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Biomedical subjects

M Kriz

Publications and source records attributed to M Kriz.

At least 19 recordsLinked to original sources

[Benign paroxysmal vertigo in children].

Based on our examination of 176 patients, we first selected, according to the general standards of diagnosing benign paroxysmal vertigo of childhood (children aged up to ten years, paroxysmal vertigo, ataxia) a group of 78 patients. Using multidisciplinary approach we have thus performed in these children the following examinations: an exhaustive anamnesis, laboratory examinations, clinical ORL (ENT), ophthalmological, neuropediatric examinations, x-ray of the cranium and the temporal bone, audiometric, vestibulogenic and EEG examinations. In the following stage we have, on the basis of the obtained data, selected 22 patients who met the restrictive standards for the diagnosis of a benign paroxysmal vertigo of childhood (typical clinical picture, isolated vestibular symptomatology, exclusion of the etiological factors, benign course of the disease). In our material the age range was between the second and the sixth year. After the sixth year of age we have not had any case of benign paroxysmal vertigo. We have not arrived at any significant fact which would indicate an etiological factor. There is no significant difference in the frequency of the disease between the two sexes, but we may assert that small girls slightly prevail. Normal psychomotoric development, normal intellectual faculties, absence of risk factors (only a twin had lower birth weight), absence of neurological abnormalities, regular EEG findings, pathological findings of the vestibular excitability together with absence of cochlear symptomatology, and evident clinical symptoms obviously indicate the right diagnosis. The evolution of the disease is exceptionally favorable.(ABSTRACT TRUNCATED AT 250 WORDS)

Child

Novel prolactin related mRNAs in rat pituitary cells.

From cytoplasm of rat pituitary GH4C1 tumour cells, anti prolactin anti-serum precipitates a polypeptide with apparent molecular weight of 75.000 in addition to prolactin. In vitro translation of size fractionated RNA shows that a 82.000 molecular weight PRL-like polypeptide is encoded by a mRNA larger than the 1 kb prolactin mRNA. Northern blot analysis shows that a rat prolactin cDNA probe hybridize to a 3.2 kb RNA and a 1.5 kb RNA in addition to the 1 kb PRL mRNA. The 82.000 molecular weight translation product and the 3.2 kb mRNA is also detected in rat anterior pituitary cytoplasm. We conclude that at least one high molecular weight mRNA which code for a prolactin-like polypeptide, is present in normal rat anterior pituitary gland and in GH4C1 cells.

Animals

Relationship between stimulated prolactin release from GH cells and cyclic AMP degradation and formation.

We have studied the relationship between the prolaction (PRL) release induced by thyroliberin (TRH) and theophylline and the formation and inactivation of adenosine 3', 5'-cyclic monophosphate (cyclic AMP) in cultured rat-pituitary cells (GH3 cells). TRH, which stimulated prolactin release, increased cyclic AMP formation and stimulated transiently both the low- and high-Km cyclic phosphodiesterases. The maximal effect on the phosphodiesterase was observed at 30 mM TRH. The stimulatory effect of TRH on the activity of the cyclic AMP phosphodiesterases was duplicated by incubation of the cells with cyclic AMP (2-10 mM). In washed particulate GH3 cell fractions, TRH increased the adenylyl cyclase activity up to 180%. Treatment of GH3 cells with theophylline stimulated the release of PRL and inhibited cyclic AMP degradation probably leading to the measured increase in cellular concentrations of the nucleotide. The effects of TRH and theophylline on cellular cyclic AMP concentrations and on PRL release were additive. There was a positive correlation between PRL release and cellular cyclic AMP concentration (r = 0.97). The elevations observed in cellular cyclic AMP concentration after TRH treatment are due to increased formation which in turn leads to phosphodiesterase activation. Therefore, cyclic AMP formation appears to be an intermediary step in the stimulus-secretion coupling caused by the tripeptide.

3',5'-Cyclic-AMP Phosphodiesterases

Hypoxia-induced pulmonary vasoconstriction: effects of fentanyl following different routes of administration.

Recent investigations have revealed that intravenous anesthetics, including fentanyl, do not reduce the pulmonary vasoconstrictor response to alveolar hypoxia. In contrast, the response is markedly reduced or abolished by inhalation anesthetics. Recent investigations have demonstrated that the route of administration is of importance. Halothane, which inhibits the response when administered via the airways, behaves more like an intravenous anesthetic following administration via the blood stream, provided the alveolar concentration has been kept low (Bjertnaes et al. 1977). It was therefore a distinct possibility that the lack of any damping effect of fentanyl on the response could be due to the route of administration rather than to a different pharmacological property. We have tested this hypothesis by introducing fentanyl in nebulized form via the airways in one group of isolated rat lungs, and via the blood stream in another group. We found, however, no effect of fentanyl on the pulmonary vasoconstrictor response to hypoxia, regardless of the route of administration. Plasma concentrations of fentanyl were determined by radioimmunoassay and compared with those encountered in anesthetic practice.

Anesthesia, Inhalation

[Prolactin].

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Female

[Epidural analgesia--effect on the fetus and uterine activity].

The effect of continuous epidural analgesia on the fetus, newborn, and uterine activity was studied in a group of 50 parturients. No untoward effects, either in the fetus or uterine activity, that could be ascribed to this procedure, were observed. The authors use their own modicifation of the epidural block by titrating the amount of the local anesthetic according to the intensity of pain in labour.

Anesthesia, Epidural