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Biomedical subjects

M Klockars

Publications and source records attributed to M Klockars.

121 records · Page 7Linked to original sources
PubMedDate not supplied

Effect of two particle surface-modifying agents, polyvinylpyridine-N-oxide and carboxymethylcellulose, on the quartz and asbestos mineral fiber-induced production of reactive oxygen metabolites by human polymorphonuclear leukocytes.

We studied the capacity of quartz and asbestos fibers to induce the generation of reactive oxygen metabolites in human polymorphonuclear leukocytes (PMNs) with a chemiluminescence (CL) assay. On an equal weight basis, the particulates induced CL in the following order of magnitude: chrysotile, quartz greater than amosite, crocidolite, greater than anthophyllite, wollastonite. The intensity of CL correlated positively with the Alcian blue (a cationic dye) binding capacity of the particles. Polyvinylpyridine-N-oxide (0.5 microgram/ml) inhibited completely the CL induced by quartz but had little effect on the CL induced by asbestos fibers. Carboxymethylcellulose (1.0 microgram/ml), however, reduced the CL caused by chrysotile asbestos but had no effect on the CL induced by the other particulates. Our results suggest that in addition to length and diameter, the effect of quartz and asbestos fibers on inflammatory cells will depend on surface characteristics, including the charge of the particles.

Adult↗
PubMedDate not supplied

Human lysozyme (origin and distribution in health and disease).

Histochemical and in particular immunoperoxidase techniques have extended our knowledge about the cellular distribution of LZM. The enzyme has been detected in: 1. granulocytes, which synthesize LZM in bone marrow; 2. cells of the mononuclear phagocytic system, which synthesize LZM continuously; 3. various exocrine glands, whose LZM is probably in part synthesized locally and in part derived from blood; 4. cartilage, which probably synthesizes LZM and 5. the kidney, in which LZM is serum-derived. In pathological conditions, such as inflammation and neoplasia, most LZM-positive cells at the site of disease are either granulocytes or members of the mono-nuclear phagocytic system. A notable exception are the metaplastic Paneth cells that occur along the gastrointestinal tract in inflammation and neoplasia. LZM is an antibacterial agent, but evidence is accumulating which suggests that the enzyme might have other functions as well.

Animals↗