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Biomedical subjects

M L Collado

Publications and source records attributed to M L Collado.

8 recordsLinked to original sources
PubMed1993

Abnormal cervical cytology in pregnancy: a 17-year experience.

OBJECTIVE: To evaluate the safety and accuracy of colposcopy and colposcopically directed biopsy in pregnant women with abnormal cervical cytology. METHODS: A retrospective analysis of 612 gravidas with abnormal cervical cytology was conducted. Colposcopy and directed biopsy were performed using standard techniques. Two patients underwent diagnostic conization during the second trimester. One hundred twelve patients had procedures that provided a final specimen. Endocervical curettage was omitted. The transformation zone was fully visualized in all patients by the 20th week of gestation. Directed cervical biopsy was performed on the following patients: 1) with colposcopic evidence of invasion or cervical intraepithelial neoplasia (CIN) III, 2) with discordancy between colposcopy and cytology, 3) electing termination of pregnancy, and 4) whose anticipated reliability was even remotely questioned. RESULTS: A colposcopically directed biopsy was performed in 449 patients (73%). Ninety-one patients (15%) did not have biopsies because of normal colposcopic findings, and the remaining 72 patients (12%) had either CIN I or II. Thirty-nine of these patients (6%) were lost to follow-up. Colposcopically directed biopsy and colposcopic impression had a 95% concordancy within one degree of severity; however, 14% of CIN I colposcopic impressions and 54% of normal colposcopic findings turned out to be CIN III and CIN I or II, respectively. Ninety-five percent of the biopsy diagnoses correlated with the final pathology to within one degree of severity. CONCLUSION: The data confirm previous findings that colposcopically directed biopsy is a safe and reliable method of evaluating pregnant patients with abnormal cervical cytology.

Adolescent↗
PubMed1985

Effect of progesterone upon adenylate cyclase activity and cAMP levels on brain areas.

Changes induced by progesterone on adenylate cyclase activity and cAMP levels were determined in brain areas involved in the integration of sexual behavior in ovariectomized estradiol benzoate primed rats. Adenylate cyclase and cAMP concentrations were assayed in: preoptic area, ventromedial hypothalamus and cerebral cortex. Our results show a significant increase in both enzyme activity and cAMP levels at 2 and 4 hours after progesterone administration in all brain areas studied. These data suggest that the facilitatory effect of progesterone on sexual behavior involves an activating mechanism of this steroid upon the adenylate cyclase enzyme which results in an intraneuronal cAMP enhancement.

Adenylyl Cyclases↗
PubMed1985

Inhibition of human monocyte locomotion by products of axenically grown E. histolytica.

The supernatant fluid of axenically grown E. histolytica inhibits chemotaxis, chemokinesis and random mobility of human mononuclear phagocytes (MP) as measured in Boyden chambers. Human polymorphonuclear phagocytes (PMN) locomotion is apparently unaffected. The factor was found in comparable amounts in the supernatant fluid of axenic cultures of four E. histolytica strains that differed in their human pathogenicity and virulence, as well as in two entamoebas non-pathogenic for man. This dialysable and thermolabile MP-locomotion inhibiting entamoeba product (EP) can be absorbed out by incubation with MP, but not with lymphocytes, while partial absorption was observed using PMN. The MP-locomotion inhibitory effect of this EP was cancelled by inhibiting protein synthesis in the MP by means of cycloheximide. In vivo, this EP caused a delay in MP migration in Rebuck skin windows. The molecular weight of this EP lies between 478 and 765 by gel-sieve chromatography. Our results suggest a direct effect upon the cytoskeletal and locomotive apparatus of the MP. This MP-locomotion inhibiting EP could contribute to the paucity of the inflammatory reaction observed in the advanced stages of invasive amoebiasis and consequently also to the lack of scar tissue formation upon healing of amoebic lesions.

Adult↗
PubMed1980

Chemotaxis.

Chemotaxis is defined as the ability of living cells to determine the direction of their locomotion along a concentration gradient of attractant and repellant substances. A survey is given of the various in vitro techniques for detecting chemotaxis in leukocytes including monocyte macrophages, polymorphonuclear cells, lymphocytes and bacterial cells. Biological substances such as complement and its components, factors derived from lymphocytes and other proteins which cause chemotaxis are also described. Finally, reference is made to the relationship between chemotaxis and manifestations of disease (e.g. inflammatory response) and defects in the chemotaxis-inducting systems in man in certain diseases (Chediak-Higashi syndrome, lazy leukocyte syndrome, etc.). Possible means of therapy of abnormal chemotaxis in man are still in the discussion stage.

Antibody Formation↗
PubMed1980

Effect of rabbit spermatozoa on the incorporation of labeled precursors into endometrial macromolecules.

The presence of rabbit spermatozoa in the uterine horn of the doe over a 10-hr period induced an increase in the incorporation of leucine-14C and orotate-3H [from 230 +/- 50 to 520 +/- 32 and 146 +/- 41 to 432 +/- 40 (dpm 10(3) mg DNA; Mean +/- SEM)] into endometrial proteins and nucleic acids. The addition of actinomycin D or cyclohexamide significantly inhibited this process. The intraluminal administration of dibutyryl cyclic AMP (dbcAMP) (control group) induced an increase two times greater than that produced by spermatozoa. The increase in the incorporation of radioactive precursors into endometrial macromolecules induced by the spermatozoa is independent of the uterine carbonic anhydrase activation produced by this cell.

Animals↗