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Biomedical subjects

M Leal

Publications and source records attributed to M Leal.

At least 145 records · Page 8Linked to original sources
PubMed1986

Rotative agitation RIA. A high sensitivity technique for hepatitis B surface antigen (HBsAg) determinations.

With the objective of finding a simpler and more sensitive method than conventional radioimmunoassay testing to detect HBsAg, we have modified the sample volume and first incubation procedure of the Ausria IIA method. There was an up to 9-fold increase in sensitivity without loss of specificity, in different assays, when the first incubation was carried out with a serum sample of 1 ml that was rotatively agitated for 22 h at room temperature. Additionally, 71 samples from which conventional testing had isolated hepatitis B core antibodies were tested with the modified rotative agitation technique and 9 of these specimens (12.67%) were positive for HBsAg. Routine employment of the rotative agitation radioimmunoassay technique to screen HBsAg by blood banks, to detect potential infectious donations that escape conventional testing, could be an effective as well as low-cost procedure to prevent post-transfusion hepatitis B.

Blood Banks↗
PubMed1985

Antibodies to human T-cell leukemia virus type III in hemophiliacs from Spain.

We tested serum samples from 50 hemophiliacs from Sevilla, Spain, for antibody to HTLV-III by indirect membrane immunofluorescence (IMI) and radioimmunoprecipitation with SDS polyacrylamide gel electrophoresis (RIP-SDS/PAGE). All had received commercial clotting factors from the United States with the exception of one hemophiliac who had never been transfused. Thirty-four (68%) reacted with HTLV-III-infected cells (H9/HTLV-III) by both methods, but not with the uninfected line (H9). Of 41 hemophilia-A patients tested, 28 (68%) were positive, and of nine hemophilia-B patients, six (66%) were positive. The nontransfused hemophilia-B patient was negative for antibody to HTLV-III by both methods. One patient with clinical AIDS tested positive as did six of seven with chronic unexplained lymphadenopathy. The eight individuals with AIDS or lymphadenopathy all had hemophilia A. We conclude that exposure to HTLV-III is widespread among asymptomatic hemophiliacs in Spain.

Adolescent↗