Search PubMed⌕ Search

Biomedical subjects

M Levine

Publications and source records attributed to M Levine.

At least 505 records · Page 28Linked to original sources
PubMed1980

Persistence of herpes simplex virus genes in cells of neuronal origin.

The growth characteristics of the KOS strain of herpes simplex virus type 1 (HSV-1) in cell lines of nervous tissues origin were examined in an attempt to develop a tissue culture system mimicking the in vivo state of HSV-1 latency. We have previously reported that the B103 rat brain neuroma cell line is nonpermissive for growth of the KOS strain. In this report, we show that this nonpermissiveness is a temperature- and multiplicity-dependent phenomenon, with minimum virus yields at an elevated temperature and a low multiplicity of infection. Under these conditions, B103 cells survived infection with active wild-type or mutant HSV-1, whereas similarly treated Vero cells were killed. Six independent cultures of B103 cells surviving HSV-1 infection have been established. The surviving cells ceased production of any HSV-1 virus by 14 days postinfection and resumed growth and division at rates comparable to those of uninfected B103 cells. Survivor cells continued to express HSV-1-specific antigens, however, as detected by indirect immunofluorescence and by surface iodination followed by immunoprecipitation and polyacrylamide gel electrophoresis. The survivor cells did not express all of the surface proteins seen on productively infected B103 cells, and they were not susceptible to complement-mediated immune cytolysis with anti-HSV-1 antiserum. These results demonstrate that at least a portion of the HSV-1 genome is being harbored in these survivor cells.

Animals↗
PubMed1980

Mutant analysis of herpes simplex virus-induced cell surface antigens: resistance to complement-mediated immune cytolysis.

BHK-21 cells infected with temperature-sensitive mutants of herpes simplex virus type 1 strain KOS representing 16 complementation groups were tested for susceptibility to complement-mediated immune cytolysis at permissive (34 degrees C) and nonpermissive (39 degrees C) temperatures. Only cells infected by mutants in complementation group E were resistant to immune cytolysis in a temperature-sensitive manner compared with wild-type infections. The expression of group E mutant cell surface antigens during infections at 34 and 39 degrees C was characterized by a combination of cell surface radioiodination, specific immunoprecipitation, and gel electrophoretic analysis of immunoprecipitates. Resistance to immune lysis at 39 degrees C correlated with the absence of viral antigens exposed at the cell surface. Intrinsic radiolabeling of group E mutant infections with [14C]glucosamine revealed that normal glycoproteins were produced at 34 degrees C but none were synthesized at 39 degrees C. The effect of 2-deoxy-D-glucose on glycosylation of group E mutants at 39 degrees C suggested that the viral glycoprotein precursors were not synthesized. The complementation group E mutants failed to complement herpes simplex virus type 1 mutants isolated by other workers. These included the group B mutants of strain KOS, the temperature-sensitive group D mutants of strain 17, and the LB2 mutant of strain HFEM. These mutants should be considered members of herpes simplex virus type 1 complementation group 1.2, in keeping with the new herpes simplex virus type 1 nomenclature.

Animals↗
PubMed1980

The trial of a malpractice case: part two.

The following discussion was presented by the late Mr. Levine, one of the country's most successful malpractice lawyers, during a series of lectures on medical malpractice. It is an invaluable, no-holds-barred introduction for administrators, risk management directors, physicians and attorneys to the real world of malpractice litigation. Part One of this article appeared in the October 1979 issue of this journal.

Expert Testimony↗
PubMed1979

Combined modality therapy of hepatic metastasis. Northern California Oncology Group Pilot Study.

Twenty-two patients with adenocarcinoma metastatic to the liver were treated with rapid fractionation whole-liver irradiation (1350-2100 rads in 300-rad fractions) with simultaneous intrahepatic 5-fluorouracil (10-15 mg/kg/day) and intrahepatic Adriamycin 2.5-10 mg/m2/day) as part of a Phase I-II study. Of the 21 patients who completed therapy, 19 had colorectal carcinoma and 2 had metastatic adenocarcinoma of unknown origin. Objective response was judged by measurement of liver size, evaluation of liver function tests, and by liver scan or CAT scan of the liver. Ten of the 21 evaluable patients responded, yielding an overall response rate of 47.6%. The response rate in patients with colorectal carcinoma was 55% (10/19). At this time, median duration of response is 14+ weeks and median survival from onset of therapy is 15+ weeks. Hematologic and gastrointestinal toxicity were tolerable. No hepatic toxicity was documented. This combined modality therapy was found to be a safe effective method for the palliation of liver metastasis.

Adenocarcinoma↗
PubMed1979

Congress (and evaluators) ought to pay more attention to history.

In 1935, in 1965, and in 1972, the original Social Security Act and amendments to it were used by states to deinstitutionalize. In each instance a private, profit-making nursing home and board and care industry was stimulated. Poor care in such facilities was an unintended consequence of the legislation. Neither the Congress nor evaluators have clear concepts of what constitutes a life of decency and dignity for the chronically dependent. Behaviorally oriented social scientists and legislators have in common the difficulty of dealing with soft variables such as decency and dignity. In the absence of clear goals, care drifts below a lower threshold of acceptability and is corrected to some extent following public scandal. Social scientists need to learn to develop concepts and measurements suitable to monitoring the implementation of humane ideals.

Deinstitutionalization↗
PubMed1979

Fibrous proteins of normal and abnormal human epidermis.

Keratin proteins of human epidermis have been isolated from normal and abnormal tissues. The normal proteins whether removed from living layers (prekeratin) or from the stratum corneum (keratin) are shown to consist of three chains, molecular weights 65,000 (A), 60,000 (B) and 52,000 (C). When compared with the epidermal proteins isolated from abnormal tissues, differences can be detected in the electrophoretic pattern of the subunits. In general, relative amounts of the individual chains are altered and in specific cases or two of the polypeptides found normally are missing. All the alpha-fibrous proteins extracted are found to be very similar in amino acid composition.

Amino Acids↗
PubMed1979

The trial of a malpractice case: Part one.

The following discussion was presented by the late Mr. Levine, one of the country's most successful malpractice lawyers, during a series of lectures on medical malpractice. It is an invaluable, no-holds-barred introduction for administrators, risk management directors, physicians and attorneys to the real world of malpractice litigation.

Clinical Competence↗
PubMed1979

Evaluation of day treatment center effectiveness.

A study was undertaken to evaluate the efficacy of the Day Treatment Center as an alternative to rehospitalization for chronic psychiatric patients. The project utilized a subject as his own control design comparing equal time intervals before and during Day Treatment Center involvement. The study demonstrates the efficacy of the Day Treatment Center as a therapeutic modality that not only helps prevent hospitalization, but also assists in decreasing the average length of stay when admission is unavoidable.

Adult↗
PubMed1978

A comparison of the substrate specificities of cathepsin D and pseudorenin.

Cathepsin D, purified from hog spleen, releases angiotensin I from tetradecapeptide renin substrate and from protein renin substrates purified from hog and human plasma. However, the enzyme does not act on the naturally occurring renin substrate as it exists in plasma nor on purified substrate in the presence of plasma. Cathepsin D releases angiotensin I quantitatively from tetradecapeptide renin substrate and does not further degrade the angiotensin I on prolonged incubation. The pH optimum for cathepsin D prolonged incubation. The pH optimum for cathepsin D acting on tetradecapeptide renin substrate is 4.5, and there is very low activity above pH 7. These properties are very similar to those of pseudorenin, an angiotensin-forming enzyme originally isolated from human kidney, indicating that cathepsin D and pseudorenin may be identical.

Animals↗
PubMed1978

Structure of pyruvate kinase and similarities with other enzymes: possible implications for protein taxonomy and evolution.

The structure determination of pyruvate kinase shows that each subunit of the tetrameric molecule consists of three domains. The largest of these domains has a remarkable similarity to the structure of triosephosphate isomerase. Another domain shows similarities to many other nucleotide binding proteins. We discuss these similarities and their implications for current arguments on protein taxonomy and evolution.

Animals↗