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Biomedical subjects

M Kobari

Publications and source records attributed to M Kobari.

At least 145 records · Page 8Linked to original sources
PubMed1985

Prognosis of the pancreatic cancer in terms of the regional lymph node reaction.

In order to elucidate the immunological defense mechanism in tumor bearing hosts against pancreatic cancer, tissue reactions of the regional lymph nodes were investigated in terms of extension and prognosis of pancreatic cancer. As the site for investigation of lymph nodes, the paracortical area, germinal center and sinus histiocyte systems were selected. In each case paracortical hyperplasia, germinal center hyperplasia and sinus histiocytosis were graded and the relationship between grade and prognosis of resected pancreatic cancer was studied with the following results. The grades of each tissue reaction in cases without lymph node metastasis were higher than that observed in cases with metastasis. There was a significant difference in sinus histiocytosis (p less than 0.01). Higher grade tissue reactions were observed in cases with primary lesion histologic type classified as papillary adenocarcinoma or as well-differentiated tubular adenocarcinoma as compared to those in cases of poorly differentiated histologic type. In pancreatic cancer the prognosis was better in cases with higher grade of tissue reactions of the regional lymph nodes. In cases with pancreatic cancer both cellular immunity and humoral immunity are involved in the host defense mechanism. It was considered that sinus histiocytosis is significant as an indicator for good prognosis and a factor to inhibit progression in pancreatic cancer.

Adenocarcinoma↗
PubMed1985

[Prognosis of the pancreatic carcinoma in terms of the regional lymph node reaction].

In order to elucidate immunological defense mechanism in tumor bearing host against pancreatic carcinoma, tissue reactions of the regional lymph nodes were investigated in terms of extension and prognosis of pancreatic carcinoma. As the site for investigation of lymph node reaction paracortical area, germinal center and sinus histiocytosis system were selected. In each case paracortical hyperplasia, germinal center hyperplasia and sinus histiocytosis were graded and the relation between such grade and prognosis of resected pancreatic carcinoma was studied with following results. The grades of each tissue reaction in cases without lymphatic node metastasis were higher than that observed in cases with metastasis. There was significant difference in sinus histiocytosis (p less than 0.01). Higher graded tissue reactions were observed in cases with primary lesion with histologic type classified as papillary adenocarcinoma or as well differentiated tubular adenocarcinoma as compared to those in cases of poorly differentiated histologic type. In cases with pancreatic carcinoma the prognosis was better in cases with higher grade of tissue reactions of the regional lymph nodes. In cases with pancreatic carcinoma both cellular immunity and humoral immunity are involved in host defense mechanism. It was considered that sinus histiocytosis is significant as an indicator for good prognosis and a factor to inhibit progression in pancreatic carcinoma.

Adenocarcinoma↗
PubMed1985

[Hematogenous metastasis in pancreatic cancer].

Out of 248 pancreatic cancer patients, 82 with hematogenous metastases were clinicopathologically analyzed. The incidence of hematogenous metastases on laparotomy were 31% in the liver, 1.2% in the lung, and 0.4% in the adrenal gland and navel. The incidence of liver metastasis, which was present in 23% and 46% of the patients with carcinoma of the head of the pancreas and of the body and tail of the pancreas, respectively, was higher than that of carcinomas of the other digestive organs. In the autopsy findings of the cases submitted to tumor resection, early metastases to the liver, lung, cerebellum and ovary were recognized to have occurred postoperatively. A higher rate of liver metastasis with lymph node involvement in the early stage of the disease was peculiar to pancreatic cancer.

Adult↗
PubMed1984

Blood flow velocity in the pial arteries of cats, with particular reference to the vessel diameter.

The blood flow velocity and diameter of feline pial arteries, ranging in diameter from 20 to 200 microns, were measured simultaneously using a newly developed video camera method under steady-state conditions for all other parameters. There was a linear relationship between blood flow velocity and pial artery diameter (y = 0.340x + 0.309), the correlation coefficient being 0.785 (p less than 0.001). The average values for blood flow velocity in pial arteries less than 50 microns, greater than or equal to 50 but less than 100 microns, greater than or equal to 100 but less than 150 microns, and greater than or equal to 150 microns in diameter were 12.9 +/- 1.3, 24.6 +/- 3.4, 42.1 +/- 4.7, and 59.9 +/- 5.3 mm/s, respectively. Blood flow rate was calculated as a product of the cross-sectional area and the flow velocity. The blood flow rate increased exponentially as the pial artery diameter increased (y = 2.71 X 10(-4) x2.98). The average values for blood flow rate in pial arteries less than 50 microns, greater than or equal to 50 but less than 100 microns, greater than or equal to 100 but less than 150 microns and greater than or equal to 150 microns in diameter were 12.8 +/- 1.5, 122.1 +/- 24.8, 510.2 +/- 74.8, and 1524.2 +/- 174.4 10(-3) mm3/s, respectively. Hemorheological parameters such as the wall shear rate and Reynolds' number were also calculated. The data obtained provide a useful basis for further investigations in the field of cerebral circulation.

Animals↗
PubMed1984

Establishment of a human pancreatic cancer cell line and detection of pancreatic cancer associated antigen.

We succeeded in an establishment of a human pancreatic cancer cell line (PK-1) from liver metastasis of pancreatic cancer. Primary pancreatic cancer cells grew as islands surrounded by fibroblastic cells. However, these fibroblastic cells were gradually omitted by the polygonal shaped cancer cells. This cell line contained neither zymogen granules nor trypsin indicating that this pancreatic cancer originated from pancreatic duct cells. Modal chromosome numbers of this cell line were 42 and 72 and the doubling time was 48 hr. This cell line was transplantable in athymic nude mice to form progressive tumors which had histology similar to that of the original cancer (papillotubular adenocarcinoma). Neither AFP nor ferritin but CEA was detected on the surface and in the cytoplasm of this cell line in indirect immunofluorescence. Rabbit antiserum against this pancreatic cancer cell line detected pancreatic cancer associated antigen besides CEA in the culture supernatant. This antiserum reacted with sera from patients with pancreatic cancer to form a distinct precipitin line in agarose gel which fused with the precipitin line formed between the culture supernatant of this cell line and the antiserum.

Animals↗
PubMed1984

Effects of immunosuppressive acidic protein on DMBA-induced pancreatic cancer in rats.

In order to determine the effect of immunosuppressive acidic protein (IAP) on the formation of pancreatic carcinoma, rats of the Sprague-Dawley strain were embedded with 7,12-dimethyl-benzanthracene (DMBA) in the pancreas with and without administration of IAP. In these animals, the growth of pancreatic cancer was studied both immunologically and histologically. Tumor was induced in 51 animals (85%) of 60 treated with embedding of 1 mg DMBA alone. Tumors began to appear from the 16th week after the embedding. Among animals in which tumor was induced, tubular adenocarcinoma and pleomorphic carcinoma accounted for 55% of the cases. When administration of IAP was combined, the period required for development of tumor was shortened. It became shorter with increases in the dosage and frequency of administration of IAP. In animals which received IAP in a mean dose of 75 mg/kg the area showing cancerous changes appeared as early as at the 8th week after the embedding of DMBA. A significant increase in the volume of tumors was seen in the group treated with IAP as compared to the group not treated with IAP. Animals which received IAP in increasing doses and frequencies showed an accelerated increase in the volume of the tumors which underwent cancerous changes. IAP was eliminated from the serum of rats within 72 hr after the administration, and acid protein was clearly recovered from the serum when tumors proliferated. These findings indicate that the acceleration of carcinogenesis in DMBA-induced pancreatic carcinoma may be attributable to the immunosuppressive effect of IAP administered and tend to be dependent on the dosage and frequency of its administration in the early phase of tumor induction.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1984

[Effects of immunosuppressive acidic protein on 7, 12-dimethylbenzanthracene (DMBA) induced pancreatic cancer in rats].

In order to determine the effect of immunosuppressive acidic protein (IAP) on the formation of pancreatic carcinoma, rats of Sprague-Dawley strain were embedded with 7, 12-dimethylbenzanthracene (DMBA) in the pancreas with and without administration of IAP as a promotor, which was purified from the ascites obtained from cancer bearing patients, to induce pancreatic carcinoma experimentally. In these animals, growth of pancreatic cancer was studied both immunologically and histologically. Out of 60 rats treated with embedding of 1 mg DMBA alone, tumor was induced in 51 animals (85%). Tumors began to appear from the 16th week after the embedding. Among animals in which tumor was induced, tubular adenocarcinoma and pleomorphic carcinoma accounted for 55% of the cases. When administration of IAP was combined, the period required for development of tumor was shortened. It became shorter with an increase in the dosage and frequency of administration of IAP. In animals received IAP at a mean dosage of 75 mg/kg the area showing cancerous changes appeared as early as at 8th week after the embedding of DMBA. The acceleration of carcinogenesis in DMBA induced pancreatic carcinoma may be attributable to the immunosuppressive effect of IAP administered and tend to be dependent on the dosage and frequency of its administration in the early phase of tumor induction.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1983

Transfer function through regional cerebral cortex evaluated by a photoelectric method.

A new technique is reported for obtaining the transfer function through the regional cerebral cortex. Tissue carbon-black (CB) dilution curves were recorded with a photoelectric apparatus used as a densitometer head in cat cortex in situ following intracarotid injection of 0.2 ml of 1/40 diluted CB solution. The linearity and stationarity of the vascular system, reproducibility of the curve, and recirculation were tested. The tissue CB dilution curve, a cumulative distribution function, was first differentiated yielding two curves comprising the inlet and outlet curves. The error due to "overlapping" was corrected for by extrapolation using the gamma-density function. The transfer function through the cortex was then calculated by numerical deconvolution of the inlet and outlet curves. The validity of the calculated transfer function was verified by comparison with that obtained from actual CB dilution curves recorded simultaneously from the pial artery and superior sagittal sinus. The transfer function was further subjected to moment analysis for determining the intraparenchymal circulatory parameters of the cerebral cortex. Some examples demonstrating the applicability of technique are given.

Animals↗
PubMed1983

Effects of hemolysis, hematocrit, RBC swelling, and flow rate on light scattering by blood in a 0.26 cm ID transparent tube.

The intensity of light scattering by blood in a tube of diameter 0.26 cm was measured with an apparatus devised by us at different angles on an incident cross-sectional plane. Changes in angular distribution of light intensity associated with hemolysis, and changes in hematocrit (Ht), red blood cell (RBC) swelling, and flow rate were plotted on polar coordinates. The dyssymmetry index, defined as the ratio of the intensity of light at 45 degrees to that at 135 degrees, was used to characterize the shape of the diagrams of light scattering. The index decreased with Ht, flow rate, but increased with RBC swelling. It is concluded that light scattering by blood requires intactness of the RBC membrane. Even when the cell membrane is intact, light scattering is subject to changes with the flow rate of blood, presumably due to RBC aggregation.

Animals↗
PubMed1982

Video camera method for simultaneous measurement of blood flow velocity and pial vessel diameter.

A new method for the simultaneous measurement of blood flow velocity and pial vessel diameter is described. The system consists basically of a high-sensitivity vidicon camera, camera control, width analyzer, video densitometer, TV monitor, desktop computer, and multi-pen recorder. The pial vessels are visualized through a cranial window at 25-200x magnification on the TV monitor. The diameter of three target vessels can be recorded simultaneously on the recorder by adjustment of controllable video signal gates using the width analyzer. At the same time, the optical densities of two targets at points upstream and downstream of the pial vessel are measured continuously with video densitometers, and their outputs are recorded on the polygraph and analyzed by the computer. The time difference in the two peaks of time--concentration curves, produced very 2-3 s at the highest frequency by the injection of a small amount of saline through the lingual artery, is measured on-line using the computer. The flow velocity in the vessel is calculated from the time difference and the distance between the two targets. The system was shown to be stable, reliable, and rapid in response. This method may provide a useful tool for research in the field of blood circulation in the brain or any other organ.

Animals↗
PubMed1982

Diagnosis of carcinoma of the pancreas by assay of immunosuppressive acidic protein.

To establish a procedure for screening of pancreatic cancer, we assayed the serum level of immunosuppressive acidic protein (IAP) in patients with malignant and benign pancreatic diseases and compared it with the serum level of carcinoembryonic antigen (CEA). In 96% of 46 pancreatic cancer patients, the serum IAP level averaged 928 micrograms/ml; while 500 micrograms/ml or below was obtained as the normal value in 211 healthy adults. The serum IAP level may be elevated in the presence of non-malignant, inflammatory pancreatic diseases. For the differential diagnosis of pancreatic cancer, therefore, other laboratory data need to be taken into account. The serum IAP level was correlated with the progression of pancreatic cancer and may provide a means to determine whether tumor resection was radical or admitting of recurrence. The serum IAP level in samples obtained from different sites of the portal system did not reflect tumor location in patients with pancreatic cancer.

Adolescent↗