Biomedical subjects
M Kelly
Publications and source records attributed to M Kelly.
Localization with xeromammography.
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Non-Hodgkins lymphoma involving the optic nerve.
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Variation of macrophage migration by a factor from regional lymph node cells of breast cancer patients.
Using a standard heterologous assay in which lymphokines alter guinea pig peritoneal macrophage migration, it was found that regional lymph node cells (RLNCs) from patients with primary breast cancer elaborate soluble factors which variably affect such migration. Variation of migration resulted when soluble factors employed were obtained from different nodes in the same patient as well as from nodes from different patients. Some nodes from a patient elaborated migration inhibition factors (MIF) and other migration enhancing factors (MEF). The findings are in keeping with others obtained by us relative to the variation in lymphocyte transformation and thymidine uptake by RLNCs and further emphasize that all RLNs in patients with breast cancer are not biologically similar. They lend support to our previous hypothesis that the reason why some RLNs contain metastases and others do not is more likely due to biological differences than because of anatomical happenstance, i.e., transport of tumor cells to some nodes and not to others.
Leukocyte beta-galactosidase activity in the diagnosis of bovine GM1 gangliosidosis.
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Effects of sublethal concentrations of cadmium on adult Palaemonetes pugio under static and flow-through conditions.
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Na+ transport in jejunal crypt cells.
As enterocytes migrate from crypts to villi they differentiate and mature. To examine the effect of epithelial differentiation on ion transport we studied 22Na+ efflux and (Na+--K+)-adenosine triphosphatase activity in suspensions of epithelial cells selectively isolated from different regions of the villus to compare crypt cells with villous tip cells. Enterocytes were isolated from rat jejunum by a dilation-vibration technique. Thymidine kinase, sucrase, and alkaline phosphatase activities were measured as markers of specific cell populations. Compared to villous cells, cells from the crypt region demonstrated lower (Na"--K+)-adenosine triphosphatase activity, lower total and passive Na+ efflux rate constants, and failure of Na+ transport to respond to an actively transported nonelectrolyte.
Letter: Medical Information bureau.
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A further investigation of the relationship between serum diamine oxidase (DAO) and gestational age.
A standard error of one to two weeks must be accepted if the duration of pregnancy is calculated from serum diamine oxidase (DAO) concentrations. After the 16th week of pregnancy, the enzyme concentration is too variable to be used for calculations of gestational age.
Role of temperature-sensitive mutants in persistent infections initiated with vesicular stomatitis virus.
Noncytocidal persistent infections at 37 C of mouse L cells (Lvsv) with infective B particles of vesicular stomatitis virus (VSV) could be established only in the presence of large numbers of defective interfering (DI) particles. Under these conditions, there was a rapid spontaneous selection of temperature-sensitive (ts) virus. At 10 days there was an increase to 17.8% in the frequency of ts clones in the virus population; by 17 days this frequency had reached 85.2%, and by 63 days 100% of the clones isolated were ts at 39.5 C, the nonpermissive temperature used. All 34 of the clones isolated from the 84-day fluid had an RNA-phenotype, and 8 clones that were tested all belonged to VSV complementation group I. When tested by an interference assay, Lvsv fluids did not contain significant numbers of DI particles (less than 1 DI/PFU). Furthermore, persistent infection of L cells at 37 C could be initiated under conditions in which few, if any, DI particles were present by using low input multiplicities (10(-4) and 10(-5) of a clonal isolate of an RNA-group I mutant obtained from Lvsv cells. On the basis of these and other results, a mechanism is proposed to explain the role of ts mutants in both the establishment and maintenance of the persistently infected state.
Tuberoinfundibular neurons: dopaminergic and norepinephrinergic sensitivity.
The techniques of microelectrophoresis and antidromic identification have been utilized in the female rat anesthetized with urethane to localize the tuberoinfundibular neurons and to determine their pharmacological sensitivity to dopamine (DA), norepinephrine (NE) and glutamate (GLUT). Extracellular recordings were made of 149 single units in the arcuate (ARC) nucleus; 79 of which were antidromically identified (43 were spontaneously active and 36 were silent) and the remaining 70 cells were determined to be spontaneously active but could not be antidromically identified (unidentified ARC neurons). The mean latency of the antidromically activated potentials was 9,5 msec, which gave a conduction velocity of 0.05 mm/msec. The spontaneous firing rate of antidromically identified ARC neurons varied from less than 1/sec to 8/sec while unidentified cells discharged at rates from less than 3/sec to 10/sec. These data indicate that ARC neurons exist which are capable of conducting impulses and send their axons into the external layer of the ME. Successful drug applications were made on 104 ARC neurons; of the 43 antidromically identified units, 37 were spontaneously active and 6 were silent. There were 61 spontaneously active, unidentified neurons. Two distinct pools of antidromically identified ARC neurons were found based on their sensitivity to NE and DA. Neurons displaying excitation to NE(N = 23) were either inhibited (N = 10) or non-responsive (N = 13) to DA applied electrophoretically; no NE-sensitive ARC neurons were excited by DA. The second pool of neurons (N = 14) was excited by DA. These neurons when tested with NE were either reproducibly inhibited (N = 3) or nonresponsive (N = 11); no DA-sensitive neurons were excited by NE. In the unidentified ARC neurons, iontophoretically applied NE and DA gave reproducible effects on individual neurons, so that some neurons were excited by both NE and DA, some were inhibited by both, and still others were excited by one and inhibited by the other chemical. Glutamate (GLUT) had a powerful excitant action on nearly all the neurons tested, increasing the electrical activity of the spontaneously active neurons as well as initiating activity in quiescent ARC neurons. These data are suggestive of a tuberoinfundibular neuronal system whose activity is modulated by either DA or NE. This evidence is certainly compatible with a functional role for both DA and NE in regulating pituitary function by altering the activity of presumed neurosecretory neurons which release hypophysiotropic hormones.
Motility changes in the antrum after proximal gastric vagotomy.
The normal pattern of resting and post-prandial motor activity in the gastric antrum has been established by observations in 6 dogs. There was a gradual increase in the amplitude of contraction during the first 2 hours after eating; this was maintained for 3 hours and then declined. For the first 45 minutes terminal antral contraction occurred, partially retaining and triturating the gastric contents. After 45 minutes the waves became sequential, symmetrical, increased in vigour and actively pumped food into the duodenum. Vagotomy modified the mechanism of the antrum in various ways. Truncal and selective vagotomy reduced the work capability to 20 per cent of its normal value when recorded 1 month after operation. In both groups the waves were disorganized. Proximal gastric vagotomy abolished the braking mechanism and removed the initial inhibitory stimuli to antral motility. Within 1 month of operation the antrum had regained 58 per cent of its normal work capability and the contractions were well organized.
Human therapeutic and agricultural uses of antibacterial drugs and resistance of the enteric flora of humans.
Fecal samples were collected from five groups of people differing in the manner of their exposure to antibacterial drugs. The groups included: (i) people working on farms who were continuously in contact with the predominantly resistant florae of farm animals receiving rations containing antibacterial drugs, (ii) people residing on the same farms with no direct exposure to the farm animals, (iii) people treated with antibacterial drugs, (iv) untreated people residing with treated individuals, and (v) untreated people with no exposure to farm animals or treated individuals. The samples were examined by quantitative plating for proportions of antibiotic-resistant, gram-negative enteric organisms. Individual isolates were also examined for their susceptibility to 11 different antibacterial drugs. The results indicate that enteric florae unexposed directly to the selective effects of antibacterial drugs may be affected by contact with predominantly resistant florae directly exposed to antibacterial drugs.
Birthright--alternative to abortion.
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Unusual ultrastructural features in alveolar rhabdomyosarcoma.
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Determination of the efficiency of utilization of lysine and methionine in fish meal for turkeys.
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Nerve conduction in childhood diabetes.
Sixty-nine diabetic children were studied with respect to the motor nerve conduction velocities, duration of illness and adequacy of control.As a group there was a trend for children with diabetes to have slower MNCV than non-diabetic children, and for the slowing to become progressive as the duration of their disease increased. Poorer quality of diabetes control was also associated with progressive slowing of conduction but the exact relationship is uncertain, since no patient who had diabetes for more than four years was well controlled.Theories of causation of peripheral neuropathy in diabetic patients are reviewed; metabolic changes, rather than other factors, are thought most likely in children.