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Biomedical subjects

M Kikuchi

Publications and source records attributed to M Kikuchi.

At least 901 records · Page 50Linked to original sources
PubMed1987

Cytomegalovirus infections in adult T-cell leukemia patients.

Cytomegalovirus (CMV) infection frequently occurred in patients with malignant lymphoma of T-cell origin, especially with adult T-cell leukemia (ATL). This was evidenced by histopathological examination at autopsy, isolation of CMV, and detection of CMV antibodies that indicate recent or active infection. Cellular immune response was suppressed in most ATL patients when examined by skin hypersensitivity reaction to purified protein derivatives (PPD), streptococcal antigens (SuPs), and phytohaemoagglutinin (PHA). None of the CMV-positive patients reacted to them. Thus, the presence of tumor cells of T-cell origin and the absence of skin hypersensitivity reaction seem to be risk factors for CMV infection. Each CMV isolate exhibited unique DNA fingerprints, suggesting that cross-infection of CMV did not occur among the ATL patients on the same ward.

Antibodies, Viral↗
PubMed1987

The effect of recombinant erythropoietin on murine megakaryocyte colony formation.

We investigated the effect of a recombinant human erythropoietin preparation (recombinant Epo) on murine megakaryocyte (MK) colony formation in serum-free and serum-containing culture systems, in order to study the relationship between Epo and megakaryopoiesis. Pokeweed mitogen spleen-conditioned medium (PWM-SCM), a standard source of MK colony stimulator, dose-dependently stimulated MK colony formation in the two culture systems. The plating efficiency of serum-free cultures was almost equal to that of cultures containing serum. Recombinant Epo also dose dependently stimulated MK colony formation in serum-containing cultures. However, in serum-free cultures recombinant Epo alone did not stimulate the growth of MK colonies; with the addition of fetal calf serum (FCS) to the serum-free cultures, recombinant Epo induced the growth of MK colonies. Furthermore, recombinant Epo enhanced MK colony formation through the stimulation of PWM-SCM or murine interleukin 3 (IL-3) in serum-free cultures. Our data show that Epo can act as a stimulator of megakaryopoiesis in collaboration with a factor in serum, or with an MK colony stimulator such as IL-3.

Animals↗
PubMed1987

Partial ornithine transcarbamylase deficiency in females: diagnosis by an immunohistochemical method.

Females heterozygous for the X-linked urea cycle disorder, ornithine transcarbamylase (OTC) deficiency have a significant risk of developing hyperammonaemia. Diagnosis of this genetic defect in a proband is the essential starting point for family studies. By an immunohistochemical analysis of the liver specimens fixed in 10% formalin, we confirmed heterozygous status for OTC deficiency in two female patients, a 15-year-old girl and a 2-year-old girl, who died of hyperammonaemia. Since most affected males lack cross reactive materials (CRM), an immunochemical analysis should be useful for the diagnosis of most heterozygous females.

Adolescent↗
PubMed1987

Diabetes induced by neonatal streptozotocin treatment in spontaneously hypertensive and normotensive rats.

The development of non-insulin-dependent diabetes mellitus (NIDDM) induced by neonatal streptozotocin (STZ) treatment was compared between male spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto rats (WKY). The animals were intraperitoneally given 37.5, 50.0, 62.5, or 75.0 mg/kg of STZ at two days of age. At two days after STZ injection, plasma glucose was elevated in both groups of rats according to the dose of STZ, but the level was higher in SHR than in corresponding WKY. At ten days of age, plasma glucose in WKY returned to the similar level to that in vehicle-treated control irrespective of the doses of STZ, while in SHR it remained above control and its level was significantly higher than that in WKY. At 12 weeks of age, plasma glucose was within the control range in WKY, while in SHR it was markedly and dose-dependently elevated. The present study indicates that SHR are susceptible to NIDDM induced by neonatal STZ treatment. The difference in response to STZ between SHR and WKY was discussed.

Aging↗
PubMed1987

Bioconcentration of alcohol ethoxylates in carp (Cyprinus carpio).

The uptake, distribution, and clearance of three labeled nonionic surfactants, 14C-labeled dodecyl tetra(oxyethylene) ether [14C-C12-AE(4)], 14C-labeled dodecyl octa(oxyethylene) ether [14C-C12-AE(8)], and 14C-labeled dodecyl hexadeca(oxyethylene) ether [14C-C12-AE(16)] were investigated in carp (Cyprinus carpio) exposed to concentrations of 0.2-0.6 mg X liter-1, using whole-body autoradiography and the liquid scintillation counting method. 14C radioactivity was rapidly absorbed into the fish body and distributed in skin, nasal and oral cavities, gills, brain, hepatopancreas, kidney, gall bladder, and intestinal content at comparatively high concentrations. The calculated wet weight whole-body bioconcentration factor at steady state in the fish exposed to 14C-C12-AE(4), 14C-C12-AE(8), or 14C-C12-AE(16) for 72 hr was 310, 220, or 4.3, respectively. Clearance of 14C radioactivity from the fish body was rapid, with half-lives of 30-80 hr. The metabolites of 14C-C12-AE(4) were also examined in gills, blood, kidney, hepatopancreas, or gall bladder by thin-layer chromatography.

Animals↗
PubMed1987

Expression of human lysozyme in an insoluble form in yeast.

A high level of expression in yeast of a chemically synthesized human lysozyme (hL) gene was achieved by introducing an A-rich DNA fragment just upstream from the ATG start codon. The synthesized recombinant human lysozyme (r-hL) was insoluble and biologically inactive. It was solubilized with 7 M urea (pH 9) from yeast cells and its lytic activity was efficiently regenerated by oxidative renaturation. This renaturation experiment and Western blotting analysis under reducing and non-reducing conditions indicate that the insoluble form might be caused by the formation of incorrect intra- or intermolecular disulfide bonds. The N-terminal amino acid sequence of the purified r-hL was identical with that of authentic hL.

Amino Acid Sequence↗
PubMed1987

Sex differences in diabetes induced by neonatal streptozotocin treatment in spontaneously hypertensive rats.

We examined the sex differences in the development of diabetes due to neonatal streptozotocin (STZ) treatment in spontaneously hypertensive rats (SHR) which are more prone to diabetes than normotensive rats. Male and female SHR were intraperitoneally injected with various doses of STZ at 2 days after birth. In those treated with vehicle or 25 mg/kg of STZ, there were no sex differences in plasma glucose levels, which changed little during the 12 weeks of observation. When treated with 50 mg/kg of STZ, however, male SHR developed overt hyperglycemia greater than 300 mg/dl plasma glucose after 8 weeks of age, while females showed a minimal change in plasma glucose levels. When given 75 mg/kg of STZ, female rats developed overt hyperglycemia at 4-6 weeks of age, during which time male SHR showed no apparent hyperglycemia. At 8 weeks or later, however, both males and females had similarly high levels of plasma glucose. Glycosylated hemoglobin at 12 weeks was compatible with plasma glucose levels in each group. The present results indicate that there are sex differences in susceptibility to neonatal STZ treatment and in development of hyperglycemia.

Aging↗
PubMed1987

Intermediate filaments of myofibroblasts. Immunochemical and immunocytochemical analyses.

We generated a monoclonal anti-vimentin antibody, VIM-1, by mouse hybridoma technique, using an established myofibroblast line as a whole cell immunogen. The presence of vimentin polypeptides in the cultured myofibroblasts was confirmed by SDS-polyacrylamide gel electrophoresis and immunoblotting. By light microscopic immunocytochemistry, myofibroblasts in cultures as well as in frozen tissue sections showed a strong reaction with the anti-vimentin antibody, whereas these cells lacked either detectable desmin or cytokeratin. Our results support the fibroblastic origin of myofibroblasts. Immunoelectron microscopic study with ferritin-ABC technique demonstrated that VIM-1 reacted exclusively with 10-nm intermediate filaments, while other cellular structures revealed uniformly negative reaction against the antibody.

Antibodies, Monoclonal↗
PubMed1987

A profile of plasma branched chain amino acids in a totally pancreatectomized patient: effects of glucagon replacement under a steady feeding state.

Physiological amounts of glucagon replacement brought about the reduction of plasma branched chain amino acid levels as well as glucogenic amino acid levels in a totally pancreatectomized patient who was under a steady feeding state, i.e., constant administration of an elemental diet combined with continuous subcutaneous insulin infusion. This finding suggests that glucagon may play a physiological role not only on glucogenic amino acids but also branched chain amino acids in plasma in feeding state.

Amino Acids, Branched-Chain↗
PubMed1987

Histopathology and immunohistochemistry of peripheral T cell lymphomas: a proposal for their classification.

Based on the results of histological and immunohistochemical observations of a large number of peripheral T cell lymphomas from China, England, Germany and Japan, histological and cytological morphology were correlated with immunophenotype, aetiological association with HTLV-1, and clinical behaviour to produce a working classification of the T cell lymphomas. This classification, based mainly on cytological criteria, divides the peripheral T cell lymphomas into tumours of low grade and high grade malignancy. Adult T cell lymphoma/leukaemia (ATLL) is caused by HTLV-1 and belongs chiefly to the high grade category. Some tumours are characterised by an admixture of other cells (epithelioid cells, follicular dendritic cells, etc) and structures (high endothelial venules, follicles), which may indicate the secretion of lymphokines by the tumour cells. Clear cells seem to be specific for T cell lymphomas and may occur in various types of peripheral T cell lymphoma.

Adult↗
PubMed1987

Studies on a new antiallergic pyridinecarboxamide TA-5707F and its sodium salt (TA-5707). II. Mechanism of antiallergic action of TA-5707.

Mechanism of the antiallergic action of 6-methyl-N-(1H-tetrazol-5-yl)-2-pyridinecarboxamide (TA-5707F) was studied using the water-soluble sodium salt (TA-5707). 1) TA-5707 administered p.o. at the dose ca. 3 times the ID50 for the PCA reaction did not inhibit capillary dye leakage induced on the rat skin by intracutaneous injection of histamine, serotonin, bradykinin and leukotriene D4 (LTD4). 2) TA-5707, at concentrations above 10(-7) M, inhibited antigen-induced histamine release and dye leakage in the rat peritoneal cavity (passive peritoneal anaphylaxis). 3) TA-5707 inhibited both anaphylactic and compound 48/80-induced histamine release from the rat peritoneal mast cells, the IC50 being ca. 10(-5) M in both cases. It was concluded that TA-5707F exerts its antiallergic action by inhibiting the release of chemical mediators from sensitized cells.

Animals↗
PubMed1987

Blood pressure changes in spontaneously hypertensive and normotensive rats with neonatal streptozotocin induced type 2 diabetes.

We examined the blood pressure changes in hypertensive and normotensive rats with Type 2 diabetes induced by neonatal streptozotocin (STZ) treatment. STZ was intraperitoneally injected at 2 days of age with the dose of 25, 50 and 75 mg/kg for male spontaneously hypertensive rats (SHR) and with 75, 100, 125 and 150 mg/kg for male normotensive Wistar Kyoto rats (WKY). Blood pressure was measured by indirect tail-cuff method until 12 weeks of age. STZ-treated SHR, of which plasma glucose and glycosylated hemoglobin increased and body weight decreased with the dose of STZ, developed and maintained hypertension, same as did the vehicle-treated control SHR. On the other hand, STZ-treated WKY which developed only mild hyperglycemia lost body weight with the dose of STZ but the blood pressures rose slightly, these changes being correlated with the glycemic levels. The explanation for these differences between SHR and WKY remained to be elucidated.

Animals↗
PubMed1987

Malignant fibrous histiocytoma. Evidence of perivascular mesenchymal cell origin immunocytochemical studies with monoclonal anti-MFH antibodies.

Using whole cell antigens prepared from the established lines of human malignant fibrous histiocytoma (MFH), the authors have generated two different monoclonal antibodies (FU3 and FU4) by a mouse hybridoma technique. By indirect immunoperoxidase in frozen tissue sections, FU3 and FU4 revealed strong staining of perivascular mesenchymal cells and fibroblasts. In the spleen FU3 stained perivascular cells of the ellipsoids and the marginal zone of the lymph follicles. Macrophages in granulation tissues as well as monocytes and other blood cells in normal peripheral blood were uniformly negative for the antigen detected by FU3. Among various soft-tissue tumors, MFH and liposarcoma reacted strongly with FU3 and FU4, but synovial sarcoma revealed no reaction with either of the antibodies. Immunoelectron-microscopic studies demonstrated positive reactions with FU3 and FU4 on the surface of MFH cell membrane, which suggests that these antibodies recognized cell surface antigens. In conclusion, MFH shares antigenicity with perivascular mesenchymal cells and fibroblasts as well as liposarcoma. MFH and liposarcoma may have a common origin from the perivascular mesenchymal cells that are supposed to have a potential for multidirectional differentiation.

Animals↗