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Biomedical subjects

M Kawanaka

Publications and source records attributed to M Kawanaka.

At least 37 records · Page 2Linked to original sources
PubMed1991

Identification of a putative eggshell precursor protein of the female Schistosoma japonicum.

In adult worms of Schistosoma japonicum, a prominent radiolabelled female-specific protein (34 kDa) was demonstrated on fluorography of SDS gels with the pulse incorporation of 14C-tyrosine in vitro, though it was difficult to detect major female-specific proteins by direct staining methods. This female-specific protein was demonstrated to localize exclusively in the vitelline cells by indirect immunofluorescence using the rabbit anti-34 kDa female protein antiserum. It was shown that 14C-tyrosine was selectively incorporated into the vitelline cells by the pulse labelled autoradiographs. Two days after the exposure of worms to radio-tyrosine, the shells of eggs in the uterus were demonstrated to have become radioactive, indicating that 14C-tyrosine-labelled protein was used as a material for the eggshell. In the fluorograph of proteins extracted from newly laid eggs in vitro, the prominent band was not found at the 34 kDa region, but a lot of radioactivity appeared at higher than 100 kDa. The results suggested that a 34 kDa female protein was a precursor of the eggshell and became a much larger protein molecule as a result of cross-linking during eggshell hardening.

Animals↗
PubMed1990

[Development of the dental CAD/CAM system].

Studies have been undertaken to apply CAD/CAM system to Dentistry and to make prosthetic appliances with this system automatically. Specimens are 4 times large plaster models. For the inside of the crown, the plaster model of prepared tooth is measured with laser displacement meter then the numerical data is obtained. After modification of this data for the concave cutting, the modeling machine works with this numerical data. For the outside of the crown, the typical colonal figure data (= CAD Data Base) is prepared. And this data is modified with computer to fit the prepared tooth margin and proximal or antagonical tooth (= CAD). This CAD Data Base was obtained with 3 dimensional point digitizer (3DPD). Because this measuring method with 3DPD is to be able to select points, the CAD Data Base could be consists of characteristic points. When this data base is really used, it is interpolated with s-spline. Spline interpolation is indispensable to the CAD/CAM system. Further understanding of this system, explanation is divided into three parts which are 3D measurement, CAD and CAM. (3D measurement) Two types of 3D measurement is dealed with this system. One is for the CAD data base and another is for the prepared tooth model. 3D measurement of the prepared tooth model is equivalent of the impression takings in the routine method. For the clear marginal line and for the uniform distribution of measuring points, the prepared tooth model is tilted and rotated on the working table when it is measured with laser. (CAD) The CAD Data Base can be extended, contracted, parallel translated and rotated with the Affine transformation. For putting the individual margin data on the CAD Data Base, the prepared tooth margin is re-digitized with 3DPD. Occlusal data is taken from F.G.P. core. (CAM) The application of the spline interpolation to the tool offset theory, which is effective at the groove especially, makes easy to calculate the tool path. When the prepared tooth model is manufactured, it is tilted and rotated on the table like the measurement with laser-scan.

Crowns↗
PubMed1989

Clinical application of SPECT in adrenal imaging with iodine-131 6 beta-iodomethyl-19-norcholesterol.

Forty-one patients with or without adrenocortical disorders were studied to evaluate the clinical usefulness of SPECT in adrenal imaging with I-131 Adosterol. In the SPECT images from this study, all glands with either normally functioning or hyperfunctioning adrenal cortices could be detected, while those glands with hypofunctioning adrenal cortices could not be detected. Particularly in transaxial and sagittal slices, the adrenal gland was identified posteriorly and was clearly distinguished from the gallbladder. In preliminary results using SPECT by a standard method, uptake in 68 detectable glands ranged from 1.7% to 4.9% in four glands with Cushing's syndrome, from 1.1% to 1.3% in seven glands with primary aldosteronism, and were distributed below 1.0% in the remaining glands with normally functioning adrenal cortices. These data show that it is possible to evaluate the adrenocortical functioning status simply by analyzing the SPECT images of the adrenal.

Adosterol↗
PubMed1989

Glucose metabolism of adult Schistosoma japonicum as revealed by nuclear magnetic resonance spectroscopy with D-[13C6]glucose.

Excretory end-products of adult Schistosoma japonicum, fed D-[13C6]glucose in vitro under aerobic and anaerobic conditions, were studied using 1H- and 13C-nuclear magnetic resonance (NMR) spectroscopy. The glucose in the medium is degraded to produce lactate and alanine aerobically and succinate and acetate as well as lactate and alanine anaerobically. Succinate and acetate have not been previously recorded as excretory products resulting from the metabolism of glucose for schistosomes. The presence of [13C3] and [2,3-13C2]lactate, and [1,2,2'-13C3] and [2,2'-13C2]succinate as end-products suggests that a partial reversed tricarboxylic acid (TCA) cycle is active in adult Schistosoma japonicum under anaerobic conditions. The physiological role of this pathway in adult schistosomes remains obscure.

Acetates↗
PubMed1989

[An approach to the dental CAD/CAM (Part 4). Fundamental CAD procedure for the data base].

Studies are currently underway to apply the CAD/CAM system to dentistry and to make prosthetic appliances automatically. This paper reports the basic CAD procedure by using the CAD data base in the previous (part 3) report and gives some modified teeth figures. Mathematically, with Affine transformation, extension, contraction, parallel translation, and rotation of the dental figures are done. In the transformation, the origin of the individual tooth is decided from the mean value of the data base points, and X, Y, Z coordinates are the bucco-lingal, mesio-distal, vertical directions, respectively. This basic transforming procedure reduces the time needed for the CAD procedure.

Computer Graphics↗
PubMed1983

Nutritional requirements of Schistosoma japonicum eggs.

Newly laid eggs of Schistosoma japonicum were cultured in a serum-free, chemically defined medium, RPMI 1640, which contained 20 amino acids, glutathione, 11 vitamins, and glucose in a balanced salt solution. The requirements for these components in the nutrition of the eggs was investigated by the deletion of single component from the medium. The following 14 amino acids were shown to be essential for the full development of the egg in the medium: L-arginine, L-cystine, glycine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-serine, L-threonine, L-tryptophan, L-tyrosine, and L-valine. Choline chloride was the essential vitamin. The omission of nicotinamide from the medium affected maturation adversely. Glucose was also required by the eggs. Minimal concentration of glucose for maturation of the eggs was 0.02 mM, but concentrations ranging from 0.16 to 20.00 mM gave better results while the concentration of the other elements of the medium were kept constant.

Amino Acids↗
PubMed1978

Effect of cytidine diphosphate choline on growth hormone and prolactin secretion in man.

The effect of intravenous infusion of cytidine diphosphate choline (CDP-choline) on the serum levels of growth hormone (GH) and prolactin (PRL) was studied in six normal adult male subjects. Serum GH levels increased and reached a maximum at 60--90 min after the initiation of infusion in all subjects examined. The mean peak value of GH in six subjects was 10.0 +/- 2.1 (mean +/- SE) ng/ml, which was signigicantly higher than the basal level (p less than 0.01). In four subjects, serum PRL levels decreased from 10--24 ng/ml to less than 7.2 ng/ml at 60--120 min, while in the other two no significant change was observed. These results indicate that CDP-choline affects GH and PRL secretion from the anterior pituitary.

Adolescent↗