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Biomedical subjects

M Kozuru

Publications and source records attributed to M Kozuru.

At least 73 records · Page 4Linked to original sources
PubMed1977

A case of lambda type tetramer Bence-Jones proteinemia.

A 50-year-old woman was found to have an extramedullary plasmacytoma of the thoracic vertebrae and a bone marrow plasmacytosis of 72%. In the serum of this myeloma patient, M-bow percipitin line was seen only with anti-lambda chain serum, but not with antisera against mu, alpha, gamma, delta, ipsilon and kappa chains. Gel filtration of the serum on the Sephadex G-200 column demonstrated the monoclonal lambda chain in the A-fraction along with transferrin and suggests a molecular weight of 88,000 and a tetrameric light chain. Bence-Jones protein has never been detected in the patient's urine.

Bence Jones Protein↗
PubMed1976

Specific precipitating antibody to human urinary erythropoietin.

In the present experiments, human urinary erythropoietin was purified from the urine of a patient suffering from pure red cell aplasia, using ethanol precipitation, gel filtration on the Sephadex G-100 column and electrofocusing. The anti-human urinary erythropoietin was prepared by injecting the isolated erythropoietin with Freund's complete adjuvant into rabbits intracutaneously. The specificity of the anti-human urinary erythropoietin was ascertained by a double diffusion technique and immunoelectrophoresis, observing a single precipitation line at the alpha2-globulin region against human serum or human urinary erythropoietin (Standard B). This precipitating anti-human urinary erythropoietin inhibited completely the effects of erythropoietin (Standard B) on the heme synthesis of bone marrow cells in vitro. The precipitating antigen with the specific anti-human urinary erythropoietin, that is, erythropoietic stimulating factor of the fraction 2, had the ability to enhance heme synthesis in bone marrow cells in vitro. The specific anti-human urinary erythropoietin makes it possible to determine the serum erythropoietin levels in various diseases by means of quantitative immunoelectrophoresis.

Animals↗