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Biomedical subjects

M Kietzmann

Publications and source records attributed to M Kietzmann.

At least 73 records · Page 4Linked to original sources
PubMed1988

Investigation of the skin thinning effect of prednicarbate and other corticoids in mouse skin.

In this investigation skin fold thinning was determined after topical application of several potent corticosteroids in hairless mice using a simple mechanical measuring device. The skin thinning effect of prednicarbate was compared with other corticosteroids (amcinonide, beta-methasone-17-valerate, clobetasol-17-propionate, diflorasone-17,21-diacetate, hydrocortisone-21-acetate). Prednicarbate produced a clear thinning of skin. Like other tested dermatocorticoids prednicarbate caused a significant atrophy of the mouse tail epidermis. By prednicarbate the 3H-thymidine triphosphate incorporation into epidermal DNA was inhibited.

Adrenal Cortex Hormones↗
PubMed1988

Effects of treatment with dithranol, etretinate and a combination of dithranol and etretinate on epidermal metabolism and histology. Mouse tail assay.

The mouse tail assay is useful for studying dermatological therapeutics that influence epidermal differentiation. The effect of dithranol (0.1% w/v in acetone, topically; DI), etretinate (2 mg/kg body weight/d, orally; ET) and of the simultaneous administration of both preparations (DI/ET) on the epidermis of the mouse tail was examined. The thickness of the epidermis, the number of nuclei per defined epidermal section, and the development of a granular layer in the interfollicular areas were determined. The incorporation of 3H-thymidine-triphosphate onto the DNA and global protein synthesis were also determined in vitro. The incorporation rates of leucine and histidine into protein in vitro were measured separately. DI caused retention acanthosis and at the same time orthokeratotic differentiation of the interfollicular areas of the epidermis. Light microscopy of the tail epidermis after application of ET showed no significant changes. Administration of ET/DI yielded the same effects as treatment with DI alone. DI partially reduced the biochemical parameters. ET increased DNA-polymerase activity, global protein synthesis, and the incorporation rate of histidine. The biochemical results after treatment with DI/ET were very similar to those obtained after DI administration. From the histological and biochemical results it is therefore concluded that the simultaneous administration of DI and ET produces the same effects as DI treatment alone.

Animals↗
PubMed1988

Effects of etretinate in epidermal metabolism and histology after abrasio of superficial layers. Mouse tail assay.

The mouse tail assay is suitable for the histological detection of altered differentiation of the epidermis. In the present study, the stratum corneum of the tail epidermis of female mice (NMRI) was removed by means of fine sandpaper. One group of mice was treated with 2 mg of ethyl (all-trans)-9-(4-methoxy-2,3,6-trimethylphenyl)-3,7-dimethyl- 2,4,6,8-nonatetraenoate (etretinate)/kg body weight daily. The untreated group served as control. After removal of the stratum corneum epidermal hyperplasia and formation of a stratum granulosum was found in the controls (peak on the third day). The alterations declined from the fourth day onwards. The histidine incorporation into protein was increased from days 4 to 6. A similar reaction sequence was observed in the animals treated with etretinate. In addition to the hyperplasia, there was an enlargement of the cells (hypertrophy). The control group showed a significant elevation of leucine incorporation and a reduction of histidine incorporation into protein on the first day after removal of the stratum corneum. The incorporation rate of thymidine triphosphate into DNA peaked on the second day. The rise of leucine incorporation into protein and the increase of thymidine triphosphate incorporation into DNA on the second day after removal of the stratum corneum could not be detected in the animals treated with etretinate. In these animals histidine metabolism could not be influenced by etretinate. In conclusion, etretinate greatly influences the alteration of basal metabolism caused by the removal of the stratum corneum.(ABSTRACT TRUNCATED AT 250 WORDS)

Amino Acids↗