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Biomedical subjects

M Kunkel

Publications and source records attributed to M Kunkel.

82 records · Page 5Linked to original sources
PubMed1976

[Detection of H-antibodies in the diagnosis of listeriosis].

The technique of H-antibodies determination in serodiagnosis of listeriosis used at present is capable of detection not only H-antibodies but also flagellar somatic antibodies in the sera. An attempt was made to increase the specificity of H-agglutinins. An experience in the preparation of pure H-antigen is described. However, it is impossible to detect positive H-agglutination either with the aid of a mixture of flagellae and latex or with pure H-antigen. After determination of H-antigen by the classic Widal reaction 405 sera sent to Rostok Institute of Medical Microbiology and Epidemiology because of suspected listeriosis were subjected to absorption with O-somatic antigen of listeria; as a result of which in 85.9% of the sera the amount of H-agglutinins decreased by 1-3 dilutions. Whereas before the exhaustion 90 sera had the H-titre of 1:321 and higher, only 15 such sera remained after the exhaustion.

Adsorption↗
PubMedDate not supplied

Cryoprecipitates as a source of fibrinogen in treatment of disseminated introvascular coagulation (DIC).

Cryoprecipitates, in addition to containing factor VIII, contain about one third of the fibrinogen in the plasma from which they were derived. This fibrinogen is functional, as established by successfully preparing two congenital hypofibrinogenemics for major surgery by infusing cryoprecipitates. Cryoprecipitates and platelet concentrates are also used for patients with low levels caused by disseminated intravascular coagulation (DIC). We feel that these patients benefit not only from the factor VIII in the cryoprecipitates, but from the fibrinogen, which adds its support to the fibrinogen in platelet infusions, plasma, and whole blood. Such support makes it possible to heparinize such patients more heavily than would be safe without such preparation. The authors report three patients with severe and life-endangering DIC who were heavily heparinized, and supported with cryoprecipitates, as well as other blood fractions. Response to this therapy was excellent.

Adolescent↗
PubMedDate not supplied

Electrical and magnetic stimulation of the intercostal nerves: a comparative study.

The present study was performed in order to re-evaluate a previous report on motor nerve conduction measurement of the intercostal nerves (IN) by electrical stimulation (ES). In addition, it was aimed to compare ES with magnetic stimulation (MS). The 7th to 9th IN were investigated in 50 healthy volunteers, aged 20 to 80 years (mean age 35 +/- 16 years). The recording electrodes were placed over the cranial part of the rectus abdominal muscle. Stimulation was performed paravertebrally, and at a distance of 10 cm between stimulating and recording electrodes. The mean distal motor latency was slightly, but significantly longer after ES than MS (2.5 +/- 0.4 ms and 2.7 +/- 0.3 ms, respectively, p < 0.01). ES and MS resulted in similar mean motor nerve conduction velocities (NCV; 77 +/- 10 m/s and 79 +/- 10 m/s, respectively; p = 0.2). The amplitudes of the compound motor action potentials (CMAP) after distal stimulation versus proximal stimulation were significantly different in ES (2.5 vs. 1.9 mV; p < 0.01) and MS (3.6 vs. 1.6 mV; p < 0.001, resp.). In one subject, neither distal nor proximal ES evoked a CMAP. In eight subjects, proximal ES failed to elicit a CMAP. In all subjects, distal MS resulted in a visible CMAP, whereas in two subjects proximal MS was unsuccessful. Thus, MS proved to be significantly more effective in eliciting a CMAP (p < 0.05). Moreover, MS was agreed to be less unpleasant than ES. In summary, MS facilitates nerve conduction studies of the IN and is superior to ES regarding performance and acceptance.

Adult↗