Search PubMed⌕ Search

Biomedical subjects

M Kawamura

Publications and source records attributed to M Kawamura.

At least 739 records · Page 41Linked to original sources
PubMed1987

Alexia with agraphia of kanji (Japanese morphograms).

The case of the right-handed young Japanese woman with alexia with agraphia of kanji (the Japanese morphograms) due to a small circumscribed haematoma in the left posterior inferior temporal gyrus is described. Her chief complaint was the inability to read and write kanji. Detailed examination showed that her alexia with agraphia was much more predominant for kanji than kana (the Japanese syllabograms). These facts suggest that the processing of kanji and kana involves different intrahemispheric mechanisms.

Adult↗
PubMed1987

Diuretic response of a second infusion of atrial natriuretic polypeptide into anesthetized dogs.

We examined the diuretic effects of a second infusion of atrial natriuretic polypeptide (ANP). Intrarenal infusion of ANP (50 ng X kg-1 X min-1) for 30 min to anesthetized dogs had no significant effect on mean arterial blood pressure (MABP), renal blood flow (RBF) and glomerular filtration rate (GFR), whereas there was an increase in urine flow (V) and urinary excretion of sodium (UNaV) from 7.5 +/- 1.5 to 34.1 +/- 9.4 microliters/g X min, from 0.97 +/- 0.20 to 4.38 +/- 1.32 microEq/g X min, respectively. When the same dose of ANP was infused 1 h after the first administration, V and UNaV were increased from 12.4 +/- 1.8 to 62.5 +/- 18.8 microliters/g X min, from 1.72 +/- 0.57 to 7.18 +/- 2.68 microEq/g X min, respectively. A second infusion of ANP caused a greater absolute increase in UNaV than did the first infusion but proportionally the increase in UNaV with the second ANP infusion was much the same. The present study shows that acute ANP infusion has no tachyphylaxis on the natriuretic response. These data will aid in establishing a bioassay for natriuretic response in vivo, thereby leading to elucidation of the physiological action of ANP.

Animals↗
PubMed1987

Maternal blood distribution of zinc and copper during labor and after delivery.

In 15 normal mothers the stress effects of labor on zinc and copper distributions in erythrocytes and plasma were investigated. Lower levels of total zinc and of zinc derived from both carbonic anhydrase isoenzymes (CA-I and CA-II), especially from CA-I, in erythrocytes were obtained immediately after delivery. On the other hand, there was a significant increase in total plasma zinc concentration only on admission to hospital in labor, although albumin-bound zinc concentration remained elevated throughout the entire labor. These results suggest that there are unexplained disparities between the changes in zinc levels in maternal erythrocytes and plasma during labor.

Adult↗
PubMed1987

Renal function curve in patients with secondary forms of hypertension.

The causative mechanisms of hypertension were investigated by studying the renal function (pressure-natriuresis) curve in patients with primary aldosteronism (n = 6) and renovascular hypertension (n = 6). Before and after radical operation (removal of adenoma in primary aldosteronism and percutaneous transluminal angioplasty in renovascular hypertension), dietary NaCl intake was altered from 10 to 13 g/day in Week 1 to 1 to 3 g/day in Week 2. Mean arterial pressure (MAP) and urinary sodium excretion were measured on the last 3 days of each week. By restricting sodium intake before operation, MAP was reduced from 122 +/- 7 to 113 +/- 7 mm Hg (p less than 0.025) in primary aldosteronism but not in renovascular hypertension (130 +/- 6 to 128 +/- 5 mm Hg). The renal function curve was drawn by plotting urinary sodium excretion on the ordinate and MAP on the abscissa before and after operation. The slope of the curve was analyzed between the plotted points, and each curve was extrapolated to zero sodium excretion as an estimate of the degree of shift of the curve along the MAP axis. Before, as compared with after operation, the extrapolated x-intercept of the curve was shifted rightward in both primary aldosteronism (111 +/- 7 vs 87 +/- 4 mm Hg; p less than 0.025) and renovascular hypertension (128 +/- 5 vs 95 +/- 2 mm Hg; p less than 0.025) and the slope was depressed in primary aldosteronism (16 +/- 1 vs 40 +/- 17 [mEq/day]/mm Hg; p less than 0.025) but not in renovascular hypertension (130 +/- 75 vs 40 +/- 13 [mEq/day]/mm Hg).(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1987

Distribution of alpha 1- and alpha 2-adrenoceptors in brush border and basolateral membranes from rat kidney cortical tubules.

To examine the localization of alpha-adrenoceptors within the kidney tubule, binding experiments with 125I-HEAT and 3H-yohimbine were performed using cortical homogenates, basolateral membranes (BLM) and brush border membranes (BBM). Bmax values for 125I-HEAT or 3H-yohimbine binding were 2.3- or 2.0-fold that of the homogenate in BBM and 8.2- or 10.7-fold in BLM, respectively. Kd values for 125I-HEAT or 3H-yohimbine were not significantly different between BBM and BLM. These results indicate that both types of alpha-adrenoceptors seem to be localized largely in the BLM.

Animals↗
PubMed1987

Effects of atrial natriuretic peptide on renal function and renin release in the isolated perfused rat kidney.

The effects of synthetic atrial natriuretic peptide (ANP) on the renal hemodynamics, glomerular filtration rate (GFR), fluid and electrolyte excretion and renin release were studied in the isolated perfused rat kidney (IPK). When 10(-9) mol of ANP was administered in 75 ml of perfusate, the renal vascular resistance (RVR) was transiently decreased for 3 to 5 min, thereafter increased for 30 min and then tended to return to the control level. ANP increased the GFR (0.55 +/- 0.08 to 0.71 +/- 0.07 ml/min), urine flow (UV) (0.018 +/- 0.002 to 0.194 +/- 0.028 ml/min), absolute Na excretion (UNaV) (1.83 +/- 0.03 to 17.93 +/- 2.71 microEq/min) and absolute K excretion (UKV) (0.67 +/- 0.13 to 2.33 +/- 0.18 microEq/min). The addition of indomethacin or mefenamic acid to the perfusate before the administration of ANP exerted no influence on any of the effects of ANP. Renin release was inhibited by approximately 50% compared to the ANP-free control group. With the administration of ANP, UV and UNaV reached a peak 15-20 min after the GFR reached a peak and remained elevated after the GFR fell below the control level. These findings suggest that 10(-9) mol of ANP causes natriuresis and renin suppression in the IPK, and that the natriuresis is prostaglandin-independent and cannot be explained only by an increase in GFR.

Animals↗
PubMed1987

Anomeric preference in uptake of D-glucose and of D-galactose by rat lenses.

To determine the anomeric preference of uptake of D-glucose and of D-galactose by rat lenses, we crystallized alpha-, beta-D-[U-14C]glucose (720 microCi/mmol) and alpha-, beta-D-[U-14C]galactose (180 microCi/mmol) by our method and incubated them separately with rat lenses for 1 min, because of the short half-life of mutarotation of alpha-D-glucose (9.6 min) and of alpha-D-galactose (4.6 min) in HEPES medium at 30 degrees C. During aerobic incubation of rat lenses in HEPES medium containing radioactive alpha or beta anomer of D-glucose, there was no significant difference in the rate of uptake between alpha and beta anomers of D-glucose by rat lenses. However, 1.59 times greater incorporation of alpha-D-galactose was observed over that of beta-D-galactose under the same conditions.

Animals↗
PubMed1987

A sensitive method for precise measurement of endogenous angiotensins I, II&III in human plasma.

We measured endogenous angiotensins (ANGs) I, II&III using a system of extraction by Sep-Pak column followed by high performance liquid chromatography (HPLC) combined with radioimmunoassay (RIA). An excellent separation of ANGs was obtained by HPLC. The recovery of ANGs I, II&III was 80-84%, when these authentic peptides were added to 6 ml of plasma. The coefficient of variation of the ANGs was 0.04-0.09 for intra-assay and 0.08-0.13 for inter-assay, thereby indicating a good reproducibility. Plasma ANGs I, II&III measured by this method in 5 normal volunteers were 51,4.5 and 1.2 pg/ml. In the presence of captopril, ANGs II&III decreased by 84% and 77%, respectively, while ANG I increased 5.1 times. This method is therefore useful to assess the precise levels of plasma ANGs.

Angiotensin I↗
PubMed1987

Chondroosteogenetic response to crude bone matrix proteins bound to hydroxyapatite.

For the development of a new implantable biomaterial that possesses osteogenetic ability, bone matrix morphogenetic proteins were bound to hydroxyapatite (BMP-HAP). A crude protein extract including the bone morphogenetic protein (BMP) fraction was precipitated in the pores of hydroxyapatite (HAP) inside of a dialysis sac. In tissue culture, BMP-HAP or HAP was used as substratum for rat mesenchymal muscle cell explants. The BMP-HAP and HAP controls were also implanted in the muscle pouches of mice. New cartilage and/or new bone formation was observed on the exterior surface of BMP-HAP but not of HAP controls. Implantation of this HAP into bone marrow cavities of rabbit femoral and tibial condyle produced new bone formation. The deposits extended further inside BMP-HAP than HAP pores. The pore diameters of 90-200 microns produced earlier ingrowth than into larger pore sizes. BMP-HAP should be tested for clinical application as osteogenetic biomaterial for augmentation of bone regeneration.

Animals↗
PubMed1987

[Evaluation of rIFN-gamma in the treatment of lymphoma and melanoma of the skin by systemic and intralesional administration].

This study evaluated the clinical effect of rIFN-gamma for the treatment of lymphoma and melanoma of the skin by systemic intravenous and intralesional administration. By intravenous drip infusion, one of two cases of mycosis fungoides (stage IV) showed CR of skin lesions by administration of a total of 160 X 10(6) units, 4 or 8 X 10(6) units, every other day for 3 months. The remaining case was, however, in a state of PD. Intralesional administration of rIFN-gamma to six cases of cutaneous lymphoma, including three cases of mycosis fungoides, two cases of cutaneous T cell lymphoma, and one case of adult T cell lymphoma, resulted in three cases of CR, two cases of MR and one case of PD. In contrast, four cases of malignant melanoma, two of which received systemic administration and two intralesional administration, did not show any obvious clinical response, one showing PR and the other three PD. Histopathological examination of skin lesions before and after administration of rIFN-gamma indicated that the cutaneous lymphoma lesions were initially infiltrated by lymphocytes and macrophages, and that later on, they were free from tumor cells. Our study indicates that rIFN-gamma appears to provide a new modality for the treatment of cutaneous lymphoma by systemic and intralesional administration.

Administration, Cutaneous↗