in healthy young people].

OBJECTIVE: To study and analyzing the parameter values of voice in normal healthy young people. METHOD: In present paper, voice quality of 153 cases were collected and analyzed with Dr. Speech software. RESULT: Jitter was (0.18 +/- 0.07)%, Shimmer (1.60 +/- 0.74)%, HNR (Harmonics-to-Noise Ratio) (25.34 +/- 3.12) dB, SNR (Signal-to-Noise Ratio) (25.39 +/- 3.09) dB and NNE (Normalized Noise Energy(-16.95 +/- 3.57) dB. Average of fundamental frequency in male was (160.81 +/- 24.27) Hz, in female was (297.42 +/- 35.89) Hz and in all was (206.35 +/- 70.77) Hz. At the same time, the results of some correlative parameter about voice was taken. CONCLUSION: We considered that there were significant differences between gender about fundamental frequency in parameter values of voice. Three formant produced at 4.5 times, 8.64 times, 17.04 times of the fundamental frequency in male, at 2.07 times, 4.96 times, 7.28 times in female. This data was usefulness to evaluate voice results of normal or abnormal.

Adolescent↗
PubMed1999

[Determination of plasma homocysteine in normal adults by high performance liquid chromatography with fluorescence detection].

A rapid, isocratic high performance liquid chromatographic(HPLC) method with fluorescence detection was used for the determination of total homocysteine (Hcy) levels in human plasma. Prior to reversed-phase HPLC analysis, the plasma thiols were derivatized with ABDF(7-fluoroben30-2-oxa-1,3-diazole-4-sulfonamide), a thiol specific fluorogenic probe. A mobile phase consisted of 0.1 mol/L potassium dihydrogen phosphate (pH 2.1, containing 10% of acetonitrile) ensured base line separation of plasma Hcy. The mean recovery of this method was 97.75%. The intraday and interday coefficient of variations were 4.49% and 9.79%, respectively. The method is simple, sensitive, reproducible and very suitable for routine determination of plasma Hcy in human.

Adult↗
PubMed1999

[Study on the biologic function of extracorporeal bioartificial liver support system].

In this study, a total of 2 x 10(8) hepatocytes and nonparenchymal liver cells cultured as aggregate spheroids and a hollow fiber bioreactor were used to set up the extracorporeal bioartificial liver support system (EBLSS). Its function was determined throungh the analysis of some cells production. After 6 hours circulating of EBLSS, the total protein, albumin, alpha-fetoprotein and urea were markedly increased in the circulating media, and the DNA synthesis of rat hepatocytes cultured in the media was significantly increased (compared with control group, P < 0.01). This suggests that the EBLSS which consists of cultured human liver cells and hollow fiber bioreactor has the biologic function of liver and has a stimulative action on the proliferation of hepatocytes.

Cell Differentiation↗
PubMed1999

[Influence of EBV latent membrane protein 1 gene on the proliferation of epithelial cells].

In order to study the influence of EBV latent membrane protein (LMP1) gene on the proliferation of epithelial cells and the role of LMP1 creating to epithelial cell carcinomas, EBV LMP1 gene eukaryotic expression plasmid was transfected into human embryo kidney epithelial cells. The expression of LMP1 protein in transfected cells was detected. Observing the formation of colonies in soft agar, the value of MTT absorbed and the expression of PCNA, the results showed that the transfected cells grew vigorously and formed more colonies in soft agar, the MTT uptake and PCNA expression were apparently increased. The conclusion was that LMP1 gene could alter the biological behaviours of epithelial cells, promote cell growth, proliferation and transformation and endowed the transfected cells with growth characteristics like tumor cells.

Cell Division↗
PubMed1999

[Effects of IFN-gamma gene-modified hepatocytes on TGF-beta 1 and its receptor in mice infected with Schistosoma japonicum].

AIM: To explore the anti-schistosomal hepatic fibrosis effect and the changes in transforming growth factor-beta 1 (TGF-beta 1) and its receptors (TGF-beta RII) in S. japonicum infected mice after intrasplenic transplantation of gamma-interferon (IFN-gamma) gene-modified hepatocytes. METHODS: At 16 wk after infection with cercariae of Schistosoma japonicum, the mice were intrasplenically transplantated with murine hepatocytes which had been transfected with IFN-gamma gene-combinant adenovirus vector. ELISA, immunohistochemical and dot blot techniques were used to observe the dynamic changes in IFN-gamma, TGF-beta 1, TGF-beta RII and type I, III collagen. RESULTS: The intrasplenic transplantation of IFN-gamma gene modified hepatocytes effectively expressed IFN-gamma and obviously reduced the production and deposition of type I, III collagen as well as TGF-beta 1 and TGF-beta RII. CONCLUSION: IFN-gamma gene transplantation has anti-hepatic fibrosis efficacy in Schistosoma japonicum-infected mice, being related to its role of decreasing the expression of TGF-beta 1 and TGF-beta RII.

Animals↗
PubMed1999

[Finding of new FCC1/HN antigenic expressed sequence tag(ESTs) of Plasmodium falciparum].

AIM: To sequence the strong positive clones obtained by immuno-screening of Plasmodium falciparum FCC1/HN lambda gt11 cDNA expression library, and to elucidate the antigenic expressed sequence tags through sequencing the cDNA insert of these positive clones, and new antigenic ESTs could serve as a resource to pursue their corresponding antigen genes. METHODS: cDNA inserts of positive lambda gt11 phage clones were amplified by PCR. The PCR products, after purification, were cloned into the M13 mp18 sequencing vector. Single-stranded M13 DNA was prepared and sequenced. Then the acquired sequences were compared in homologies with EMBL/GenBank database on the PC/GENE software system and searched in NCBI (National Center for Biotechnology Information) GenBank using BLAST (Basic Local Alignment Search Tool) commond. RESULTS: Sequence C03 was part of the known P. falciparum antigenic heat shock protein 70 (Pfhsp70) gene, while the other 5 sequences were new P. falciparum antigenic expressed sequence tags (ESTs). CONCLUSION: The 5 new antigenic ESTs generated could serve as the breaking through points in our efforts to find out new P. falciparum antigen genes.

Animals↗
PubMed1999

[Expression of Schistosoma japonicun fatty acid binding protein gene in silkworm cells and larvae].

AIM: To express the fatty acid binding protein (Sj14FABP) gene of Schistosoma japonicun in the silkworm cells and larvae. METHODS: A 600 bp DNA fragment containing Sj14FABP gene was cloned into baculovirus transfer vector of pBacPAK His1 to construct recombinant transfer vector Sj14-pBac PAK His1. Coinfection was accomplished with this vector and Bombyx mori nuclear polyhedrosis virus (BmNPV) DNA in BmN cells. The recombinant virus of Bm-Sj14 was screened using dot-blotting. The BmN cells and silkworm larvae were infected with Bm-Sj14 to express Sj14FABF gene. Western blotting and ELISA were used to identify the antigenicity of the recombinant protein. RESULTS: Sj14FABP gene was successfully expressed in the BmN cells and silkworm larvae infected with Bm-Sj14. The product was a 18 kDa fusion protein. The yield in BmN cells was about 100 micrograms/1 x 10(6) cells and 33 micrograms/ml cell supernatant. In silkworm larvae, the product yield was 4 mg/ml haemolymph as well as 4.6 mg/g silkworm tissue. The recombinant protein could be recognized by Western blotting and ELISA using the sera from mice immunized with SWAP. CONCLUSION: Sj14FABP gene has been successfully expressed in BmNPV system and the product has high antigenicity.

Animals↗
PubMed1999

[Cloning and sequence analysis of histidine-rich protein-II gene fragment of Plasmodium falciparum yunnan strain].

AIM: To determine the nucleotide sequence of the partial gene of the histidine-rich protein II (HRP II) the Plasmodium falciparum PFD-3/YN and find out the differences of the HRP II gene sequence between this isolate and other isolates. METHODS: The histidine-rich protein-II gene fragment was amplified by polymerase chain reaction and cloned into M13 bacteriophage. M13-HRP II single strand DNAs of three positive clones were extracted, respectively. The nucleotide sequence of the HRP II gene fragment was determined by the dideoxy chain termination method. Using PCGENE software to compare and analyze the HRP II gene sequence among the different isolates. RESULTS: Different degrees of diversity of the HRP II gene sequences were found among Plasmodium falciparum PFD-3/YN(from China) and two other isolates (7G8 from Brazil and D10 from Gambia). The HRP II in the three isolates exhibited 70.3% homology in amino acid sequences and 68.6% homology in the nucleic acid sequences. CONCLUSION: There were differences in HRP II gene sequence among the Plasmodium falciparum PFD-3/YN and two other isolates (7G8 and D10).

Amino Acid Sequence↗
PubMed1999

[Sequencing and analysis of a cDNA clone CO111 of Plasmodium falciparum].

AIM: To sequence and analyse a cDNA clone CO111, reacting with immune sera obtained from rabbits immunized with Plasmodium falciparum and one McAb against P. falciparum. METHODS: cDNA clone was amplified by PCR. The PCR product was purified and polished with Klenow enzyme and ligated into the M13mp18 vector (digested by SamI) and then transformed into E. coli JM109. The positive recombinant was screened out by PCR and sequenced by the PRISM Dye Primer Sequencing Kit(ABI). The sequence was analyzed by the program from Geneva University and was compared by GenBank of EMBL. RESULTS: The nucleotide sequence of this cDNA clone contains an open-reading frame of 233 bp, which encodes a predicted polypeptide of 77 amino acid residues. The ratio between A + T and G + C is 3.16. The polypeptide is highly hydrophilic and flexible. Comparison among cDNA of P. falciparum from GenBank of EMBL showed that no sequence identical to this cDNA was found. CONCLUSION: A novel cDNA clone reacted with the antibodies against P. falciparum was isolated.

Amino Acid Sequence↗
PubMed1999

[Experimental studies of Chinese taihe chicken on effects of "Qi" reinforcing and "Yin" nourishing].

The "Qi" deficiency and "Yin" deficiency rats were fed with Chinese Taihe Chicken or common Chicken. The results showed: Chinese Taihe Chicken and common Chicken could all improve in part the "Qi" deficiency of rats caused by forced swimming, furthermore, the effect of Chinese Taihe Chicken was better than that of common Chicken. Chinese Taihe Chicken could obviously reduce the activity of adenosine triphosphatase of kidney tissue of "Yin" deficiency rats caused by injecting thyroxine. It suggested that Chinese Taihe Chicken could nourish the "Yin".

Adenosine Triphosphate↗
PubMed1999

[Extraction and purification of acidic mucopolysaccharide from Holothuria atra].

Mucopolyaccharide with molecular weight of 10253 Da was extracted and purified from fresh Holothuria atra Jaeger by means of enzymic and alkaline hydrolysis, potassium acetate and ethanol fractional precipitation. It was tested to be purified ingredient with agarose electrophoresis. The percentage content of galactosamine, glucuronic acid, fucose and sulfate in the mucopolysaccharide was 16.12%, 17.88%, 11.66% and 23.52% respectively.

Animals↗
PubMed1999

Clinical investigation of radiation retinopathy fundus and fluorescein angiographic features.

PURPOSE: To investigate the fundus and fluorescein angiographic features in the patients with radiation retinopathy. CLINICAL MATERIALS: Color fundus photography and/or fluorescein angiography from 13 patients with nasopharyngeal carcinomas received external beam radiation were retrospectively analyzed. RESULTS: In this study, 26 damaged eyes of 13 patients developed some degree of radiation retinopathy. The earliest and most common finding was macular microvascular changes (microaneurysms and/or telangiectasia), which was observed in 100% (26/26) of the eyes. Intraretinal hemorrhages, macular capillary nonperfusion, and mascular edema were noted in 84%, 50%, and 42% of the eyes, respectively. CONCLUSIONS: Radiation retinopathy is common after external beam radiation of nasopharyngeal carcinomas. The prominent changes include macular microvascular changes, intraretinal hemorrhages and macular capillary nonperfusion.

Adult↗
PubMed1999

[Calf trabecular cell culture in vitro, morphological and cytoskeletal ultrastructures and kinetics investigation].

OBJECTIVE: To establish the method of culturing calf trabecular cells (CTCs) in vitro, to understand the morphology and function of CTCs, to probe into the effect of resistance of aqueous outflow in the pathogenesis and mechanism about primary open angle glaucoma(POAG). To direct the clinical using of drugs and to open up new antiglaucomatous medicine by pharmacological studies of CTCs. METHODS: Trabecular meshwork was collected from twenty eyeballs of calf donors after slaughter. The tiusse was primarily cultured and cells were subcultured. The growing characteritics and morphological features of cultured primary and passaged cells were observed by light and electron microscopes. Cell kinetics of the third and tenth passaged cells were analysed using autoradiography and flow cytometry. The influence of the antiglaucomatous drugs 0.25 mg.ml-1 epinephrine (EPI) and 0.025 mg.ml-1 dipivalyl epinephrine (DPE) on cell kinetics of the third passaged cells was studied. RESULTS: The growing characteritics and morphological features of cultured CTCs were as same as those of human trabecular cells. Growing types of CTCs included most of epitheial cell and few of fibroblast. The amount of cellular microfilaments was reduced, DNA synthesis time(Ts) and cell cycle time(Tc) were obviously prolonged with passaged increasing. Antiglaucomatous drugs-EPI (0.25 mg.ml-1) and DPE (0.025 mg.ml-1) made microfilaments dissolving, Ts and Tc obviously prolonging. CONCLUSION: Establishing the method of culturing CTCs in vitro and understanding their morphology, function and pharmacological effects provided an important information for studying human trabecular cells and probing into the effect of resistance of aqueous outflow in the pathogenesis and mechanism about POAG. These studies indicated that antiglaucomatous drugs-EPI (0.25 mg.ml-1) and DPE (0.025 mg.ml-1) influenced obviously microfilaments and cell kinetics of the third passaged cells and suggested that it is not to be ignored that 1% EPI and 0.1% DPE may make CTCs' microfilaments dissolving and may inhibit CTCs' division and proliferation when they are used in clinical therapy.

Actin Cytoskeleton↗