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Biomedical subjects

M Kosuge

Publications and source records attributed to M Kosuge.

52 records · Page 3Linked to original sources
PubMed1994

Isolation of intact transition protein 4 from boar late spermatid nuclei.

Boar transition protein 4 was extracted with acid from the late spermatid nuclei, and separated from the transition protein-degrading proteases by ion-exchange chromatography on Fractogel EMD SO3- 650 (M). The transition protein was further purified by HPLCs on Nucleosil 300 7C18 and Diol-200. The circular dichroic spectra of the protein with and without dithiothreitol showed that the protein had beta-form predominantly. Although sodium dodecyl sulfate affected the tertially structure of the protein, the beta-form was well retained. These indicate that the protein has a structure-forming potential for the beta-structure.

Animals↗
PubMed1994

Isolation of intact transition protein 1 and 3 from boar late spermatid nuclei.

Boar transition protein 1 and 3 were extracted with acid from the late spermatid nuclei, separated from the TP-degrading proteases by ion-exchange chromatography on Fractogel EMD SO3- 650 (M), and further purified by HPLCs on Diol-120 and on Hitachi #3057, respectively. The circular dichroic spectra of the transition proteins with and without dithiothreitol showed that they have beta-form predominantly. Although sodium dodecyl sulfate partially induced helical structure, the beta-form was considerably retained. These indicate that the transition proteins have a structure-forming potential for the beta-structure.

Animals↗
PubMed1993

Closure of ventricular septal rupture caused by acute myocardial infarction using a transaortic balloon catheter.

We report a case of successful transaortic closure of a postinfarction ventricular septal rupture using a Swan-Ganz catheter. The method markedly reduced a left-to-right shunt and the improved hemodynamic condition of the patient, an 81-year-old woman, was maintained for about a week before surgical treatment. Inflation of the balloon with water made the balloon visible via echocardiography and was effective in maintaining inflation and echocardiographic follow-up.

Aged↗
PubMed1984

A procedure to elongate the stomach tube in the esophageal replacement.

When the stomach tube has to be lengthened in case of antethoracic esophageal replacement, a circumferential seromuscular incision is made and the lesser curvature side of the tube is cut through, in the same line of incision. The rent produced is longitudinally suture-closed, by which a definite elongation (about 2 cm by each incision) is obtained. In our department the antethoracic use of the stomach tube has been carried out since 1968, the elongation procedure was started in 1973 as a trial, then its application became more frequent since 1978 and was performed whenever the tube seemed to be deficient in length. The stomach tube was used in 40 cases, of which 17 underwent the elongation procedure. With regard to the suture leak at the site of the antethoracic esophagogastric anastomosis, the incidence was 50 per cent from 1968 to 1972, 54 per cent from 1973 to 1977 and 22 per cent in the last 5 years. Thus, a distinct improvement has occurred with time.

Esophagus↗
PubMed1978

Computer simulation of Gumboro disease outbreak. II. Results obtained with models G-1 and G-2.

The authors conducted a computer simulation with their Models G-1 and G-2 for Gumboro disease ten times in each of the following initial conditions: (1) size of population, 50, 100, and 1,000 chickens; (2) age of housing, 1, 7, 14, and 21 days; (3) nine levels of parentally conferred immunity in one-day-old chicks; (4) four levels of virus contamination; and (5) three steps of coefficient for aggravating morbid status. Every simulation was operated up to the age when all the birds of a flock turned to be insusceptible so as to yield the daily numbers of chickens (1) susceptible, (2) diseased, (3) immunized, and (4) removed, and (5) the accumulation of diseased chickens. The innate resistance, parentally conferred immunity, virus contamination, and morbid status were expressed in such values that they could be compared with one another. As a result, Model G-2 produced a more realistic epizootic pattern than Model G-1, but both models concealed the effect of differences in size of population and in age of housing. Notwithstanding the incompleteness of the models, the computer simulation gave valuable information for a further advancement in this series of studies.

Animals↗
PubMed1977

Prototype of simulation models for epizootics in domestic animals.

Based on the Reed-Frost model (Model I), the authors conducted computer simulation of an epizootic model (Model II) constructed on the assumption that any infected animal in a group, after a given time-period of infectivity, would be removed from the group at the beginning of the next time-period. Models I and II were simulated 100 times for each of the different conditions, viz. the initial size of group, 100 and 1,000, the five steps of contact rate or contact size, and the five more steps of contact rate for the group of 1,000 animals in Model I. From the results obtained, it is believed that as a constant parameter, contact size may be preferably used instead of contact rate in these models. Model II mostly gave higher morbidities than Model I, and earlier termination of epizootics, except the simulation with the smallest contact size. This fact may be due to the effect of herd immunity involved only in Model I. The long duration of epizootic was demonstrated in two of the 100 simulations of Model II with 1,000 individuals and contact size 1. This is characteristic of probabilistic models which are really instructive to studying the flow of epizootic.

Animals↗