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Biomedical subjects

M Kosaka

Publications and source records attributed to M Kosaka.

At least 181 records · Page 10Linked to original sources
PubMed1982

Separate detection of glucose-6-phosphate dehydrogenase from 6-phosphogluconate dehydrogenase by DEAE-paper chromatography.

Red blood cell lysates were applied to DEAE cellulose paper for detection of glucose-6-phosphate dehydrogenase. Glucose-6-phosphate dehydrogenase (G-6-PD) was separated from 6-phosphogluconate dehydrogenase (6-PGD) by DEAE-cellulose paper-chromatography and their activities were detected on the chromatogram by applying the reaction mixture. The fluorescence of NADPH formed by G-6-PD was not interfered with by 6-PGD activity. This is a sensitive procedure which detects slight loss of G-6-PD in red blood cells and could be applied to the detection of G-6-PD deficiency.

Chromatography, DEAE-Cellulose↗
PubMed1980

Relationships between hypothalamic catecholamines, blood pressure and body temperature in spontaneously hypertensive rats.

Rectal temperature, catecholamine contents in the hypothalamus, and blood pressure were measured in spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY). SHR had a significantly higher rectal temperature than WKY. Noradrenaline contents were lower in SHR than WKY. Noradrenaline was inversely correlated with the blood pressure and body temperature in SHR. In subgroups of SHR, differences in values of the parameters mentioned above were also observed.

Animals↗
PubMed1976

Serial cerebral angiography in stereoscopic magnification.

A ceiling x-ray tube support with synchronous up and down movements is described for obtaining two parallel x-ray beams with a short focal-film distance. Stereoscopic cerebral angiography is performed with direct serial magnification using a heavy-duty anode tube with a 0.1 mm focus. This technique combines the advantages of magnification and stereoscopic angiography. Small arterial and venous changes can be observed clearly in three dimensions. The usefulness and limitations of this method are discussed using clinical and experimental data.

Adult↗