Search PubMed⌕ Search

Biomedical subjects

M Kikuchi

Publications and source records attributed to M Kikuchi.

At least 649 records · Page 36Linked to original sources
PubMed1993

Purification and identification of an angiotensin I-converting enzyme inhibitor from soy sauce.

The inhibitory activity of an angiotensin I-converting enzyme (ACE) detected in soy sauce was fractionated into two major fractions of high molecular weight (Hw) and low molecular weight (Lw) by gel filtration chromatography on Bio-gel P-2 after treating with ethanol. The Hw fraction reduced the blood pressure in hypertensive rats after orally administering, while the Lw fraction did not. The ACE inhibitor in the Hw fraction was further purified by Dowex 50W ion-exchange chromatography and four subsequent steps of HPLC. On the basis of the SIMS-mass spectrum, NMR spectrum and other characteristics, the purified ACE inhibitor was identified as nicotianamine (N-[N-(3-amino-3-carboxypropyl)-3-amino-3-carboxypropyl]azetidine-2- carboxylic acid). The IC50 value for this ACE was 0.26 microM.

Angiotensin-Converting Enzyme Inhibitors↗
PubMed1993

Isolation and characterization of a novel immunomodulating fraction from soybeans.

A novel immunomodulating fraction, SP-MAF1, was isolated from soybeans as a polysaccharide-protein complex by hot water extraction followed by acid treatment, ethanol treatment, and chromatography on a DEAE-Sepharose CL-6B column, and was characterized in some biological activities. SP-MAF1 increased glycolysis and IL-1 production by macrophages, but did not affect TNF production. SP-MAF1 also increased splenocyte proliferation that was induced by LPS and ConA, and ConA-induced production of IL-2 by splenocytes. In an in vivo test, the mean survival times of SP-MAF1-treated mice bearing FM3A were significantly longer than those of non-treated group. Since SP-MAF1 affected macrophages and splenocytes but not tumor cell growth, this effect may be mediated by the host immune system. Furthermore SP-MAF1 had a capacity to suppress the TNBS-induced DTH reaction. These results suggested that SP-MAF1 could either enhance or suppress immune functions.

Adjuvants, Immunologic↗
PubMed1993

Pathological extent of interventricular septal infarction in patients with acute anteroseptal myocardial infarction with and without right bundle branch block.

The purpose of the present study is to elucidate the difference in extent of myocardial infarction (MI) between those cases with and those without right bundle branch block (RBBB) occurring during the course of acute MI. We examined postmortem hearts from 20 patients with acute anteroseptal MI; 10 with (group A) and 10 without RBBB (group B). The extent of MI was studied pathologically in the interventricular septum (IVS) and reconstructed. The longitudinal extent of MI did not distinctly differ between groups A and B. In 5 hearts of group B, the anterior limit of the MI extended as high, or as close to the cardiac base, as in group A hearts. Transmural MI was relatively common and seen more frequently in group A than in group B. However, the MI did not always extend evenly to the left and right ventricular sides in the IVS. Left-sided predominance in extent of the MI was more frequently observed in group B than in group A. Right-sided predominance in the extent of septal MI was relatively rare and was seen in 5 cases at the portion where the right bundle branch came down. Four of these were group A patients. Thus, involvement of the right bundle branch might partly depend on the unevenness of the mural extent of the MI as well as the height of extension of the MI in the IVS.

Aged↗
PubMed1993

Acute lymphoblastic leukemia associated with a t(1;19)(q23;p13) in an adult.

A cytogenetic study of a 38-year-old patient with acute lymphoblastic leukemia (ALL) revealed a t(1;19)(q23;p13), which is a characteristic translocation of childhood ALL. The leukemic cells were positive for the CD10, CD19, HLA-DR, TdT and cytoplasmic mu-chain. Both of the immunoglobulin heavy chain gene loci were rearranged and the RNA-based polymerase chain reaction demonstrated the E2A/PBX1 fusion transcript which is the result of the t(1;19). This finding suggests that the t(1;19) is implicated not only in childhood ALL but also in adult ALL patients.

Adult↗
PubMed1993

Superficial and protruding type of esophageal adenocarcinoma mucocellulare and muconodulare confined mostly to the lamina propria mucosa and partly in the submucosal layer.

We report a case with an adenocarcinoma mucocellulare and muconodulare of the esophagus which is limited to the lamina propria mucosa and the submucosal layer. The patient, a 74-year-old man had the chief complaints of hematemesis and melena. A superficial and protruding (0-I) type of lesion was found at the lower thoracic portion of the esophagus by X-ray and endoscopic examination. The surgically resected esophageal specimen showed 0-I and predominantly subepithelial (Sep) type. The tumor is presumed to have arisen from the cardiac glands in the lamina propria mucosa of the lowermost region of the esophagus.

Adenocarcinoma↗
PubMed1993

The state of the art of CO laser angioplasty system.

A unique percutaneous transluminal coronary angioplasty system using new infrared therapy laser with infrared glass fiber delivery under novel angioscope guidance was described. Carbon monoxide (CO) laser emission of 5 mm in wavelength was employed as therapy laser to achieve precise ablation of atheromatous plaque with a flexible As-S infrared glass fiber for laser delivery. We developed the first medical CO laser as well as As-S infrared glass fiber cable. We also developed 5.5 Fr. thin angioscope catheter with complete directional manipulability at its tip. The system control unit could manage to prevent failure irradiations and fiber damages. This novel angioplasty system was evaluated by a stenosis model of mongrel dogs. We demonstrated the usefulness of our system to overcome current issues on laser angioplasty using multi-fiber-catheter with over-the-guidewire system.

Angioplasty, Balloon, Laser-Assisted↗
PubMed1993

Bone marrow findings in malignant histiocytosis and/or malignant lymphoma with concurrent hemophagocytic syndrome.

We examined bone marrow specimens from 19 patients with malignant histiocytosis (MH) and/or malignant lymphoma (ML) with concurrent hemophagocytic syndrome (HS) who suffered from high fever, hepatosplenomegaly, liver dysfunction, profound cytopenia, and erythrophagocytosis. There was little lymph-node enlargement or no tumor formation. The neoplastic cells in 3 patients exhibited histiocytes/macrophages phenotype with positive reactions for fluoride-sensitive nonspecific esterase, lysozyme and CD68 (KP1). Twelve other patients showed a T-cell (CD3) phenotype, in which 5 patients expressed CD30 (BerH2) as well. B-cell characteristics with CD20 (L26), CIg. nu lambda and gamma kappa were manifest in 2 patients, but indeterminate markers were found in the 2 remaining patients. Eighteen patients showed an infiltration of large neoplastic cells mainly with noncohesive interstitial growth pattern, ranging from 1.7% to 74.2% of the nucleated cells in the bone marrow. A large number of histiocytes/macrophages and dendritic cells was diffusely observed in 15 patients. Severely decreased hematopoiesis in all three series of hematopoietic cells was found in 16 patients. Bone marrow infiltration by the neoplastic cells and numerous reactive cells with erythrophagocytosis appears to be an important factor of profound cytopenia in patients of MH and/or ML with HS. The infiltrating pattern of the neoplastic and reactive cells in the bone marrow of MH and/or ML with HS was different from that of other types of peripheral T-cell ML, B-cell ML in high grade malignancy, and Hodgkin's disease. Cell characteristics and lineage of the neoplastic cells in MH and/or ML with HS are also discussed in this study.

Adolescent↗
PubMed1993

Sinonasal malignant lymphoma of natural killer cell phenotype associated with diffuse pancreatic involvement.

We report a case of sinonasal lymphoma with a natural killer (NK) phenotype. This 40-year-old man was admitted to our hospital because of left nasal obstruction. Physical examination and computed tomography of the skull revealed a tumor in the left nasal cavity and maxillary sinus. Histopathological examination revealed a diffuse proliferation of pleomorphic lymphoid cells. Imprint cytology showed that tumor cells contained some azurophilic granules, and expressed CD2, CD8, CD16, CD56 and HLA-DR antigens with little expression of other lymphoid or myeloid markers. Southern blot analysis revealed germline configuration for immunoglobulin heavy chain and T-cell receptor genes. These findings indicated that these cells were in fact NK cells. The patient's enlarged pancreas was also involved by lymphoma and the pattern of involvement simulated that seen in primary pancreatic lymphoma. Ulcerative colitis (UC) was also present, a rare finding in this disorder.

Adult↗
PubMed1993

[Immunoblotting analysis of acquired factor VIII inhibitor in an elderly man].

A case of acquired FVIII inhibitor in a nonhemophilic, 80 year-old man who had no underlying disorder, is presented. The level of inhibitor was 13.3 Bethesda unit/ml, and the antibody was IgG, with lambda light chain. The antibody reacted with the 92 kD fragment of non-treated human FVIII, and the 44 kD fragment of thrombin treated FVIII when analysed by the immunoblot analysis.

Aged↗
PubMed1993

Tumor induction in mice administered neonatally with N-bis(2-hydroxypropyl)nitrosamine (BHPN).

Two subcutaneous injections of N-bis(2-hydroxypropyl)nitrosamine (BHPN) to mice on day 1 and 8 induced tumors of the lung, liver, nasopharynx and pancreas, when animals were killed at the end of one year. The tumor incidences of lung were 90-100%; those of liver 60-92%; those of nasopharynx 3-13%; those of pancreas 2-3%, respectively. The average tumor nodules of lung and those of liver increased in a dose-dependent manner. Two doses of 50 mg/g body weight of BHPN induced one duct cell carcinoma and one acinar cell adenoma of pancreas in a mouse, respectively.

Animals↗
PubMed1993

Blood velocity in the ophthalmic artery determined by color Doppler imaging in normal subjects and diabetics.

Color Doppler imaging was used to analyze the blood velocity in the ophthalmic artery (BVA) of 33 normal subjects and 36 diabetic patients. Maximum systolic BVA (Vmax), minimum end-diastolic BVA (Vmin) and Pourcelot index (RI: index of vascular resistance) were determined by analyzing the pulse wave of flow velocity in the ophthalmic artery. The coefficient of reproducibility of Vmax, Vmin and RI in normal subjects was 10%, 9% and 4%, respectively, when analyzed twice at one-hour intervals. There was no significant difference in the indices of the right eyes compared to those of the left eyes. The averages of Vmax, Vmin and RI were 25.4 +/- 7.6 cm/s, 6.3 +/- 2.3 cm/s and 0.75 +/- 0.052 (mean +/- SD), respectively. Vmax and Vmin decreased significantly with age (Vmax: r = -0.65, P < 0.001; Vmin: r = -0.61, P < 0.001). RI did not change with age. Vmax, Vmin and RI were compared between normal subjects and patients. RI was significantly higher in diabetics (0.775 +/- 0.047 in patients without retinopathy, 0.779 +/- 0.084 in patients with background retinopathy and 0.786 +/- 0.081 in patients with preproliferative or proliferative retinopathy) than in normal subjects (0.728 +/- 0.054). This suggests there is a pathological increase in choroidal vascular resistance and/or a decrease in the diameter of the ophthalmic artery in diabetics.

Adult↗
PubMed1993

Enhancing effects of IL-2 on M-CSF production by human peripheral blood monocytes.

Monocytes have the capacity to produce granulopoietic factors such as M-CSF and G-CSF. Our findings here showed that interleukin-2 (IL-2), at a concentration of more than 0.05 ng/ml, led to a 12-fold increase in the production of M-CSF in the human peripheral blood mononuclear cells after 72 h incubation compared to the control culture. Even in purified monocyte cultures with added IL-2, a 3-fold increase in M-CSF production was observed at an IL-2 concentration of 50 ng/ml. The enhancing effect of IL-2 on M-CSF secretion was also observed when IL-2-stimulated non-phagocytic cell-conditioned medium was added to monocyte cultures. These results indicated that IL-2, both directly and indirectly, activated monocytes to enhance the production of M-CSF. In addition, the expression of IL-2 receptor (CD25) on monocytes was more enhanced in cultures containing IL-2 than in cultures without IL-2. On the other hand, IL-2 did not induce G-CSF production in purified monocytes. These in vitro results suggest that when IL-2 is used clinically, the various biological activities of M-CSF should also be taken into consideration.

Cells, Cultured↗
PubMed1993

[Effect of cigarette smoking on diffuse pulmonary uptake of 99mTc-HM PAO].

To investigate the clinical significance of pulmonary uptake of 99mTc-HM PAO, we studied the effect of cigarette smoking on pulmonary uptake of 99mTc-HM PAO. This study included 14 volunteers: 4 nonsmokers and 10 smokers (Brinkmann indexes 544 +/- 270.8). Following injection of 99mTc-HM PAO into the antecubital vein, time-activity curve was obtained with the regional activity of one frame/min. for 30 minutes and 64 x 64 matrices. Characteristics of pulmonary clearance curve, described as C(t) = A exp-kt, and lung/liver uptake ratio (Lu/Li index) at 30 min. after injection were determined, and groups of non-smokers and smokers were compared. In smokers, 99mTc-HM PAO clearance was delayed and k was smaller significantly. Lu/Li index was also higher in smokers. Brinkmann index correlated both with k (r = -0.815, p < 0.01) and Lu/Li index (r = 0.911, p < 0.01). In conclusion, the delayed clearance and retention of 99mTc-HM PAO in the lung may indicate metabolic disorders of the lung due to cigarette smoking.

Adult↗
PubMed1993

[Within-day and day-to-day variations of serum M-CSF levels in healthy volunteers].

We have investigated on within-day and day-to-day variations of serum M-CSF levels in healthy volunteers (ranges of age: 20-21 years old). M-CSF levels in serum were measured using enzyme-linked immunosorbent assay (ELISA). Serum M-CSF levels in early morning were 1.31 +/- 0.30 ng/ml (mean +/- SD, n = 10) in male volunteers, 1.50 +/- 0.13 ng/ml (n = 10) in female volunteers. No significant difference was observed between male and female volunteers in serum M-CSF levels. No significant within-day variations in serum M-CSF levels were also observed. Serum M-CSF levels in period of thirteen days in each individual person were almost constant (n = 5). The inverse correlation was observed between M-CSF levels and number of neutrophils. The correlation, however, was not observed between M-CSF levels and number of monocytes. The present results suggest that there are mechanisms in vivo which maintain serum M-CSF in a constant level in each individual person.

Adult↗