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Biomedical subjects

M Katoh

Publications and source records attributed to M Katoh.

At least 397 records · Page 22Linked to original sources
PubMed1981

Anticancer drug sensitivity in vitro in the bladder cancer cell line, KK-47 and prophylactic use of carbazilquinone and urokinase in bladder cancer.

Using colony formation technique and KK-47 cell line established from a human bladder transitional cell carcinoma, the effect of 6 anticancer drugs, thio-TEPA, Bleomycin, mitomycin C, carbazilquinone, Adriamycin and cis-Platinum, were compared. On the results of tests performed to establish the drug concentration required to achive a 50% inhibition of cell survival, carbazilquinone was chosen for the prevention of recurrences of bladder cancer. The two groups studied consisted of 56 patients (previously untreated groups) who were rendered free of tumours by surgical intervention and of 19 patients (thio-TEPA failures group) who had experienced a persistent recurrence of tumours after prophylactic thio-TEPA instillations and were presumed free of the recurrence of tumours after the next surgical intervention. The 2 groups were subjected to prophylactic combined intravesical instillation therapy with carbazilquinone and urokinase. In the previously untreated group, 6 of the 56 patients (10.7%) had a recurrence of tumours, and the recurrence rate after 21 months was 16.7%, using the actuarial method. In the thio-TEPA failures group, 12 of the 19 patients (63.2%) had a recurrence of tumours, a rate at 21 months of 76.1%. A considerable drop in the recurrence rate was obtained by the combined instillation therapy in the previously untreated group. The results in the thio-TEPA failures group suggested the presence of a cross-resistance between both alkylating agents, and of a persistent susceptibility to multifocal lesions. No bone marrow depression was observed but an episode of anaphylactic shock attributable to the use of carbazilquinone occurred in 1 out of a total 75 patients.

Adult↗
PubMed1981

Characterization of surface markers and cytoplasmic organelle in benign and malignant lymphoid lesions of skin; immunohistochemical and electron microscopic evaluation.

In order to characterize the benign and malignant proliferation of lymphoid cells in skin, we compared surface markers and cytoplasmic organelles of cells in cutaneous lymphoid hyperplasia (CLH), lymphomatoid papulosis (LP), mycosis fungoides (MF), Sézary's syndrome (SS) and primary cutaneous malignant lymphoma (ML). The immunohistochemical study showed cells with both T - and B-cell markers in CLH,LP and early MF, whereas cells with only the T-cell marker were seen in late MF, SS and ML. T-cells in all cutaneous lesions possessed the surface marker common to T-cells of peripheral lymph nodes, and not that of central thymus cells. Cutaneous T-cells contained clustered or scattered dense core granules. Although no specific organelles indicative of benign or malignant lymphoid proliferation were found, there were several ultrastructural features that could help identifying each form of cutaneous lymphoid lesions. These included clustered or scattered dense-core granules, the variable degree of nuclear convolutions as well as dendritic arborization, and the presence or absence of 10 nm filaments.

Adolescent↗
PubMed1981

Formation of chromosome-type aberrations at the first cleavage after MMS treatment in late spermatids of mice.

Male mice were treated with graded doses of methyl methanesulfonate (MMS) and mated with untreated female mice during the six to ten days after treatment. The nature of the primary lesions induced in late spermatids was investigated by analysing the types and rates of chromosome aberrations in the first cleavage metaphases. The chromosome aberrations recovered at the first cleavage were predominantly of the chromosome-type. Their frequency increased exponentially with increasing dose of MMS. The results suggested that the nonenzymatic conversion of DNA containing alkylated bases to new damage, possibly strand breaks, during the maturation and storage of sperm was primarily responsible for the enhancement of the frequency of chromosome-type aberrations.

Animals↗
PubMed1980

Effect of diisopropyl 1,3-dithiol-2-ylidenemalonate on microsomal electron transport system in rat liver.

Effect of diisopropyl 1,3-dithiol-2-ylidenemalonate (NKK-105) on the components of rat liver microsomal electron transport system was investigated by comparison with those of phenobarbital, 3-methylcholanthrene and polychlorinated biphenyl. When NKK-105 was administered to rats at a dose of 250 mg/kg/day for 7 days, cytochrome b5 content and NADPH-cytochrome c reductase activity were significantly increased but cytochrome P-450 content to the lesser extent. Three inducers of the drug metabolizing enzymes remarkably increased cytochrome P-450 content but increased cytochrome b5 content to a lesser extent.

Animals↗
PubMed1978

Mutagenicity of benzotrichloride and related compounds.

Benzotrichloride (BTC), benzal chloride (BDC), benzyl chloride (BC) and benzoyl chloride (BOC) were surveyed for their mutagenicity in microbial systems such as rec-assay using Bacillus subtilis and reversion assays using E. coli WP2 and Ames Salmonella TA strains with or without metabolic activation in vitro. BTC and BDC required metabolic activation for their mutagenic activities in several strains of E. coli and Salmonella. The mutagenic metabolites of these compounds may not have been produced by hydrolysis. BC was weakly mutagenic without metabolic activation. Only BOC exhibited no mutagenic activity in the detection procedures used. The mutagenic metabolite of BTC might be very unstable under our experimental conditions. The strain E. coli WP2 try hcr was more sensitive than E. coli B/r WP2 try (hcr+) with regard to the mutagenicity of BTC.

Bacillus subtilis↗
PubMed1978

[Effect of synthetic motilin on gastric motility (author's transl)].

The effects of the synthetic motilin on the gastric motility were investigated in the dogs with innervated antral pouches in fasting state. The results were as follows: 1) The synthetic motilin stimulated markedly the gastric motor activity in both the contraction pressure and the frequency of the peristalsis. 2) Tetragastrin produced more regular and higher frequent contractions compared with synthetic motilin, but acted to weaker the contraction pressure. 3) In simultaneous administration of the synthetic motilin and the tetragastrin, the synthetic motilin acted more strongly in the contraction pressure, on the other hand tetragastrin in the frequency of the peristalsis. 4) The peristaltic contractions induced by synthetic motilin were completely abolished by the administration of the atropine sulfate. 5) Under medical vagotomy, synthetic motilin was not able to produce any peristatlic contractions, but tetragastrin stimulated strongly the gastric motility.

Animals↗